Optimization of the enzymatic hydrolysis of starch in sugarcane juice (Saccharum spp hybrid)

ABSTRACT Starch, a natural component of sugarcane juice, causes losses and operational problems in the sugar industry, due to the increase in juice viscosity, which inhibits crystallization and increases sucrose loss. This research aimed to optimize the enzymatic hydrolysis process of starch by the action of Bacillus licheniformis alpha-amylase. Enzyme concentration and reaction time were optimized for maximizing maltose concentration, determined as reducing sugar by the Dinitrosalicylic Acid method; starch hydrolysis percentage and productivity, which were analyzed independently and together by a Rotational Compound Central Design and Response Surface Methodology. The alpha-amylase enzyme was characterized, obtaining an optimum temperature of 90 °C and optimum pH of 7, determining a linearity range for the 1:100 dilution of 20 minutes, an enzymatic activity of 28.35 IU/mg, and the kinetic constants Km of 5.82 g/L and Vmax of 0.30 g/L*min. Finally, the experimental design obtained the optimal environmental conditions of enzyme concentration of 817 ppm and reaction time of 17 minutes at a temperature of 90 °C. From the validation of the optimal conditions for starch hydrolysis in sugarcane juice, the following were obtained: maltose concentration 0.380 g/L, percentage of hydrolysis 73.09 %, and productivity 1.341 g/L*h, which corresponded to variations greater than the calculated values of 16%, 11.3%, and 18%, respectively.

Bibliographic Details

Main Authors:	Yanamango Chavez,V., Morales Zúñiga,M., Castillo Calderón,A., Diestra-Balta,J.
Format:	Digital revista
Language:	English
Published:	Universidad Autónoma de Nayarit 2023
Online Access:	http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-33802023000100434
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