Usefulness of Fourier transform infrared (FTIR) spectroscopy to detect Trichinella spiralis (Owen, 1835) muscle larvae in ham and sausages made from the meat of an experimentally infected pig
Abstract The aim of this work was to determine the usefulness of Fourier transform infrared (FTIR) spectroscopy to detect muscle larvae of Trichinella spiralis (Owen, 1835) in ham and sausages made from the meat of an experimentally infected pig. It was searched for the muscle larvae (ML) by conventional methods (artificial digestion and trichinoscopy stained with Mayer´s hemalum stain) and by FTIR spectroscopy. In addition, the infective capacity of the larvae found in swine products was analyzed. The parasite load was 8.5 ± 3 mL/g in ham and 4.5 ± 1.4 mL/g in sausage (P<0.0001). The spectra of the pig products prepared with the meat of an uninfected pig were different in the range of 1,700 to 900 cm-1 with respect to the spectra of the products from infected pigs. In this region, glycogen is the most abundant chemical group (1,200 and 900 cm-1). The distance between classes between non-infested and infested products was 10.2 for ham and 5.52 for sausages (three is the minimum value to indicate class separation). The infective capacity of the larvae recovered from pig products decreased up to five times compared to that of the larvae obtained from experimentally infected mice. These results show that the FTIR spectroscopy is useful to determine the presence of T. spiralis larvae in the foods studied here. Further studies are needed to determine the influence of meat flavors on the detection of Trichinella by FTIR spectroscopy.
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Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
2021
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oai:scielo:S2007-112420210004011332022-06-06Usefulness of Fourier transform infrared (FTIR) spectroscopy to detect Trichinella spiralis (Owen, 1835) muscle larvae in ham and sausages made from the meat of an experimentally infected pigRosa Arana,Jorge Luis de laPonce Noguez,Jesús BenjamínGallardo Velázquez,TzayhriReyes Rodríguez,Nydia EdithZepeda Velázquez,Andrea PalomaLópez Lugo,Ana BereniceSánchez Paredes,Alejandro PabloMartínez Labat,PabloGómez de Anda,Fabián Ricardo Trichinella Meat-inspection Sausage Diagnosis Infrared-spectroscopy Abstract The aim of this work was to determine the usefulness of Fourier transform infrared (FTIR) spectroscopy to detect muscle larvae of Trichinella spiralis (Owen, 1835) in ham and sausages made from the meat of an experimentally infected pig. It was searched for the muscle larvae (ML) by conventional methods (artificial digestion and trichinoscopy stained with Mayer´s hemalum stain) and by FTIR spectroscopy. In addition, the infective capacity of the larvae found in swine products was analyzed. The parasite load was 8.5 ± 3 mL/g in ham and 4.5 ± 1.4 mL/g in sausage (P<0.0001). The spectra of the pig products prepared with the meat of an uninfected pig were different in the range of 1,700 to 900 cm-1 with respect to the spectra of the products from infected pigs. In this region, glycogen is the most abundant chemical group (1,200 and 900 cm-1). The distance between classes between non-infested and infested products was 10.2 for ham and 5.52 for sausages (three is the minimum value to indicate class separation). The infective capacity of the larvae recovered from pig products decreased up to five times compared to that of the larvae obtained from experimentally infected mice. These results show that the FTIR spectroscopy is useful to determine the presence of T. spiralis larvae in the foods studied here. Further studies are needed to determine the influence of meat flavors on the detection of Trichinella by FTIR spectroscopy.info:eu-repo/semantics/openAccessInstituto Nacional de Investigaciones Forestales, Agrícolas y PecuariasRevista mexicana de ciencias pecuarias v.12 n.4 20212021-12-01info:eu-repo/semantics/articletext/htmlhttp://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-11242021000401133en10.22319/rmcp.v12i4.5897 |
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Rosa Arana,Jorge Luis de la Ponce Noguez,Jesús Benjamín Gallardo Velázquez,Tzayhri Reyes Rodríguez,Nydia Edith Zepeda Velázquez,Andrea Paloma López Lugo,Ana Berenice Sánchez Paredes,Alejandro Pablo Martínez Labat,Pablo Gómez de Anda,Fabián Ricardo |
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Rosa Arana,Jorge Luis de la Ponce Noguez,Jesús Benjamín Gallardo Velázquez,Tzayhri Reyes Rodríguez,Nydia Edith Zepeda Velázquez,Andrea Paloma López Lugo,Ana Berenice Sánchez Paredes,Alejandro Pablo Martínez Labat,Pablo Gómez de Anda,Fabián Ricardo Usefulness of Fourier transform infrared (FTIR) spectroscopy to detect Trichinella spiralis (Owen, 1835) muscle larvae in ham and sausages made from the meat of an experimentally infected pig |
author_facet |
Rosa Arana,Jorge Luis de la Ponce Noguez,Jesús Benjamín Gallardo Velázquez,Tzayhri Reyes Rodríguez,Nydia Edith Zepeda Velázquez,Andrea Paloma López Lugo,Ana Berenice Sánchez Paredes,Alejandro Pablo Martínez Labat,Pablo Gómez de Anda,Fabián Ricardo |
author_sort |
Rosa Arana,Jorge Luis de la |
title |
Usefulness of Fourier transform infrared (FTIR) spectroscopy to detect Trichinella spiralis (Owen, 1835) muscle larvae in ham and sausages made from the meat of an experimentally infected pig |
title_short |
Usefulness of Fourier transform infrared (FTIR) spectroscopy to detect Trichinella spiralis (Owen, 1835) muscle larvae in ham and sausages made from the meat of an experimentally infected pig |
title_full |
Usefulness of Fourier transform infrared (FTIR) spectroscopy to detect Trichinella spiralis (Owen, 1835) muscle larvae in ham and sausages made from the meat of an experimentally infected pig |
title_fullStr |
Usefulness of Fourier transform infrared (FTIR) spectroscopy to detect Trichinella spiralis (Owen, 1835) muscle larvae in ham and sausages made from the meat of an experimentally infected pig |
title_full_unstemmed |
Usefulness of Fourier transform infrared (FTIR) spectroscopy to detect Trichinella spiralis (Owen, 1835) muscle larvae in ham and sausages made from the meat of an experimentally infected pig |
title_sort |
usefulness of fourier transform infrared (ftir) spectroscopy to detect trichinella spiralis (owen, 1835) muscle larvae in ham and sausages made from the meat of an experimentally infected pig |
description |
Abstract The aim of this work was to determine the usefulness of Fourier transform infrared (FTIR) spectroscopy to detect muscle larvae of Trichinella spiralis (Owen, 1835) in ham and sausages made from the meat of an experimentally infected pig. It was searched for the muscle larvae (ML) by conventional methods (artificial digestion and trichinoscopy stained with Mayer´s hemalum stain) and by FTIR spectroscopy. In addition, the infective capacity of the larvae found in swine products was analyzed. The parasite load was 8.5 ± 3 mL/g in ham and 4.5 ± 1.4 mL/g in sausage (P<0.0001). The spectra of the pig products prepared with the meat of an uninfected pig were different in the range of 1,700 to 900 cm-1 with respect to the spectra of the products from infected pigs. In this region, glycogen is the most abundant chemical group (1,200 and 900 cm-1). The distance between classes between non-infested and infested products was 10.2 for ham and 5.52 for sausages (three is the minimum value to indicate class separation). The infective capacity of the larvae recovered from pig products decreased up to five times compared to that of the larvae obtained from experimentally infected mice. These results show that the FTIR spectroscopy is useful to determine the presence of T. spiralis larvae in the foods studied here. Further studies are needed to determine the influence of meat flavors on the detection of Trichinella by FTIR spectroscopy. |
publisher |
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias |
publishDate |
2021 |
url |
http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-11242021000401133 |
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