In vitro production of porcine embryos with use of chemically semi-defined culture media system

In vitro production of porcine embryos with use of chemically semi-defined culture media system

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Abstract The objective of this study was to determine the effect of a semi-defined culture media system developed in laboratory, named Pigs Media System (PMS) on the in vitro production of porcine embryos. In a first assay, the cummulus-oocytes complexes (COCs) were matured, fertilized and cultured for embryo development in PMS supplemented with bovine serum albumin (BSA), and in North Carolina State University-23 (NCSU-23) supplemented with follicular fluid, until blastocysts evaluation. In the assay 2, maturation and culture were performed in PMS using BSA or polyvinyl alcohol (PVA) in a 2 × 2 factorial arrangement (PMS-BSA/BSA, PMS-BSA/PVA, PMS-PVA/PVA, PMS-PVA/BSA). The PMS had a positive effect on the total cell number (58.04) and the decrease of the total lipids (49.4 %) regarding the NCSU-23 medium (37.98 and 59.2 % respectively; P<0.05). The percentage of monospermic fertilization was significantly lower (42.3 %; P<0.05) when oocytes were matured with PMS-BSA than in PMS-PVA (52.6 %). The supplementation of BSA in the PMS for embryo culture, increased the blastocyst development, the cell number of blastocysts and decreased the content of total lipids (36.8 %, 46.9 and 49.6 % respectively; P<0.05), in comparison with the supplementation of PVA in the PMS for embryo culture. These results suggest that the semi-defined culture media system developed by the National Genetic Resources Center (CNRG), have proved favorable effects on the total cell number and the decrease of total lipids of porcine blastocysts in vitro produced.

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Main Authors: Urbán Duarte,David, Álvarez Gallardo,Horacio, Pérez Reynozo,Sandra, Torre Sánchez,José Fernando De la
Format: Digital revista
Language:English
Published: Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias 2020
Online Access:http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-11242020000100102
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spelling oai:scielo:S2007-112420200001001022020-06-10In vitro production of porcine embryos with use of chemically semi-defined culture media systemUrbán Duarte,DavidÁlvarez Gallardo,HoracioPérez Reynozo,SandraTorre Sánchez,José Fernando De la Culture media Pigs Embryo In vitro production Abstract The objective of this study was to determine the effect of a semi-defined culture media system developed in laboratory, named Pigs Media System (PMS) on the in vitro production of porcine embryos. In a first assay, the cummulus-oocytes complexes (COCs) were matured, fertilized and cultured for embryo development in PMS supplemented with bovine serum albumin (BSA), and in North Carolina State University-23 (NCSU-23) supplemented with follicular fluid, until blastocysts evaluation. In the assay 2, maturation and culture were performed in PMS using BSA or polyvinyl alcohol (PVA) in a 2 × 2 factorial arrangement (PMS-BSA/BSA, PMS-BSA/PVA, PMS-PVA/PVA, PMS-PVA/BSA). The PMS had a positive effect on the total cell number (58.04) and the decrease of the total lipids (49.4 %) regarding the NCSU-23 medium (37.98 and 59.2 % respectively; P<0.05). The percentage of monospermic fertilization was significantly lower (42.3 %; P<0.05) when oocytes were matured with PMS-BSA than in PMS-PVA (52.6 %). The supplementation of BSA in the PMS for embryo culture, increased the blastocyst development, the cell number of blastocysts and decreased the content of total lipids (36.8 %, 46.9 and 49.6 % respectively; P<0.05), in comparison with the supplementation of PVA in the PMS for embryo culture. These results suggest that the semi-defined culture media system developed by the National Genetic Resources Center (CNRG), have proved favorable effects on the total cell number and the decrease of total lipids of porcine blastocysts in vitro produced.info:eu-repo/semantics/openAccessInstituto Nacional de Investigaciones Forestales, Agrícolas y PecuariasRevista mexicana de ciencias pecuarias v.11 n.1 20202020-03-01info:eu-repo/semantics/articletext/htmlhttp://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-11242020000100102en10.22319/rmcp.v11i1.4757
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libraryname SciELO
language English
format Digital
author Urbán Duarte,David
Álvarez Gallardo,Horacio
Pérez Reynozo,Sandra
Torre Sánchez,José Fernando De la
spellingShingle Urbán Duarte,David
Álvarez Gallardo,Horacio
Pérez Reynozo,Sandra
Torre Sánchez,José Fernando De la
In vitro production of porcine embryos with use of chemically semi-defined culture media system
author_facet Urbán Duarte,David
Álvarez Gallardo,Horacio
Pérez Reynozo,Sandra
Torre Sánchez,José Fernando De la
author_sort Urbán Duarte,David
title In vitro production of porcine embryos with use of chemically semi-defined culture media system
title_short In vitro production of porcine embryos with use of chemically semi-defined culture media system
title_full In vitro production of porcine embryos with use of chemically semi-defined culture media system
title_fullStr In vitro production of porcine embryos with use of chemically semi-defined culture media system
title_full_unstemmed In vitro production of porcine embryos with use of chemically semi-defined culture media system
title_sort in vitro production of porcine embryos with use of chemically semi-defined culture media system
description Abstract The objective of this study was to determine the effect of a semi-defined culture media system developed in laboratory, named Pigs Media System (PMS) on the in vitro production of porcine embryos. In a first assay, the cummulus-oocytes complexes (COCs) were matured, fertilized and cultured for embryo development in PMS supplemented with bovine serum albumin (BSA), and in North Carolina State University-23 (NCSU-23) supplemented with follicular fluid, until blastocysts evaluation. In the assay 2, maturation and culture were performed in PMS using BSA or polyvinyl alcohol (PVA) in a 2 × 2 factorial arrangement (PMS-BSA/BSA, PMS-BSA/PVA, PMS-PVA/PVA, PMS-PVA/BSA). The PMS had a positive effect on the total cell number (58.04) and the decrease of the total lipids (49.4 %) regarding the NCSU-23 medium (37.98 and 59.2 % respectively; P<0.05). The percentage of monospermic fertilization was significantly lower (42.3 %; P<0.05) when oocytes were matured with PMS-BSA than in PMS-PVA (52.6 %). The supplementation of BSA in the PMS for embryo culture, increased the blastocyst development, the cell number of blastocysts and decreased the content of total lipids (36.8 %, 46.9 and 49.6 % respectively; P<0.05), in comparison with the supplementation of PVA in the PMS for embryo culture. These results suggest that the semi-defined culture media system developed by the National Genetic Resources Center (CNRG), have proved favorable effects on the total cell number and the decrease of total lipids of porcine blastocysts in vitro produced.
publisher Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
publishDate 2020
url http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-11242020000100102
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