Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico

Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico

Abstract: Introduction: Sugarcane is an essential agricultural product for bioethanol production in Mexico. The discovery of both the bacterial community associated with this crop and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. Culture enrichment allows for the identification of the biodiversity of biological samples. The objective of this research was to identify the bacterial biodiversity related with two complex carbohydrate sources (starch and cellulose) in soils sown with sugarcane in the Papaloapan Basin in Oaxaca, Mexico via a metagenomic approach. Method: Soil content was analyzed chemically. Liquid LB, LB-starch and LB-1% carboximetilcellulose media were inoculated with 2 g soil and cultured at 180 rpm, 37°C for 48 h. The biomass was collected and the 16S rDNA gene was amplified and a library was constructed which was analyzed by sequencing. Results: N, K and Zn content of organic matter showed higher values than average, as opposed to P and Na, which were lower than average. In the library, 35 OTUs related to Clostridium, Bacillus, Enterococcus, Lysinibacillus and Citrobacter genera were found which could contain genes for breaking cellulose and starch. Discussion or Conclusion: This is the first approach to identify the diversity related to starch and cellulose hydrolysis in the Papaloapan region, where the principal genera detected were Clostridium, Bacillus, Enterococcus, Citrobacter and Lysinibacillus in a soil moderately rich in organic matter.

Saved in:
Bibliographic Details
Main Authors: Cortés López,N. G., Sachman Ruiz,B., Montor Antonio,J. J., Miranda Sánchez,F., Alcántara Hernández,R. J., Moral,S. del
Format: Digital revista
Language:English
Published: Universidad de La Salle Bajío A. C., Coordinación de Investigación 2018
Online Access:http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-07052018000100222
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S2007-07052018000100222
record_format ojs
spelling oai:scielo:S2007-070520180001002222019-03-28Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of MexicoCortés López,N. G.Sachman Ruiz,B.Montor Antonio,J. J.Miranda Sánchez,F.Alcántara Hernández,R. J.Moral,S. del bacterial biodiversity sugarcane enriched culture OTUs Abstract: Introduction: Sugarcane is an essential agricultural product for bioethanol production in Mexico. The discovery of both the bacterial community associated with this crop and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. Culture enrichment allows for the identification of the biodiversity of biological samples. The objective of this research was to identify the bacterial biodiversity related with two complex carbohydrate sources (starch and cellulose) in soils sown with sugarcane in the Papaloapan Basin in Oaxaca, Mexico via a metagenomic approach. Method: Soil content was analyzed chemically. Liquid LB, LB-starch and LB-1% carboximetilcellulose media were inoculated with 2 g soil and cultured at 180 rpm, 37°C for 48 h. The biomass was collected and the 16S rDNA gene was amplified and a library was constructed which was analyzed by sequencing. Results: N, K and Zn content of organic matter showed higher values than average, as opposed to P and Na, which were lower than average. In the library, 35 OTUs related to Clostridium, Bacillus, Enterococcus, Lysinibacillus and Citrobacter genera were found which could contain genes for breaking cellulose and starch. Discussion or Conclusion: This is the first approach to identify the diversity related to starch and cellulose hydrolysis in the Papaloapan region, where the principal genera detected were Clostridium, Bacillus, Enterococcus, Citrobacter and Lysinibacillus in a soil moderately rich in organic matter.info:eu-repo/semantics/openAccessUniversidad de La Salle Bajío A. C., Coordinación de InvestigaciónNova scientia v.10 n.20 20182018-01-01info:eu-repo/semantics/articletext/htmlhttp://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-07052018000100222en10.21640/ns.v10i20.1314
institution SCIELO
collection OJS
country México
countrycode MX
component Revista
access En linea
databasecode rev-scielo-mx
tag revista
region America del Norte
libraryname SciELO
language English
format Digital
author Cortés López,N. G.
Sachman Ruiz,B.
Montor Antonio,J. J.
Miranda Sánchez,F.
Alcántara Hernández,R. J.
Moral,S. del
spellingShingle Cortés López,N. G.
Sachman Ruiz,B.
Montor Antonio,J. J.
Miranda Sánchez,F.
Alcántara Hernández,R. J.
Moral,S. del
Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico
author_facet Cortés López,N. G.
Sachman Ruiz,B.
Montor Antonio,J. J.
Miranda Sánchez,F.
Alcántara Hernández,R. J.
Moral,S. del
author_sort Cortés López,N. G.
title Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico
title_short Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico
title_full Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico
title_fullStr Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico
title_full_unstemmed Starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the Papaloapan Basin, a megadiverse region of Mexico
title_sort starch- and cellulose-related microbial diversity of soil sown with sugarcane crops in the papaloapan basin, a megadiverse region of mexico
description Abstract: Introduction: Sugarcane is an essential agricultural product for bioethanol production in Mexico. The discovery of both the bacterial community associated with this crop and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. Culture enrichment allows for the identification of the biodiversity of biological samples. The objective of this research was to identify the bacterial biodiversity related with two complex carbohydrate sources (starch and cellulose) in soils sown with sugarcane in the Papaloapan Basin in Oaxaca, Mexico via a metagenomic approach. Method: Soil content was analyzed chemically. Liquid LB, LB-starch and LB-1% carboximetilcellulose media were inoculated with 2 g soil and cultured at 180 rpm, 37°C for 48 h. The biomass was collected and the 16S rDNA gene was amplified and a library was constructed which was analyzed by sequencing. Results: N, K and Zn content of organic matter showed higher values than average, as opposed to P and Na, which were lower than average. In the library, 35 OTUs related to Clostridium, Bacillus, Enterococcus, Lysinibacillus and Citrobacter genera were found which could contain genes for breaking cellulose and starch. Discussion or Conclusion: This is the first approach to identify the diversity related to starch and cellulose hydrolysis in the Papaloapan region, where the principal genera detected were Clostridium, Bacillus, Enterococcus, Citrobacter and Lysinibacillus in a soil moderately rich in organic matter.
publisher Universidad de La Salle Bajío A. C., Coordinación de Investigación
publishDate 2018
url http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-07052018000100222
work_keys_str_mv AT corteslopezng starchandcelluloserelatedmicrobialdiversityofsoilsownwithsugarcanecropsinthepapaloapanbasinamegadiverseregionofmexico
AT sachmanruizb starchandcelluloserelatedmicrobialdiversityofsoilsownwithsugarcanecropsinthepapaloapanbasinamegadiverseregionofmexico
AT montorantoniojj starchandcelluloserelatedmicrobialdiversityofsoilsownwithsugarcanecropsinthepapaloapanbasinamegadiverseregionofmexico
AT mirandasanchezf starchandcelluloserelatedmicrobialdiversityofsoilsownwithsugarcanecropsinthepapaloapanbasinamegadiverseregionofmexico
AT alcantarahernandezrj starchandcelluloserelatedmicrobialdiversityofsoilsownwithsugarcanecropsinthepapaloapanbasinamegadiverseregionofmexico
AT moralsdel starchandcelluloserelatedmicrobialdiversityofsoilsownwithsugarcanecropsinthepapaloapanbasinamegadiverseregionofmexico
_version_ 1756229614348271616

Similar Items