In vitro assessment for cytotoxicity screening of new antimalarial candidates
Abstract In antimalarial research there are no standard procedures to determine the toxicity of a drug candidate. Among the alternatives available, in vitro cytotoxicity assays are the most widely used to predict toxic effects of future therapeutic products. They have the advantage over the in vivo assays, in that they offer the possibility to restrain the number of experimental variables. The objective of the present study was to compare in vitro cytotoxic methods by testing various compounds currently used to treat malaria against different cell lines. Neutral red (NR) uptake and methylthiazoletetrazolium (MTT) colorimetric in vitro assays were used to determine preliminary toxicity of commercially available antimalarial drugs against tumor and non-tumor cells lines. Toxicity through brine shrimp lethality bioassay and hemolytic activity were also evaluated. Significant differences were observed in the tests measured by NR uptake. The tumor cell lines TOV-21G and HepG2 and non-tumor WI-26VA4 cells showed relatively uniform toxicity results, with TOV-21G being the most sensitive cell tested, presenting the lowest concentration to cause death to 50% of viable cells (CC50) values. The results of this study support the use of TOV-21G, HepG2 and WI-26VA4 cells lines as the choice for cytotoxicity tests to evaluate potential bioactive compounds.
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Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
2022
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oai:scielo:S1984-825020220001005402022-04-18In vitro assessment for cytotoxicity screening of new antimalarial candidatesEspíndola,Mariana RodriguesVarotti,Fernando de PillaAguiar,Anna Caroline CamposAndrade,Silmara NunesRocha,Eliana Maria Mauricio da Antimalarial Cytotoxicity assay MTT Neutral red Abstract In antimalarial research there are no standard procedures to determine the toxicity of a drug candidate. Among the alternatives available, in vitro cytotoxicity assays are the most widely used to predict toxic effects of future therapeutic products. They have the advantage over the in vivo assays, in that they offer the possibility to restrain the number of experimental variables. The objective of the present study was to compare in vitro cytotoxic methods by testing various compounds currently used to treat malaria against different cell lines. Neutral red (NR) uptake and methylthiazoletetrazolium (MTT) colorimetric in vitro assays were used to determine preliminary toxicity of commercially available antimalarial drugs against tumor and non-tumor cells lines. Toxicity through brine shrimp lethality bioassay and hemolytic activity were also evaluated. Significant differences were observed in the tests measured by NR uptake. The tumor cell lines TOV-21G and HepG2 and non-tumor WI-26VA4 cells showed relatively uniform toxicity results, with TOV-21G being the most sensitive cell tested, presenting the lowest concentration to cause death to 50% of viable cells (CC50) values. The results of this study support the use of TOV-21G, HepG2 and WI-26VA4 cells lines as the choice for cytotoxicity tests to evaluate potential bioactive compounds.info:eu-repo/semantics/openAccessUniversidade de São Paulo, Faculdade de Ciências FarmacêuticasBrazilian Journal of Pharmaceutical Sciences v.58 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100540en10.1590/s2175-97902022e18308 |
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Espíndola,Mariana Rodrigues Varotti,Fernando de Pilla Aguiar,Anna Caroline Campos Andrade,Silmara Nunes Rocha,Eliana Maria Mauricio da |
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Espíndola,Mariana Rodrigues Varotti,Fernando de Pilla Aguiar,Anna Caroline Campos Andrade,Silmara Nunes Rocha,Eliana Maria Mauricio da In vitro assessment for cytotoxicity screening of new antimalarial candidates |
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Espíndola,Mariana Rodrigues Varotti,Fernando de Pilla Aguiar,Anna Caroline Campos Andrade,Silmara Nunes Rocha,Eliana Maria Mauricio da |
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Espíndola,Mariana Rodrigues |
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In vitro assessment for cytotoxicity screening of new antimalarial candidates |
title_short |
In vitro assessment for cytotoxicity screening of new antimalarial candidates |
title_full |
In vitro assessment for cytotoxicity screening of new antimalarial candidates |
title_fullStr |
In vitro assessment for cytotoxicity screening of new antimalarial candidates |
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In vitro assessment for cytotoxicity screening of new antimalarial candidates |
title_sort |
in vitro assessment for cytotoxicity screening of new antimalarial candidates |
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Abstract In antimalarial research there are no standard procedures to determine the toxicity of a drug candidate. Among the alternatives available, in vitro cytotoxicity assays are the most widely used to predict toxic effects of future therapeutic products. They have the advantage over the in vivo assays, in that they offer the possibility to restrain the number of experimental variables. The objective of the present study was to compare in vitro cytotoxic methods by testing various compounds currently used to treat malaria against different cell lines. Neutral red (NR) uptake and methylthiazoletetrazolium (MTT) colorimetric in vitro assays were used to determine preliminary toxicity of commercially available antimalarial drugs against tumor and non-tumor cells lines. Toxicity through brine shrimp lethality bioassay and hemolytic activity were also evaluated. Significant differences were observed in the tests measured by NR uptake. The tumor cell lines TOV-21G and HepG2 and non-tumor WI-26VA4 cells showed relatively uniform toxicity results, with TOV-21G being the most sensitive cell tested, presenting the lowest concentration to cause death to 50% of viable cells (CC50) values. The results of this study support the use of TOV-21G, HepG2 and WI-26VA4 cells lines as the choice for cytotoxicity tests to evaluate potential bioactive compounds. |
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Universidade de São Paulo, Faculdade de Ciências Farmacêuticas |
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2022 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100540 |
work_keys_str_mv |
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