Assessment of coconut water added to Numida meleagris egg yolk as cryoprotectant for goat semen
Abstract Effects were assessed of the dilutants TRIS and ACP - 101c® with the addition of different guinea fowl (Numida meleagris) egg yolk concentrations. Fifteen ejaculates were collected from five goats of the Anglo Nubian breed. The ejaculates were pooled and then divided into 12 groups, two control groups (GC1 TRIS, with 2.5% Gallus gallus domesticus hen egg yolk GOGD), (GC2 Control Group ACP - 101c®, with the addition of 2.5% Gallus gallus domesticus hen egg yolk GOGD) and ten experimental groups (EG), containing TRIS and ACP added with different concentrations of egg yolk from guinea hen (Numida meleagris) (TRIS 2,5% GONM; TRIS 5% GONM; TRIS 10% GONM; TRIS 15% GONM; TRIS 20% GONM; ACP® 2,5% GONM; ACP® 5% GONM; ACP® 10% GONM; ACP® 15% GONM; ACP® 20% GONM). Then cryopreservation was carried out and the samples stored in liquid nitrogen (-196 °C). After seven days, the samples were thawed and assessed for spermatic kinetics, immunofluorescence and sperm morphology. Analysis of GOMN by the CASA system showed that the various parameters were similar to those of GOGD (P>0.05). The membrane integrity, mitochondrial potential and the acrosome were not influenced by the treatment (P>0.05) nor by the dilutant used for cryopreservation (P>0.05). The spermatic morphology was also preserved by the different GOGD and GONM concentrations in the ACP® and TRIS dilutants, with no statistically significant differences (P<0.05). It was concluded that Numida meleagris egg yolk, as external membrane cryoproctant added to the dilutants ACP-101c® and TRIS, improved goat semen quality.
Main Authors: | Macêdo,Laércio Fontinele Bandeira de, Texeira,Letícia Soares de Araújo, Braga,Clarissa de Castro e, Porfírio,Kenney de Paiva, Costa,Sara Camila da Silveira, Silva,Louis Henrique Miyauchi, Nascimento,Wcleuden Matias, Silva,Francisca Kelly dos Santos, Bandeira,Nildene Silva Andrade, Vieira,Rômulo José, Mineiro,Ana Lys Bezerra Barradas, Salgueiro,Cristiane Clemente de Mello, Nunes,José Ferreira, Cardoso,Janaina de Fátima Saraiva, Paula,Ney Rômulo de Oliveira |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Colégio Brasileiro de Reprodução Animal
2022
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-31432022000300206 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Heterakiosis en Numida meleagris (Aves: Numididae)
by: Mirope Santa Cruz, A.C., et al. -
Energy and protein levels in initial diets for guinea fowl (Numida meleagris)
by: Lopes, Irani Ribeiro Vieira, et al.
Published: (1996) -
Estudio del Os prefrontale en Numida meleagris (L.,1758)
by: Freitas Berbel, Juan Antonio 20731, et al.
Published: (1999) -
Performance of indigenous guinea fowls (numida meleagris) fed direct-fed microbial
by: Sarfo, G.K., et al.
Published: (2016-03-30) -
EFFECT OF EGGSHELL COLOR ON THE EGG CHARACTERISTICS AND HATCHABILITY OF GUINEA FOWL (NUMIDA MELEAGRIS) EGGS
by: Eleroğlu,H, et al.
Published: (2016)