Testicular morphometry of rats with Walker 256 tumor supplemented with L-glutamine
Abstract Glutamine is often used to treat metabolic changes associated with anorexia-cachexia syndrome in patients with malignant neoplasms. Walker 256 tumor is an excellent model for studying these changes associated with cancer in different organs, including injuries in testicular functions. However, the effects of supplementing glutamine on testicular morphometry in this model have not yet been investigated. Thus, the objective of this study was to evaluate the effect of L-glutamine supplementation on testicular morphometry in rats transplanted with Walker 256 tumor cells. Forty puberty Wistar rats were divided into four groups: control without L-glutamine (C); control supplemented with L-glutamine (CG); inoculated with Walker 256 tumor cells (WT) and inoculated with Walker 256 tumor cells and supplemented with L-glutamine (WTG). The testicles were removed, weighed, fixed in Bouin, and included in paraffin for histomorphometric analysis. Walker 256 tumor caused quantitative changes in the tubular and intertubular compartments and tunica albuginea, with reductions in the percentages of lumen and tunica albuginea, number of Sertoli cells per gram of testis; number of Leydig cells; percentage of blood vessels and connective tissue in intertubule. However, glutamine supplementation prevented part of these changes caused by the tumor, presenting mainly a protective effect on the tunica albuginea and percentage of blood and lymph vessels in the intertubule. These results indicate the potential of L-glutamine was able to recover for testicular dysfunction associated with cancer.
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Colégio Brasileiro de Reprodução Animal
2021
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oai:scielo:S1984-314320210002002062021-07-16Testicular morphometry of rats with Walker 256 tumor supplemented with L-glutamineRocha,Nayara RodriguesBraz,Janine Karla França da SilvaSouza,Sara Raquel Garcia deFracaro,LucianeMelo,Fabiana Cristina Silveira Alves deZanoni,Jacqueline NelisisClebis,Naianne KellyMorais,Danielle BarbosaMoura,Carlos Eduardo Bezerra de histomorphometry intertubule seminiferous tubules spermatogenesis Walker carcinoma Abstract Glutamine is often used to treat metabolic changes associated with anorexia-cachexia syndrome in patients with malignant neoplasms. Walker 256 tumor is an excellent model for studying these changes associated with cancer in different organs, including injuries in testicular functions. However, the effects of supplementing glutamine on testicular morphometry in this model have not yet been investigated. Thus, the objective of this study was to evaluate the effect of L-glutamine supplementation on testicular morphometry in rats transplanted with Walker 256 tumor cells. Forty puberty Wistar rats were divided into four groups: control without L-glutamine (C); control supplemented with L-glutamine (CG); inoculated with Walker 256 tumor cells (WT) and inoculated with Walker 256 tumor cells and supplemented with L-glutamine (WTG). The testicles were removed, weighed, fixed in Bouin, and included in paraffin for histomorphometric analysis. Walker 256 tumor caused quantitative changes in the tubular and intertubular compartments and tunica albuginea, with reductions in the percentages of lumen and tunica albuginea, number of Sertoli cells per gram of testis; number of Leydig cells; percentage of blood vessels and connective tissue in intertubule. However, glutamine supplementation prevented part of these changes caused by the tumor, presenting mainly a protective effect on the tunica albuginea and percentage of blood and lymph vessels in the intertubule. These results indicate the potential of L-glutamine was able to recover for testicular dysfunction associated with cancer.info:eu-repo/semantics/openAccessColégio Brasileiro de Reprodução AnimalAnimal Reproduction v.18 n.2 20212021-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-31432021000200206en10.1590/1984-3143-ar2020-0051 |
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Rocha,Nayara Rodrigues Braz,Janine Karla França da Silva Souza,Sara Raquel Garcia de Fracaro,Luciane Melo,Fabiana Cristina Silveira Alves de Zanoni,Jacqueline Nelisis Clebis,Naianne Kelly Morais,Danielle Barbosa Moura,Carlos Eduardo Bezerra de |
| spellingShingle |
Rocha,Nayara Rodrigues Braz,Janine Karla França da Silva Souza,Sara Raquel Garcia de Fracaro,Luciane Melo,Fabiana Cristina Silveira Alves de Zanoni,Jacqueline Nelisis Clebis,Naianne Kelly Morais,Danielle Barbosa Moura,Carlos Eduardo Bezerra de Testicular morphometry of rats with Walker 256 tumor supplemented with L-glutamine |
| author_facet |
Rocha,Nayara Rodrigues Braz,Janine Karla França da Silva Souza,Sara Raquel Garcia de Fracaro,Luciane Melo,Fabiana Cristina Silveira Alves de Zanoni,Jacqueline Nelisis Clebis,Naianne Kelly Morais,Danielle Barbosa Moura,Carlos Eduardo Bezerra de |
| author_sort |
Rocha,Nayara Rodrigues |
| title |
Testicular morphometry of rats with Walker 256 tumor supplemented with L-glutamine |
| title_short |
Testicular morphometry of rats with Walker 256 tumor supplemented with L-glutamine |
| title_full |
Testicular morphometry of rats with Walker 256 tumor supplemented with L-glutamine |
| title_fullStr |
Testicular morphometry of rats with Walker 256 tumor supplemented with L-glutamine |
| title_full_unstemmed |
Testicular morphometry of rats with Walker 256 tumor supplemented with L-glutamine |
| title_sort |
testicular morphometry of rats with walker 256 tumor supplemented with l-glutamine |
| description |
Abstract Glutamine is often used to treat metabolic changes associated with anorexia-cachexia syndrome in patients with malignant neoplasms. Walker 256 tumor is an excellent model for studying these changes associated with cancer in different organs, including injuries in testicular functions. However, the effects of supplementing glutamine on testicular morphometry in this model have not yet been investigated. Thus, the objective of this study was to evaluate the effect of L-glutamine supplementation on testicular morphometry in rats transplanted with Walker 256 tumor cells. Forty puberty Wistar rats were divided into four groups: control without L-glutamine (C); control supplemented with L-glutamine (CG); inoculated with Walker 256 tumor cells (WT) and inoculated with Walker 256 tumor cells and supplemented with L-glutamine (WTG). The testicles were removed, weighed, fixed in Bouin, and included in paraffin for histomorphometric analysis. Walker 256 tumor caused quantitative changes in the tubular and intertubular compartments and tunica albuginea, with reductions in the percentages of lumen and tunica albuginea, number of Sertoli cells per gram of testis; number of Leydig cells; percentage of blood vessels and connective tissue in intertubule. However, glutamine supplementation prevented part of these changes caused by the tumor, presenting mainly a protective effect on the tunica albuginea and percentage of blood and lymph vessels in the intertubule. These results indicate the potential of L-glutamine was able to recover for testicular dysfunction associated with cancer. |
| publisher |
Colégio Brasileiro de Reprodução Animal |
| publishDate |
2021 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-31432021000200206 |
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