Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia
Abstract Small non-volant mammals (marsupials and small rodents) were captured at three different timepoints from 23 forest fragments across three municipalities (Alta Floresta, Sinop and Cláudia) covering the Amazonian biome of the Mato Grosso State in Midwestern Brazil. The animal tissues (liver and spleen) and blood were screened using molecular tools for the detection of Babesia, Coxiella, Cytauxzoon, Hepatozoon, Theileria, and Anaplasmataceae agents. A total of 230 specimens (78 rodents and 152 marsupials) were trapped. Hepatozoon and Piroplasmorida agents were detected in the common opossums (Didelphis marsupialis). In turn, all samples (blood, liver, or spleen) collected from the small mammals were negative for the genus Coxiella and the family Anaplasmataceae, as detected by polymerase chain reaction (PCR). Phylogenetic analyses inferred from partial sequences of the 18S rRNA gene highlighted the occurrence of new Hepatozoon and Piroplasmorida haplotypes. Future studies determining the role of common opossum (D. marsupialis) in the epidemiological cycles of Hepatozoon and Babesia under natural conditions in the Amazonian biome are necessary.
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Colégio Brasileiro de Parasitologia Veterinária
2019
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oai:scielo:S1984-296120190004005922020-04-14Molecular survey of tick-borne pathogens in small mammals from Brazilian AmazoniaColle,Ana CláudiaMendonça,Ravena Fernanda Braga deMaia,Maerle OliveiraFreitas,Leodil da CostaWitter,RuteMarcili,ArleiAguiar,Daniel Moura deMuñoz-Leal,SebastiánLabruna,Marcelo BahiaRossi,Rogério VieiraPacheco,Richard de Campos Hepatozoon Babesia Amazonian biome Mato Grosso state Abstract Small non-volant mammals (marsupials and small rodents) were captured at three different timepoints from 23 forest fragments across three municipalities (Alta Floresta, Sinop and Cláudia) covering the Amazonian biome of the Mato Grosso State in Midwestern Brazil. The animal tissues (liver and spleen) and blood were screened using molecular tools for the detection of Babesia, Coxiella, Cytauxzoon, Hepatozoon, Theileria, and Anaplasmataceae agents. A total of 230 specimens (78 rodents and 152 marsupials) were trapped. Hepatozoon and Piroplasmorida agents were detected in the common opossums (Didelphis marsupialis). In turn, all samples (blood, liver, or spleen) collected from the small mammals were negative for the genus Coxiella and the family Anaplasmataceae, as detected by polymerase chain reaction (PCR). Phylogenetic analyses inferred from partial sequences of the 18S rRNA gene highlighted the occurrence of new Hepatozoon and Piroplasmorida haplotypes. Future studies determining the role of common opossum (D. marsupialis) in the epidemiological cycles of Hepatozoon and Babesia under natural conditions in the Amazonian biome are necessary.info:eu-repo/semantics/openAccessColégio Brasileiro de Parasitologia VeterináriaRevista Brasileira de Parasitologia Veterinária v.28 n.4 20192019-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000400592en10.1590/s1984-29612019086 |
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Colle,Ana Cláudia Mendonça,Ravena Fernanda Braga de Maia,Maerle Oliveira Freitas,Leodil da Costa Witter,Rute Marcili,Arlei Aguiar,Daniel Moura de Muñoz-Leal,Sebastián Labruna,Marcelo Bahia Rossi,Rogério Vieira Pacheco,Richard de Campos |
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Colle,Ana Cláudia Mendonça,Ravena Fernanda Braga de Maia,Maerle Oliveira Freitas,Leodil da Costa Witter,Rute Marcili,Arlei Aguiar,Daniel Moura de Muñoz-Leal,Sebastián Labruna,Marcelo Bahia Rossi,Rogério Vieira Pacheco,Richard de Campos Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia |
author_facet |
Colle,Ana Cláudia Mendonça,Ravena Fernanda Braga de Maia,Maerle Oliveira Freitas,Leodil da Costa Witter,Rute Marcili,Arlei Aguiar,Daniel Moura de Muñoz-Leal,Sebastián Labruna,Marcelo Bahia Rossi,Rogério Vieira Pacheco,Richard de Campos |
author_sort |
Colle,Ana Cláudia |
title |
Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia |
title_short |
Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia |
title_full |
Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia |
title_fullStr |
Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia |
title_full_unstemmed |
Molecular survey of tick-borne pathogens in small mammals from Brazilian Amazonia |
title_sort |
molecular survey of tick-borne pathogens in small mammals from brazilian amazonia |
description |
Abstract Small non-volant mammals (marsupials and small rodents) were captured at three different timepoints from 23 forest fragments across three municipalities (Alta Floresta, Sinop and Cláudia) covering the Amazonian biome of the Mato Grosso State in Midwestern Brazil. The animal tissues (liver and spleen) and blood were screened using molecular tools for the detection of Babesia, Coxiella, Cytauxzoon, Hepatozoon, Theileria, and Anaplasmataceae agents. A total of 230 specimens (78 rodents and 152 marsupials) were trapped. Hepatozoon and Piroplasmorida agents were detected in the common opossums (Didelphis marsupialis). In turn, all samples (blood, liver, or spleen) collected from the small mammals were negative for the genus Coxiella and the family Anaplasmataceae, as detected by polymerase chain reaction (PCR). Phylogenetic analyses inferred from partial sequences of the 18S rRNA gene highlighted the occurrence of new Hepatozoon and Piroplasmorida haplotypes. Future studies determining the role of common opossum (D. marsupialis) in the epidemiological cycles of Hepatozoon and Babesia under natural conditions in the Amazonian biome are necessary. |
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Colégio Brasileiro de Parasitologia Veterinária |
publishDate |
2019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000400592 |
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