Molecular detection of Leishmania spp. in cattle from Brazil by means of PCR using internal transcribed spacer 1

Molecular detection of Leishmania spp. in cattle from Brazil by means of PCR using internal transcribed spacer 1

Abstract Leishmania spp. are important agents of human and animal leishmaniases that have an important impact on public health. In this study, we aimed to detect the circulation of Leishmania spp. in cattle from a visceral leishmaniasis non-endemic area of the state of São Paulo, Brazil. DNA was extracted from blood samples from 100 heifers in the municipality of Pirassununga and was amplified using primers specific for the first internal transcriber spacer (ITS1), to assess the presence of trypanosomatids. The assays revealed that one sample presented bands of between 300 and 350 base pairs. In GenBank, this sample matched 100% with Leishmania infantum (314 base pairs). The results suggest that cattle can be infected by Leishmania infantum in Brazil.

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Main Authors: Vioti,Geovanna, Leonel,João Augusto Franco, Lemes,Kleber Menegon, Pereira,Vanessa Figueredo, Ferreira,Helena Lage, Keid,Lara Borges, Madureira,Ed Hoffman, Soares,Rodrigo Martins, Benassi,Júlia Cristina, Oliveira,Trícia Maria Ferreira de Sousa
Format: Digital revista
Language:English
Published: Colégio Brasileiro de Parasitologia Veterinária 2019
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000200303
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spelling oai:scielo:S1984-296120190002003032019-06-18Molecular detection of Leishmania spp. in cattle from Brazil by means of PCR using internal transcribed spacer 1Vioti,GeovannaLeonel,João Augusto FrancoLemes,Kleber MenegonPereira,Vanessa FigueredoFerreira,Helena LageKeid,Lara BorgesMadureira,Ed HoffmanSoares,Rodrigo MartinsBenassi,Júlia CristinaOliveira,Trícia Maria Ferreira de Sousa Cattle ITS1 Leishmania infantum São Paulo Abstract Leishmania spp. are important agents of human and animal leishmaniases that have an important impact on public health. In this study, we aimed to detect the circulation of Leishmania spp. in cattle from a visceral leishmaniasis non-endemic area of the state of São Paulo, Brazil. DNA was extracted from blood samples from 100 heifers in the municipality of Pirassununga and was amplified using primers specific for the first internal transcriber spacer (ITS1), to assess the presence of trypanosomatids. The assays revealed that one sample presented bands of between 300 and 350 base pairs. In GenBank, this sample matched 100% with Leishmania infantum (314 base pairs). The results suggest that cattle can be infected by Leishmania infantum in Brazil.info:eu-repo/semantics/openAccessColégio Brasileiro de Parasitologia VeterináriaRevista Brasileira de Parasitologia Veterinária v.28 n.2 20192019-04-01info:eu-repo/semantics/reporttext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000200303en10.1590/s1984-29612019003
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Vioti,Geovanna
Leonel,João Augusto Franco
Lemes,Kleber Menegon
Pereira,Vanessa Figueredo
Ferreira,Helena Lage
Keid,Lara Borges
Madureira,Ed Hoffman
Soares,Rodrigo Martins
Benassi,Júlia Cristina
Oliveira,Trícia Maria Ferreira de Sousa
spellingShingle Vioti,Geovanna
Leonel,João Augusto Franco
Lemes,Kleber Menegon
Pereira,Vanessa Figueredo
Ferreira,Helena Lage
Keid,Lara Borges
Madureira,Ed Hoffman
Soares,Rodrigo Martins
Benassi,Júlia Cristina
Oliveira,Trícia Maria Ferreira de Sousa
Molecular detection of Leishmania spp. in cattle from Brazil by means of PCR using internal transcribed spacer 1
author_facet Vioti,Geovanna
Leonel,João Augusto Franco
Lemes,Kleber Menegon
Pereira,Vanessa Figueredo
Ferreira,Helena Lage
Keid,Lara Borges
Madureira,Ed Hoffman
Soares,Rodrigo Martins
Benassi,Júlia Cristina
Oliveira,Trícia Maria Ferreira de Sousa
author_sort Vioti,Geovanna
title Molecular detection of Leishmania spp. in cattle from Brazil by means of PCR using internal transcribed spacer 1
title_short Molecular detection of Leishmania spp. in cattle from Brazil by means of PCR using internal transcribed spacer 1
title_full Molecular detection of Leishmania spp. in cattle from Brazil by means of PCR using internal transcribed spacer 1
title_fullStr Molecular detection of Leishmania spp. in cattle from Brazil by means of PCR using internal transcribed spacer 1
title_full_unstemmed Molecular detection of Leishmania spp. in cattle from Brazil by means of PCR using internal transcribed spacer 1
title_sort molecular detection of leishmania spp. in cattle from brazil by means of pcr using internal transcribed spacer 1
description Abstract Leishmania spp. are important agents of human and animal leishmaniases that have an important impact on public health. In this study, we aimed to detect the circulation of Leishmania spp. in cattle from a visceral leishmaniasis non-endemic area of the state of São Paulo, Brazil. DNA was extracted from blood samples from 100 heifers in the municipality of Pirassununga and was amplified using primers specific for the first internal transcriber spacer (ITS1), to assess the presence of trypanosomatids. The assays revealed that one sample presented bands of between 300 and 350 base pairs. In GenBank, this sample matched 100% with Leishmania infantum (314 base pairs). The results suggest that cattle can be infected by Leishmania infantum in Brazil.
publisher Colégio Brasileiro de Parasitologia Veterinária
publishDate 2019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000200303
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