In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses
Abstract Cyathostomins are the most prevalent nematodes of horses, and multidrug resistance has been reported worldwide. There is a need to implement alternative drug monitoring analytical tests. The objective of this study was to determine the consistency (5 repetitions) of the larval migration on agar test (LMAT) using ivermectin, moxidectin, pyrantel or albendazole against cyathostomin infective-stage larvae in eight different concentrations. LMAT showed a strong coefficient of determination (R2 > 0.91), between the test repetitions (n=5). The average 50% effective concentration (EC50) for ivermectin, moxidectin, pyrantel and albendazole were 0.0404, 0.0558, 0.0864 and 0.0988 nMol, respectively. The results of the EC50 for albendazole showed the greatest range of concentration. Ivermectin and moxidectin had the lowest in between-test variation. In the future, internationally certified susceptible isolates could be used for screening new drug candidates, or to follow up the pattern of drug efficacy from field populations.
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Colégio Brasileiro de Parasitologia Veterinária
2018
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oai:scielo:S1984-296120180001000902018-04-17In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horsesMolento,Marcelo BeltrãoCanever,Ricardo José Horse Anthelmintics In vitro test parasites Abstract Cyathostomins are the most prevalent nematodes of horses, and multidrug resistance has been reported worldwide. There is a need to implement alternative drug monitoring analytical tests. The objective of this study was to determine the consistency (5 repetitions) of the larval migration on agar test (LMAT) using ivermectin, moxidectin, pyrantel or albendazole against cyathostomin infective-stage larvae in eight different concentrations. LMAT showed a strong coefficient of determination (R2 > 0.91), between the test repetitions (n=5). The average 50% effective concentration (EC50) for ivermectin, moxidectin, pyrantel and albendazole were 0.0404, 0.0558, 0.0864 and 0.0988 nMol, respectively. The results of the EC50 for albendazole showed the greatest range of concentration. Ivermectin and moxidectin had the lowest in between-test variation. In the future, internationally certified susceptible isolates could be used for screening new drug candidates, or to follow up the pattern of drug efficacy from field populations.info:eu-repo/semantics/openAccessColégio Brasileiro de Parasitologia VeterináriaRevista Brasileira de Parasitologia Veterinária v.27 n.1 20182018-03-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000100090en10.1590/s1984-29612017055 |
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Molento,Marcelo Beltrão Canever,Ricardo José |
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Molento,Marcelo Beltrão Canever,Ricardo José In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses |
| author_facet |
Molento,Marcelo Beltrão Canever,Ricardo José |
| author_sort |
Molento,Marcelo Beltrão |
| title |
In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses |
| title_short |
In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses |
| title_full |
In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses |
| title_fullStr |
In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses |
| title_full_unstemmed |
In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses |
| title_sort |
in vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses |
| description |
Abstract Cyathostomins are the most prevalent nematodes of horses, and multidrug resistance has been reported worldwide. There is a need to implement alternative drug monitoring analytical tests. The objective of this study was to determine the consistency (5 repetitions) of the larval migration on agar test (LMAT) using ivermectin, moxidectin, pyrantel or albendazole against cyathostomin infective-stage larvae in eight different concentrations. LMAT showed a strong coefficient of determination (R2 > 0.91), between the test repetitions (n=5). The average 50% effective concentration (EC50) for ivermectin, moxidectin, pyrantel and albendazole were 0.0404, 0.0558, 0.0864 and 0.0988 nMol, respectively. The results of the EC50 for albendazole showed the greatest range of concentration. Ivermectin and moxidectin had the lowest in between-test variation. In the future, internationally certified susceptible isolates could be used for screening new drug candidates, or to follow up the pattern of drug efficacy from field populations. |
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Colégio Brasileiro de Parasitologia Veterinária |
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2018 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000100090 |
| work_keys_str_mv |
AT molentomarcelobeltrao invitroevaluationofivermectinmoxidectinalbendazoleandpyrantelagainstcyathostominsofhorses AT caneverricardojose invitroevaluationofivermectinmoxidectinalbendazoleandpyrantelagainstcyathostominsofhorses |
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