In vitro efficacy of Duddingtonia flagrans against nematodes of sheep based on in vivo calculations
Abstract Duddingtonia flagrans has been tested as an alternative parasite control, but data from in vitro experiments based on in vivo calculations describing nematophagous fungi predation in nematodes are restricted. The objective of this work was to determine the efficacy of D. flagrans against sheep nematode larvae in vitro using in vivo calculations. Fecal samples were introduced to fungi in different concentrations: 0.0/control; 0.05; 0.1; 0.2; 0.4; 0.8; 1.6; 3.2; and 6.4 g corresponding, respectively, to 583.000; 1.166.000; 2.332.000; 4.664.000; 9.328.000; 18.656.000; 37.312.000 and 74.624.000 chlamydospores/kg of body weight. The material was incubated for 14 days, before the larvae recovery (Assay 1). Assay 2 was carried out with the doses of 0.00625; 0.0125; and 0.025 g. The results showed a negative correlation between fungal concentrations and larval numbers for both assays. The fungus demonstrated an efficacy above 89% in both assays. Thus, we consider that the data from in vitro studies based on in vivo calculations may optimize the fungi quantities for field experiments.
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Colégio Brasileiro de Parasitologia Veterinária
2018
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oai:scielo:S1984-296120180001000862018-04-17In vitro efficacy of Duddingtonia flagrans against nematodes of sheep based on in vivo calculationsMolento,Marcelo BeltrãoAraújo,Flávia Biasoli deBuzatti,AndréiaSantos,Clóvis de Paula Duddingtonia flagrans predatory action larvae sheep gastrointestinal nematodes Abstract Duddingtonia flagrans has been tested as an alternative parasite control, but data from in vitro experiments based on in vivo calculations describing nematophagous fungi predation in nematodes are restricted. The objective of this work was to determine the efficacy of D. flagrans against sheep nematode larvae in vitro using in vivo calculations. Fecal samples were introduced to fungi in different concentrations: 0.0/control; 0.05; 0.1; 0.2; 0.4; 0.8; 1.6; 3.2; and 6.4 g corresponding, respectively, to 583.000; 1.166.000; 2.332.000; 4.664.000; 9.328.000; 18.656.000; 37.312.000 and 74.624.000 chlamydospores/kg of body weight. The material was incubated for 14 days, before the larvae recovery (Assay 1). Assay 2 was carried out with the doses of 0.00625; 0.0125; and 0.025 g. The results showed a negative correlation between fungal concentrations and larval numbers for both assays. The fungus demonstrated an efficacy above 89% in both assays. Thus, we consider that the data from in vitro studies based on in vivo calculations may optimize the fungi quantities for field experiments.info:eu-repo/semantics/openAccessColégio Brasileiro de Parasitologia VeterináriaRevista Brasileira de Parasitologia Veterinária v.27 n.1 20182018-03-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000100086en10.1590/s1984-29612017050 |
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Molento,Marcelo Beltrão Araújo,Flávia Biasoli de Buzatti,Andréia Santos,Clóvis de Paula |
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Molento,Marcelo Beltrão Araújo,Flávia Biasoli de Buzatti,Andréia Santos,Clóvis de Paula In vitro efficacy of Duddingtonia flagrans against nematodes of sheep based on in vivo calculations |
| author_facet |
Molento,Marcelo Beltrão Araújo,Flávia Biasoli de Buzatti,Andréia Santos,Clóvis de Paula |
| author_sort |
Molento,Marcelo Beltrão |
| title |
In vitro efficacy of Duddingtonia flagrans against nematodes of sheep based on in vivo calculations |
| title_short |
In vitro efficacy of Duddingtonia flagrans against nematodes of sheep based on in vivo calculations |
| title_full |
In vitro efficacy of Duddingtonia flagrans against nematodes of sheep based on in vivo calculations |
| title_fullStr |
In vitro efficacy of Duddingtonia flagrans against nematodes of sheep based on in vivo calculations |
| title_full_unstemmed |
In vitro efficacy of Duddingtonia flagrans against nematodes of sheep based on in vivo calculations |
| title_sort |
in vitro efficacy of duddingtonia flagrans against nematodes of sheep based on in vivo calculations |
| description |
Abstract Duddingtonia flagrans has been tested as an alternative parasite control, but data from in vitro experiments based on in vivo calculations describing nematophagous fungi predation in nematodes are restricted. The objective of this work was to determine the efficacy of D. flagrans against sheep nematode larvae in vitro using in vivo calculations. Fecal samples were introduced to fungi in different concentrations: 0.0/control; 0.05; 0.1; 0.2; 0.4; 0.8; 1.6; 3.2; and 6.4 g corresponding, respectively, to 583.000; 1.166.000; 2.332.000; 4.664.000; 9.328.000; 18.656.000; 37.312.000 and 74.624.000 chlamydospores/kg of body weight. The material was incubated for 14 days, before the larvae recovery (Assay 1). Assay 2 was carried out with the doses of 0.00625; 0.0125; and 0.025 g. The results showed a negative correlation between fungal concentrations and larval numbers for both assays. The fungus demonstrated an efficacy above 89% in both assays. Thus, we consider that the data from in vitro studies based on in vivo calculations may optimize the fungi quantities for field experiments. |
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Colégio Brasileiro de Parasitologia Veterinária |
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2018 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000100086 |
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