Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds
Abstract The anthelminthic activity of the essential oil (EO) of Piper aduncum L. was tested in vitro on eggs and larvae of resistant (Embrapa2010) and susceptible (McMaster) isolates of Haemonchus contortus. The EO was obtained by steam distillation and its components identified by chromatography. EO concentrations of 12.5 to 0.02 mg/mL were used in the egg hatch test (EHT) and concentrations of 3.12 to 0.01 mg/mL in the larval development test (LDT). Inhibition concentrations (IC) were determined by the SAS Probit procedure, and significant differences assessed by ANOVA followed by Tukey’s test. In the EHT, the IC50 for the susceptible isolate was 5.72 mg/mL. In the LDT, the IC50 and IC90 were, respectively, 0.10 mg/mL and 0.34 mg/mL for the susceptible isolate, and 0.22 mg/mL and 0.51 mg/mL for the resistant isolate. The EO (dillapiole 76.2%) was highly efficacious on phase L1. Due to the higher ICs obtained for the resistant isolate, it was raised the hypothesis that dillapiole may have a mechanism of action that resembles those of other anthelmintic compounds. We further review and discuss studies, especially those conducted in Brazil, that quantified the major constituents of P. aduncum-derived EO.
Main Authors: | Gaínza,Yousmel Alemán, Fantatto,Rafaela Regina, Chaves,Francisco Celio Maia, Bizzo,Humberto Ribeiro, Esteves,Sérgio Novita, Chagas,Ana Carolina de Souza |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Colégio Brasileiro de Parasitologia Veterinária
2016
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000400383 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds.
by: GAÍNZA, Y. A., et al.
Published: (2016-12-15) -
In vitro activity of the essential oil from Hesperozygis myrtoides on Rhipicephalus (Boophilus) microplus and Haemonchus contortus
by: Castilho,Caroline V.V., et al.
Published: (2017) -
Anthelmintic resistance of Haemonchus contortus from sheep flocks in Brazil: concordance of in vivo and in vitro (RESISTA-Test©) methods
by: Gainza,Yousmel Alemán, et al.
Published: (2021) -
Chemical composition and efficacy in the egg-hatching inhibition of essential oil of Piper aduncum against Haemonchus contortus from sheep
by: Oliveira,Gisele Lopes, et al.
Published: (2014) -
In vitro culture of parasitic stages of Haemonchus contortus
by: Niciura,Simone Cristina Méo, et al.
Published: (2023)