Conjunctival swab PCR to detect Leishmania spp. in cats

The relevance of the dog as a source of visceral leishmaniasis infection is known, but the role of cats as reservoir hosts for leishmaniasis is not yet fully clear. This study assessed the efficacy of conjunctival swab PCR (CS-PCR) in the detection of cats infected by Leishmania spp. The results were seven (13.5%) cats positive for Leishmania spp. in the PCR, in 52 cats tested from Pirassunuga-SP and Ilha Solteira-SP. From the city of Pirassununga – SP 28.6% (2/7) were positive and from the city of Ilha Solteira – SP 11.1% (5/45) were positive. The results showed that CS-PCR was capable of detecting cats infected by this protozoan. Conjunctival swab samples proved easier to perform in cats, which might facilitate studies on the frequency and distribution of feline leishmaniasis.

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Main Authors: Oliveira,Trícia Maria Ferreira de Sousa, Pereira,Vanessa Figueredo, Benvenga,Graziella Ulbricht, Martin,Maria Fernanda Alves, Benassi,Julia Cristina, Silva,Diogo Tiago da, Starke-Buzetti,Wilma Aparecida
Format: Digital revista
Language:English
Published: Colégio Brasileiro de Parasitologia Veterinária 2015
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612015000200220
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spelling oai:scielo:S1984-296120150002002202015-10-09Conjunctival swab PCR to detect Leishmania spp. in catsOliveira,Trícia Maria Ferreira de SousaPereira,Vanessa FigueredoBenvenga,Graziella UlbrichtMartin,Maria Fernanda AlvesBenassi,Julia CristinaSilva,Diogo Tiago daStarke-Buzetti,Wilma Aparecida Conjunctival swab PCR cats The relevance of the dog as a source of visceral leishmaniasis infection is known, but the role of cats as reservoir hosts for leishmaniasis is not yet fully clear. This study assessed the efficacy of conjunctival swab PCR (CS-PCR) in the detection of cats infected by Leishmania spp. The results were seven (13.5%) cats positive for Leishmania spp. in the PCR, in 52 cats tested from Pirassunuga-SP and Ilha Solteira-SP. From the city of Pirassununga – SP 28.6% (2/7) were positive and from the city of Ilha Solteira – SP 11.1% (5/45) were positive. The results showed that CS-PCR was capable of detecting cats infected by this protozoan. Conjunctival swab samples proved easier to perform in cats, which might facilitate studies on the frequency and distribution of feline leishmaniasis.info:eu-repo/semantics/openAccessColégio Brasileiro de Parasitologia VeterináriaRevista Brasileira de Parasitologia Veterinária v.24 n.2 20152015-06-01info:eu-repo/semantics/reporttext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612015000200220en10.1590/S1984-29612015016
institution SCIELO
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country Brasil
countrycode BR
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access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Oliveira,Trícia Maria Ferreira de Sousa
Pereira,Vanessa Figueredo
Benvenga,Graziella Ulbricht
Martin,Maria Fernanda Alves
Benassi,Julia Cristina
Silva,Diogo Tiago da
Starke-Buzetti,Wilma Aparecida
spellingShingle Oliveira,Trícia Maria Ferreira de Sousa
Pereira,Vanessa Figueredo
Benvenga,Graziella Ulbricht
Martin,Maria Fernanda Alves
Benassi,Julia Cristina
Silva,Diogo Tiago da
Starke-Buzetti,Wilma Aparecida
Conjunctival swab PCR to detect Leishmania spp. in cats
author_facet Oliveira,Trícia Maria Ferreira de Sousa
Pereira,Vanessa Figueredo
Benvenga,Graziella Ulbricht
Martin,Maria Fernanda Alves
Benassi,Julia Cristina
Silva,Diogo Tiago da
Starke-Buzetti,Wilma Aparecida
author_sort Oliveira,Trícia Maria Ferreira de Sousa
title Conjunctival swab PCR to detect Leishmania spp. in cats
title_short Conjunctival swab PCR to detect Leishmania spp. in cats
title_full Conjunctival swab PCR to detect Leishmania spp. in cats
title_fullStr Conjunctival swab PCR to detect Leishmania spp. in cats
title_full_unstemmed Conjunctival swab PCR to detect Leishmania spp. in cats
title_sort conjunctival swab pcr to detect leishmania spp. in cats
description The relevance of the dog as a source of visceral leishmaniasis infection is known, but the role of cats as reservoir hosts for leishmaniasis is not yet fully clear. This study assessed the efficacy of conjunctival swab PCR (CS-PCR) in the detection of cats infected by Leishmania spp. The results were seven (13.5%) cats positive for Leishmania spp. in the PCR, in 52 cats tested from Pirassunuga-SP and Ilha Solteira-SP. From the city of Pirassununga – SP 28.6% (2/7) were positive and from the city of Ilha Solteira – SP 11.1% (5/45) were positive. The results showed that CS-PCR was capable of detecting cats infected by this protozoan. Conjunctival swab samples proved easier to perform in cats, which might facilitate studies on the frequency and distribution of feline leishmaniasis.
publisher Colégio Brasileiro de Parasitologia Veterinária
publishDate 2015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612015000200220
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