Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-α in leukocytes and splenocytes of experimentally infected dogs
Canine ehrlichiosis is caused by the bacterium Ehrlichia canis and is characterized by a systemic febrile disease of unknown pathogenesis. This study evaluated the expression of cytokines TNF-α, IL-10, IFN-γ, in splenic cells and blood leukocytes during the acute phase of ehrlichiosis and after treatment with doxycycline hyclate in dogs experimentally infected with the E. canis Jaboticabal strain. The study results showed a significant expression of TNF-α 18 days post-inoculation, reducing by approximately 70% after treatment. There was a unique peak of expression of IL-10 and IFN-γ 18 and 30 days post-inoculation, respectively. This study suggests that TNF-α plays a role in the pathogenesis of the acute phase of canine ehrlichiosis and that treatment with doxycycline hyclate reduces the systemic effects of this cytokine, possibly by reducing or eliminating parasitemia.
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Colégio Brasileiro de Parasitologia Veterinária
2011
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oai:scielo:S1984-296120110001000152011-04-20Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-α in leukocytes and splenocytes of experimentally infected dogsFaria,Joice Lara MaiaMunhoz,Thiago DemarchiJoão,Carolina FranchiVargas-Hernández,GiovannyAndré,Marcos RogérioPereira,Wanderson Adriano BiscolaMachado,Rosângela ZacariasTinucci-Costa,Mirela Ehrlichiosis dogs experimental infection IL-10 IFN-γ Canine ehrlichiosis is caused by the bacterium Ehrlichia canis and is characterized by a systemic febrile disease of unknown pathogenesis. This study evaluated the expression of cytokines TNF-α, IL-10, IFN-γ, in splenic cells and blood leukocytes during the acute phase of ehrlichiosis and after treatment with doxycycline hyclate in dogs experimentally infected with the E. canis Jaboticabal strain. The study results showed a significant expression of TNF-α 18 days post-inoculation, reducing by approximately 70% after treatment. There was a unique peak of expression of IL-10 and IFN-γ 18 and 30 days post-inoculation, respectively. This study suggests that TNF-α plays a role in the pathogenesis of the acute phase of canine ehrlichiosis and that treatment with doxycycline hyclate reduces the systemic effects of this cytokine, possibly by reducing or eliminating parasitemia.info:eu-repo/semantics/openAccessColégio Brasileiro de Parasitologia VeterináriaRevista Brasileira de Parasitologia Veterinária v.20 n.1 20112011-03-01info:eu-repo/semantics/reporttext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612011000100015en10.1590/S1984-29612011000100015 |
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Faria,Joice Lara Maia Munhoz,Thiago Demarchi João,Carolina Franchi Vargas-Hernández,Giovanny André,Marcos Rogério Pereira,Wanderson Adriano Biscola Machado,Rosângela Zacarias Tinucci-Costa,Mirela |
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Faria,Joice Lara Maia Munhoz,Thiago Demarchi João,Carolina Franchi Vargas-Hernández,Giovanny André,Marcos Rogério Pereira,Wanderson Adriano Biscola Machado,Rosângela Zacarias Tinucci-Costa,Mirela Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-α in leukocytes and splenocytes of experimentally infected dogs |
author_facet |
Faria,Joice Lara Maia Munhoz,Thiago Demarchi João,Carolina Franchi Vargas-Hernández,Giovanny André,Marcos Rogério Pereira,Wanderson Adriano Biscola Machado,Rosângela Zacarias Tinucci-Costa,Mirela |
author_sort |
Faria,Joice Lara Maia |
title |
Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-α in leukocytes and splenocytes of experimentally infected dogs |
title_short |
Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-α in leukocytes and splenocytes of experimentally infected dogs |
title_full |
Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-α in leukocytes and splenocytes of experimentally infected dogs |
title_fullStr |
Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-α in leukocytes and splenocytes of experimentally infected dogs |
title_full_unstemmed |
Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-α in leukocytes and splenocytes of experimentally infected dogs |
title_sort |
ehrlichia canis (jaboticabal strain) induces the expression of tnf-α in leukocytes and splenocytes of experimentally infected dogs |
description |
Canine ehrlichiosis is caused by the bacterium Ehrlichia canis and is characterized by a systemic febrile disease of unknown pathogenesis. This study evaluated the expression of cytokines TNF-α, IL-10, IFN-γ, in splenic cells and blood leukocytes during the acute phase of ehrlichiosis and after treatment with doxycycline hyclate in dogs experimentally infected with the E. canis Jaboticabal strain. The study results showed a significant expression of TNF-α 18 days post-inoculation, reducing by approximately 70% after treatment. There was a unique peak of expression of IL-10 and IFN-γ 18 and 30 days post-inoculation, respectively. This study suggests that TNF-α plays a role in the pathogenesis of the acute phase of canine ehrlichiosis and that treatment with doxycycline hyclate reduces the systemic effects of this cytokine, possibly by reducing or eliminating parasitemia. |
publisher |
Colégio Brasileiro de Parasitologia Veterinária |
publishDate |
2011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612011000100015 |
work_keys_str_mv |
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1756437041076240384 |