Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil
Blood and serum samples from 170 horses raised in the Jaboticabal microregion, São Paulo State, Brazil, were collected and tested by microscopic examination of blood smears, indirect fluorescent antibody test (IFAT) and nested polymerase chain reaction (nPCR) for Theileria equi infections. The association among the test results was verified by the McNemar test. During the examination of thin blood smears, parasites were detected in six (3.52%) horses. Anti-T. equi antibodies were detected in 100% sera samples, with titers ranging between 1:80 and 1:5120. The nPCR based on the T. equi merozoite antigen gene (EMA-1) allowed the visualization of specie-specific amplified product in 108 (63.53%) horses. All six samples judged positive microscopically were also positive for nPCR. Statistical analysis indicated general disagreement (p < 0.0001) between IFAT and nPCR; IFAT and blood smear; and nPCR and blood smear on the detection of parasite carriers. The results of the present study indicate that T. equi is widely spread among horses in the Jaboticabal microregion, Northeast region of São Paulo State, Brazil.
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Colégio Brasileiro de Parasitologia Veterinária
2010
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oai:scielo:S1984-296120100004000072011-02-07Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, BrazilBaldani,Cristiane DivanNakaghi,Andrea Cristina HigaMachado,Rosangela Zacarias Theileria equi horse diagnosis IFAT nested PCR Blood and serum samples from 170 horses raised in the Jaboticabal microregion, São Paulo State, Brazil, were collected and tested by microscopic examination of blood smears, indirect fluorescent antibody test (IFAT) and nested polymerase chain reaction (nPCR) for Theileria equi infections. The association among the test results was verified by the McNemar test. During the examination of thin blood smears, parasites were detected in six (3.52%) horses. Anti-T. equi antibodies were detected in 100% sera samples, with titers ranging between 1:80 and 1:5120. The nPCR based on the T. equi merozoite antigen gene (EMA-1) allowed the visualization of specie-specific amplified product in 108 (63.53%) horses. All six samples judged positive microscopically were also positive for nPCR. Statistical analysis indicated general disagreement (p < 0.0001) between IFAT and nPCR; IFAT and blood smear; and nPCR and blood smear on the detection of parasite carriers. The results of the present study indicate that T. equi is widely spread among horses in the Jaboticabal microregion, Northeast region of São Paulo State, Brazil.info:eu-repo/semantics/openAccessColégio Brasileiro de Parasitologia VeterináriaRevista Brasileira de Parasitologia Veterinária v.19 n.4 20102010-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612010000400007en10.1590/S1984-29612010000400007 |
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Baldani,Cristiane Divan Nakaghi,Andrea Cristina Higa Machado,Rosangela Zacarias |
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Baldani,Cristiane Divan Nakaghi,Andrea Cristina Higa Machado,Rosangela Zacarias Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil |
| author_facet |
Baldani,Cristiane Divan Nakaghi,Andrea Cristina Higa Machado,Rosangela Zacarias |
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Baldani,Cristiane Divan |
| title |
Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil |
| title_short |
Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil |
| title_full |
Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil |
| title_fullStr |
Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil |
| title_full_unstemmed |
Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, São Paulo State, Brazil |
| title_sort |
occurrence of theileria equi in horses raised in the jaboticabal microregion, são paulo state, brazil |
| description |
Blood and serum samples from 170 horses raised in the Jaboticabal microregion, São Paulo State, Brazil, were collected and tested by microscopic examination of blood smears, indirect fluorescent antibody test (IFAT) and nested polymerase chain reaction (nPCR) for Theileria equi infections. The association among the test results was verified by the McNemar test. During the examination of thin blood smears, parasites were detected in six (3.52%) horses. Anti-T. equi antibodies were detected in 100% sera samples, with titers ranging between 1:80 and 1:5120. The nPCR based on the T. equi merozoite antigen gene (EMA-1) allowed the visualization of specie-specific amplified product in 108 (63.53%) horses. All six samples judged positive microscopically were also positive for nPCR. Statistical analysis indicated general disagreement (p < 0.0001) between IFAT and nPCR; IFAT and blood smear; and nPCR and blood smear on the detection of parasite carriers. The results of the present study indicate that T. equi is widely spread among horses in the Jaboticabal microregion, Northeast region of São Paulo State, Brazil. |
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Colégio Brasileiro de Parasitologia Veterinária |
| publishDate |
2010 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612010000400007 |
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