Differences in Proteomic Profiles Between Caries Free and Caries Affected Children
Abstract Objective: To determine if there are differences in protein profiles in saliva depending if children of caries-free versus caries affected. Material and Methods: A cohort of 91 children with ages between 6 and 19 years, along clinical status of caries experience. Protein profiles in saliva were determined using electrophoresis and the calculation of the percentage of a specific band at a specific molecular weight in relationship to the total protein in that sample (% of total) using molecular weight standards. This quantification was repeated for each protein band across a range of molecular weights for each sample. Chi-square, Fisher’s exact, and Student t-tests were used to compare the distributions between caries-free and caries affected children (α=0.05). Results: Histatin was more likely to be non-detectable or reduced in caries-free children (OR=7.56; 95% CI 1.62-35.13) and these children had on average one less gel band detected by the assay we used. Conclusion: We have found differences in proteins between caries affected and caries-free children, suggesting that this line of investigation holds the promise of providing new tools for caries management.
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Associação de Apoio à Pesquisa em Saúde Bucal
2020
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oai:scielo:S1983-463220200001005012020-08-06Differences in Proteomic Profiles Between Caries Free and Caries Affected ChildrenVieira,Alexandre RezendeVieira,Nicholas ModestoLimesand,KirstenModesto,Adriana Dental Caries Proteome Proteomics Saliva Abstract Objective: To determine if there are differences in protein profiles in saliva depending if children of caries-free versus caries affected. Material and Methods: A cohort of 91 children with ages between 6 and 19 years, along clinical status of caries experience. Protein profiles in saliva were determined using electrophoresis and the calculation of the percentage of a specific band at a specific molecular weight in relationship to the total protein in that sample (% of total) using molecular weight standards. This quantification was repeated for each protein band across a range of molecular weights for each sample. Chi-square, Fisher’s exact, and Student t-tests were used to compare the distributions between caries-free and caries affected children (α=0.05). Results: Histatin was more likely to be non-detectable or reduced in caries-free children (OR=7.56; 95% CI 1.62-35.13) and these children had on average one less gel band detected by the assay we used. Conclusion: We have found differences in proteins between caries affected and caries-free children, suggesting that this line of investigation holds the promise of providing new tools for caries management.info:eu-repo/semantics/openAccessAssociação de Apoio à Pesquisa em Saúde BucalPesquisa Brasileira em Odontopediatria e Clínica Integrada v.20 20202020-01-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-46322020000100501en10.1590/pboci.2020.131 |
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Vieira,Alexandre Rezende Vieira,Nicholas Modesto Limesand,Kirsten Modesto,Adriana |
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Vieira,Alexandre Rezende Vieira,Nicholas Modesto Limesand,Kirsten Modesto,Adriana Differences in Proteomic Profiles Between Caries Free and Caries Affected Children |
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Vieira,Alexandre Rezende Vieira,Nicholas Modesto Limesand,Kirsten Modesto,Adriana |
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Vieira,Alexandre Rezende |
title |
Differences in Proteomic Profiles Between Caries Free and Caries Affected Children |
title_short |
Differences in Proteomic Profiles Between Caries Free and Caries Affected Children |
title_full |
Differences in Proteomic Profiles Between Caries Free and Caries Affected Children |
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Differences in Proteomic Profiles Between Caries Free and Caries Affected Children |
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Differences in Proteomic Profiles Between Caries Free and Caries Affected Children |
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differences in proteomic profiles between caries free and caries affected children |
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Abstract Objective: To determine if there are differences in protein profiles in saliva depending if children of caries-free versus caries affected. Material and Methods: A cohort of 91 children with ages between 6 and 19 years, along clinical status of caries experience. Protein profiles in saliva were determined using electrophoresis and the calculation of the percentage of a specific band at a specific molecular weight in relationship to the total protein in that sample (% of total) using molecular weight standards. This quantification was repeated for each protein band across a range of molecular weights for each sample. Chi-square, Fisher’s exact, and Student t-tests were used to compare the distributions between caries-free and caries affected children (α=0.05). Results: Histatin was more likely to be non-detectable or reduced in caries-free children (OR=7.56; 95% CI 1.62-35.13) and these children had on average one less gel band detected by the assay we used. Conclusion: We have found differences in proteins between caries affected and caries-free children, suggesting that this line of investigation holds the promise of providing new tools for caries management. |
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Associação de Apoio à Pesquisa em Saúde Bucal |
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2020 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-46322020000100501 |
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AT vieiraalexandrerezende differencesinproteomicprofilesbetweencariesfreeandcariesaffectedchildren AT vieiranicholasmodesto differencesinproteomicprofilesbetweencariesfreeandcariesaffectedchildren AT limesandkirsten differencesinproteomicprofilesbetweencariesfreeandcariesaffectedchildren AT modestoadriana differencesinproteomicprofilesbetweencariesfreeandcariesaffectedchildren |
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