Use of osmotic solutions for inhibition of sunflower seed germination in blotter test

The aim of this work was to investigate the feasibility of using a water restriction technique with osmotic solutes to control sunflower (Helianthus annuus L.) seed germination in blotter tests. The effects of water potential (-0.8 MPa - Megapascal, -1.0 MPa, -1.2 MPa and -1.4 MPa) of substrates osmotically modified with sodium chloride or mannitol were evaluated for seed germination, radicle length and mycoflora present in the samples. The effects of levels of osmotic potentials ranging from -0.35 MPa to -1.4 MPa were also investigated on mycelial growth of the fungi Alternaria alternata and Alternaria zinniae on PDA medium. All osmotic potentials were able to inhibit or to retard seed germination to the extent that seed examination in stereomicroscope became easier without affecting seed mycoflora associated with the seeds. Mycelial growth of A. alternata was stimulated by all osmotic solutes tested, while mycelial growth of A. zinniae was stimulated by mannitol and reduced by all NaCl osmotic potential levels used.

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Main Authors: Falleiro,Beatriz Alemonge de S., Almeida,Pollyne Borborema A. de, Coutinho,Wirton M., Suassuna,Nelson D., Kobayasti,Leimi
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Fitopatologia 2010
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762010000600002
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spelling oai:scielo:S1982-567620100006000022011-03-02Use of osmotic solutions for inhibition of sunflower seed germination in blotter testFalleiro,Beatriz Alemonge de S.Almeida,Pollyne Borborema A. deCoutinho,Wirton M.Suassuna,Nelson D.Kobayasti,Leimi Helianthus annuus sodium chloride mannitol The aim of this work was to investigate the feasibility of using a water restriction technique with osmotic solutes to control sunflower (Helianthus annuus L.) seed germination in blotter tests. The effects of water potential (-0.8 MPa - Megapascal, -1.0 MPa, -1.2 MPa and -1.4 MPa) of substrates osmotically modified with sodium chloride or mannitol were evaluated for seed germination, radicle length and mycoflora present in the samples. The effects of levels of osmotic potentials ranging from -0.35 MPa to -1.4 MPa were also investigated on mycelial growth of the fungi Alternaria alternata and Alternaria zinniae on PDA medium. All osmotic potentials were able to inhibit or to retard seed germination to the extent that seed examination in stereomicroscope became easier without affecting seed mycoflora associated with the seeds. Mycelial growth of A. alternata was stimulated by all osmotic solutes tested, while mycelial growth of A. zinniae was stimulated by mannitol and reduced by all NaCl osmotic potential levels used.info:eu-repo/semantics/openAccessSociedade Brasileira de FitopatologiaTropical Plant Pathology v.35 n.6 20102010-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762010000600002en10.1590/S1982-56762010000600002
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country Brasil
countrycode BR
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region America del Sur
libraryname SciELO
language English
format Digital
author Falleiro,Beatriz Alemonge de S.
Almeida,Pollyne Borborema A. de
Coutinho,Wirton M.
Suassuna,Nelson D.
Kobayasti,Leimi
spellingShingle Falleiro,Beatriz Alemonge de S.
Almeida,Pollyne Borborema A. de
Coutinho,Wirton M.
Suassuna,Nelson D.
Kobayasti,Leimi
Use of osmotic solutions for inhibition of sunflower seed germination in blotter test
author_facet Falleiro,Beatriz Alemonge de S.
Almeida,Pollyne Borborema A. de
Coutinho,Wirton M.
Suassuna,Nelson D.
Kobayasti,Leimi
author_sort Falleiro,Beatriz Alemonge de S.
title Use of osmotic solutions for inhibition of sunflower seed germination in blotter test
title_short Use of osmotic solutions for inhibition of sunflower seed germination in blotter test
title_full Use of osmotic solutions for inhibition of sunflower seed germination in blotter test
title_fullStr Use of osmotic solutions for inhibition of sunflower seed germination in blotter test
title_full_unstemmed Use of osmotic solutions for inhibition of sunflower seed germination in blotter test
title_sort use of osmotic solutions for inhibition of sunflower seed germination in blotter test
description The aim of this work was to investigate the feasibility of using a water restriction technique with osmotic solutes to control sunflower (Helianthus annuus L.) seed germination in blotter tests. The effects of water potential (-0.8 MPa - Megapascal, -1.0 MPa, -1.2 MPa and -1.4 MPa) of substrates osmotically modified with sodium chloride or mannitol were evaluated for seed germination, radicle length and mycoflora present in the samples. The effects of levels of osmotic potentials ranging from -0.35 MPa to -1.4 MPa were also investigated on mycelial growth of the fungi Alternaria alternata and Alternaria zinniae on PDA medium. All osmotic potentials were able to inhibit or to retard seed germination to the extent that seed examination in stereomicroscope became easier without affecting seed mycoflora associated with the seeds. Mycelial growth of A. alternata was stimulated by all osmotic solutes tested, while mycelial growth of A. zinniae was stimulated by mannitol and reduced by all NaCl osmotic potential levels used.
publisher Sociedade Brasileira de Fitopatologia
publishDate 2010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762010000600002
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