Detection of Listeria spp. in food handling areas of retail food stores in the state of Pernambuco, Brazil
Abstract The identification of Listeria spp. in food handling areas is of great concern to health surveillance agencies, and their control is often hampered by the ability of the bacteria to grow and maintain themselves even under adverse conditions. The present study aimed to isolate and identify Listeria spp. in the food handling areas of 10 retail food stores in the state of Pernambuco, Brazil. Eighty-six swab samples were collected from equipment, utensils and surfaces used for processing ready-to-eat meat products. The Dry and Wet Swabbing Methods (3M™ Quick Swabs) and 3M™ Petrifilm™ Plates were used to identify Listeria spp. Contamination by Listeria monocytogenes was confirmed by the Real-time Polymerase Chain Reaction (qPCR). The hygienic and sanitary conditions of the food handling areas of each store were also assessed. Listeria spp. was isolated in eight stores (80%). Of the 86 swab samples analyzed, 27 (31.2%) [confidence interval 21.81% to 42.30%] were positive for Listeria spp. and only one (3.7%) was confirmed as Listeria monocytogenes. The main contamination sites were the floor (50.0%), the plastic cutting board (42.9%) and the knife (40.0%). None of the hygienic and sanitary conditions assessed in the present study were associated with contamination by Listeria spp. (p = 0.700). It was concluded that Listeria spp. was widely distributed in the retail food stores studied, being a possible risk factor for public health.
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Instituto de Tecnologia de Alimentos - ITAL
2017
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oai:scielo:S1981-672320170001004422017-07-11Detection of Listeria spp. in food handling areas of retail food stores in the state of Pernambuco, BrazilSiqueira,Mariana Gomes Ferreira Machado deSilva,Jean Carlos Ramos daLúcio,Érica ChavesKim,Pomy de Cássia PeixotoLins,Leandro FragosoPinheiro Junior,José WiltonMota,Rinaldo AparecidoMoura,Andréa Paiva Botelho Lapenda de Good manufacturing practices Listeria monocytogenes Public health Supermarket Abstract The identification of Listeria spp. in food handling areas is of great concern to health surveillance agencies, and their control is often hampered by the ability of the bacteria to grow and maintain themselves even under adverse conditions. The present study aimed to isolate and identify Listeria spp. in the food handling areas of 10 retail food stores in the state of Pernambuco, Brazil. Eighty-six swab samples were collected from equipment, utensils and surfaces used for processing ready-to-eat meat products. The Dry and Wet Swabbing Methods (3M™ Quick Swabs) and 3M™ Petrifilm™ Plates were used to identify Listeria spp. Contamination by Listeria monocytogenes was confirmed by the Real-time Polymerase Chain Reaction (qPCR). The hygienic and sanitary conditions of the food handling areas of each store were also assessed. Listeria spp. was isolated in eight stores (80%). Of the 86 swab samples analyzed, 27 (31.2%) [confidence interval 21.81% to 42.30%] were positive for Listeria spp. and only one (3.7%) was confirmed as Listeria monocytogenes. The main contamination sites were the floor (50.0%), the plastic cutting board (42.9%) and the knife (40.0%). None of the hygienic and sanitary conditions assessed in the present study were associated with contamination by Listeria spp. (p = 0.700). It was concluded that Listeria spp. was widely distributed in the retail food stores studied, being a possible risk factor for public health.info:eu-repo/semantics/openAccessInstituto de Tecnologia de Alimentos - ITALBrazilian Journal of Food Technology v.20 20172017-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232017000100442en10.1590/1981-6723.13816 |
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Siqueira,Mariana Gomes Ferreira Machado de Silva,Jean Carlos Ramos da Lúcio,Érica Chaves Kim,Pomy de Cássia Peixoto Lins,Leandro Fragoso Pinheiro Junior,José Wilton Mota,Rinaldo Aparecido Moura,Andréa Paiva Botelho Lapenda de |
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Siqueira,Mariana Gomes Ferreira Machado de Silva,Jean Carlos Ramos da Lúcio,Érica Chaves Kim,Pomy de Cássia Peixoto Lins,Leandro Fragoso Pinheiro Junior,José Wilton Mota,Rinaldo Aparecido Moura,Andréa Paiva Botelho Lapenda de Detection of Listeria spp. in food handling areas of retail food stores in the state of Pernambuco, Brazil |
author_facet |
Siqueira,Mariana Gomes Ferreira Machado de Silva,Jean Carlos Ramos da Lúcio,Érica Chaves Kim,Pomy de Cássia Peixoto Lins,Leandro Fragoso Pinheiro Junior,José Wilton Mota,Rinaldo Aparecido Moura,Andréa Paiva Botelho Lapenda de |
author_sort |
Siqueira,Mariana Gomes Ferreira Machado de |
title |
Detection of Listeria spp. in food handling areas of retail food stores in the state of Pernambuco, Brazil |
title_short |
Detection of Listeria spp. in food handling areas of retail food stores in the state of Pernambuco, Brazil |
title_full |
Detection of Listeria spp. in food handling areas of retail food stores in the state of Pernambuco, Brazil |
title_fullStr |
Detection of Listeria spp. in food handling areas of retail food stores in the state of Pernambuco, Brazil |
title_full_unstemmed |
Detection of Listeria spp. in food handling areas of retail food stores in the state of Pernambuco, Brazil |
title_sort |
detection of listeria spp. in food handling areas of retail food stores in the state of pernambuco, brazil |
description |
Abstract The identification of Listeria spp. in food handling areas is of great concern to health surveillance agencies, and their control is often hampered by the ability of the bacteria to grow and maintain themselves even under adverse conditions. The present study aimed to isolate and identify Listeria spp. in the food handling areas of 10 retail food stores in the state of Pernambuco, Brazil. Eighty-six swab samples were collected from equipment, utensils and surfaces used for processing ready-to-eat meat products. The Dry and Wet Swabbing Methods (3M™ Quick Swabs) and 3M™ Petrifilm™ Plates were used to identify Listeria spp. Contamination by Listeria monocytogenes was confirmed by the Real-time Polymerase Chain Reaction (qPCR). The hygienic and sanitary conditions of the food handling areas of each store were also assessed. Listeria spp. was isolated in eight stores (80%). Of the 86 swab samples analyzed, 27 (31.2%) [confidence interval 21.81% to 42.30%] were positive for Listeria spp. and only one (3.7%) was confirmed as Listeria monocytogenes. The main contamination sites were the floor (50.0%), the plastic cutting board (42.9%) and the knife (40.0%). None of the hygienic and sanitary conditions assessed in the present study were associated with contamination by Listeria spp. (p = 0.700). It was concluded that Listeria spp. was widely distributed in the retail food stores studied, being a possible risk factor for public health. |
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Instituto de Tecnologia de Alimentos - ITAL |
publishDate |
2017 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232017000100442 |
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