Evaluation of zebu nellore cattle blood samples using the Cell-Dyn 3500 hematology analyzer
The Cell-dyn 3500 is a multiparameter flow cytometer, which may analyze samples from several species performing several simultaneous analyses. It is able to perform white blood cells, red blood cells and platelet counts, besides differential leukocyte counts, packed cell volume and hemoglobin determination. Cell-Dyn 3500 performs total leukocyte count both optically and by impedance. The equipment may choose one or other method, based on the reliability of the results. Erythrocyte and platelet counts are determined by impedance. Leukocyte differentiation is based on an optical principle, using separation in multiangular polarized light. The objective of this study was to compare the results of complete blood count of Zebu Nellore heifers from Cell-dyn 3500, with those obtained from a semi-automated cell counter (Celm CC 510) and the manual technique. Blood samples were collected from the jugular vein in 5 mL EDTA vacuum tubes from 58 Nellore heifers, at 24 months of age. Samples were processed in parallel in the three different techniques. Results were analyzed using paired t test, Pearson's correlation and the Bland-Altmann method. There was a strong correlation for all parameters analyzed by Cell-Dyn 3500, manual method and semi-automated cell counter, except for basophils and monocytes counts. These results confirm that this analyzer is reliable for blood samples analysis of zebu cattle.
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Universidade Federal de Goiás
2014
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oai:scielo:S1809-689120140004000122015-01-22Evaluation of zebu nellore cattle blood samples using the Cell-Dyn 3500 hematology analyzerBorges,Alexandre SecorunAmorim,Rogério MartinsTakahira,Regina KiomiCosta,Joselito NunesAlencar,Nayro XavierPeixoto,Ana Paula CardosoKohayagawa,AguemiKuchembuck,Marcio Rubens Graf CC-510 Cell-Dyn 3500 hematology Nellore The Cell-dyn 3500 is a multiparameter flow cytometer, which may analyze samples from several species performing several simultaneous analyses. It is able to perform white blood cells, red blood cells and platelet counts, besides differential leukocyte counts, packed cell volume and hemoglobin determination. Cell-Dyn 3500 performs total leukocyte count both optically and by impedance. The equipment may choose one or other method, based on the reliability of the results. Erythrocyte and platelet counts are determined by impedance. Leukocyte differentiation is based on an optical principle, using separation in multiangular polarized light. The objective of this study was to compare the results of complete blood count of Zebu Nellore heifers from Cell-dyn 3500, with those obtained from a semi-automated cell counter (Celm CC 510) and the manual technique. Blood samples were collected from the jugular vein in 5 mL EDTA vacuum tubes from 58 Nellore heifers, at 24 months of age. Samples were processed in parallel in the three different techniques. Results were analyzed using paired t test, Pearson's correlation and the Bland-Altmann method. There was a strong correlation for all parameters analyzed by Cell-Dyn 3500, manual method and semi-automated cell counter, except for basophils and monocytes counts. These results confirm that this analyzer is reliable for blood samples analysis of zebu cattle.info:eu-repo/semantics/openAccessUniversidade Federal de GoiásCiência Animal Brasileira v.15 n.4 20142014-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1809-68912014000400012en10.1590/1089-6891v15i41755 |
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Borges,Alexandre Secorun Amorim,Rogério Martins Takahira,Regina Kiomi Costa,Joselito Nunes Alencar,Nayro Xavier Peixoto,Ana Paula Cardoso Kohayagawa,Aguemi Kuchembuck,Marcio Rubens Graf |
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Borges,Alexandre Secorun Amorim,Rogério Martins Takahira,Regina Kiomi Costa,Joselito Nunes Alencar,Nayro Xavier Peixoto,Ana Paula Cardoso Kohayagawa,Aguemi Kuchembuck,Marcio Rubens Graf Evaluation of zebu nellore cattle blood samples using the Cell-Dyn 3500 hematology analyzer |
author_facet |
Borges,Alexandre Secorun Amorim,Rogério Martins Takahira,Regina Kiomi Costa,Joselito Nunes Alencar,Nayro Xavier Peixoto,Ana Paula Cardoso Kohayagawa,Aguemi Kuchembuck,Marcio Rubens Graf |
author_sort |
Borges,Alexandre Secorun |
title |
Evaluation of zebu nellore cattle blood samples using the Cell-Dyn 3500 hematology analyzer |
title_short |
Evaluation of zebu nellore cattle blood samples using the Cell-Dyn 3500 hematology analyzer |
title_full |
Evaluation of zebu nellore cattle blood samples using the Cell-Dyn 3500 hematology analyzer |
title_fullStr |
Evaluation of zebu nellore cattle blood samples using the Cell-Dyn 3500 hematology analyzer |
title_full_unstemmed |
Evaluation of zebu nellore cattle blood samples using the Cell-Dyn 3500 hematology analyzer |
title_sort |
evaluation of zebu nellore cattle blood samples using the cell-dyn 3500 hematology analyzer |
description |
The Cell-dyn 3500 is a multiparameter flow cytometer, which may analyze samples from several species performing several simultaneous analyses. It is able to perform white blood cells, red blood cells and platelet counts, besides differential leukocyte counts, packed cell volume and hemoglobin determination. Cell-Dyn 3500 performs total leukocyte count both optically and by impedance. The equipment may choose one or other method, based on the reliability of the results. Erythrocyte and platelet counts are determined by impedance. Leukocyte differentiation is based on an optical principle, using separation in multiangular polarized light. The objective of this study was to compare the results of complete blood count of Zebu Nellore heifers from Cell-dyn 3500, with those obtained from a semi-automated cell counter (Celm CC 510) and the manual technique. Blood samples were collected from the jugular vein in 5 mL EDTA vacuum tubes from 58 Nellore heifers, at 24 months of age. Samples were processed in parallel in the three different techniques. Results were analyzed using paired t test, Pearson's correlation and the Bland-Altmann method. There was a strong correlation for all parameters analyzed by Cell-Dyn 3500, manual method and semi-automated cell counter, except for basophils and monocytes counts. These results confirm that this analyzer is reliable for blood samples analysis of zebu cattle. |
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Universidade Federal de Goiás |
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2014 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1809-68912014000400012 |
work_keys_str_mv |
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