Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians

Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians

ABSTRACT INTRODUCTION: Oral squamous cell carcinoma (OSCC) is a serious public health problem, due to its high mortality rate and worldwide rising incidence. OSCC susceptibility is mediated by interactions between genetic and environmental factors. Studies suggest that genetic variants encoding enzymes involved in folate metabolism may modulate OSCC risk by altering DNA synthesis/repair and methylation process. OBJECTIVE: The goals of this study were to evaluate the association of three genotypic polymorphism (MTHFR C677T, MTHFR A1298C and CBS 844ins68) and oral cancer risk in southeastern Brazilians and evaluate the interactions between polymorphisms and clinical histopathological parameters. METHODS: This case-control study included 101 cases and 102 controls in the state of Espírito Santo, Brazil. MTHFR genotyping was done by PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism) and CBS genotyping by PCR (polymerase chain reaction) analysis. RESULTS: MTHFR C677T polymorphism was associated with lymph node involvement. Genotype CT + TT acted as a protective factor. MTHFR A1298C AC + CC genotype was associated with tumor differentiation, and possibly with a better prognosis. In risk analysis, no correlation was observed between genotypes and OSCC. CONCLUSION: We concluded that MTHFR C677T, MTHFR A1298C and CBS 844ins68 polymorphisms were not associated with OSCC risk in southeastern Brazilians; however, we suggest a prognosis effect associated with MTHFR C677T and A1298C polymorphisms in OSCC.

Saved in:
Bibliographic Details
Main Authors: Barbosa,Andressa, Santos,Marcelo dos, Podestá,José Roberto Vasconcelos de, Gouvêa,Sônia Alves, Zeidler,Sandra Ventorin Von, Louro,Iúri Drumond, Cordeiro-Silva,Melissa de Freitas
Format: Digital revista
Language:English
Published: Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. 2016
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-86942016000500558
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S1808-86942016000500558
record_format ojs
spelling oai:scielo:S1808-869420160005005582016-11-09Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern BraziliansBarbosa,AndressaSantos,Marcelo dosPodestá,José Roberto Vasconcelos deGouvêa,Sônia AlvesZeidler,Sandra Ventorin VonLouro,Iúri DrumondCordeiro-Silva,Melissa de Freitas Oral squamous cell carcinoma Methylenetetrahydrofolate reductase Cystathionine beta-synthase Genetic polymorphism ABSTRACT INTRODUCTION: Oral squamous cell carcinoma (OSCC) is a serious public health problem, due to its high mortality rate and worldwide rising incidence. OSCC susceptibility is mediated by interactions between genetic and environmental factors. Studies suggest that genetic variants encoding enzymes involved in folate metabolism may modulate OSCC risk by altering DNA synthesis/repair and methylation process. OBJECTIVE: The goals of this study were to evaluate the association of three genotypic polymorphism (MTHFR C677T, MTHFR A1298C and CBS 844ins68) and oral cancer risk in southeastern Brazilians and evaluate the interactions between polymorphisms and clinical histopathological parameters. METHODS: This case-control study included 101 cases and 102 controls in the state of Espírito Santo, Brazil. MTHFR genotyping was done by PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism) and CBS genotyping by PCR (polymerase chain reaction) analysis. RESULTS: MTHFR C677T polymorphism was associated with lymph node involvement. Genotype CT + TT acted as a protective factor. MTHFR A1298C AC + CC genotype was associated with tumor differentiation, and possibly with a better prognosis. In risk analysis, no correlation was observed between genotypes and OSCC. CONCLUSION: We concluded that MTHFR C677T, MTHFR A1298C and CBS 844ins68 polymorphisms were not associated with OSCC risk in southeastern Brazilians; however, we suggest a prognosis effect associated with MTHFR C677T and A1298C polymorphisms in OSCC.info:eu-repo/semantics/openAccessAssociação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial.Brazilian Journal of Otorhinolaryngology v.82 n.5 20162016-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-86942016000500558en10.1016/j.bjorl.2015.10.012
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Barbosa,Andressa
Santos,Marcelo dos
Podestá,José Roberto Vasconcelos de
Gouvêa,Sônia Alves
Zeidler,Sandra Ventorin Von
Louro,Iúri Drumond
Cordeiro-Silva,Melissa de Freitas
spellingShingle Barbosa,Andressa
Santos,Marcelo dos
Podestá,José Roberto Vasconcelos de
Gouvêa,Sônia Alves
Zeidler,Sandra Ventorin Von
Louro,Iúri Drumond
Cordeiro-Silva,Melissa de Freitas
Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians
author_facet Barbosa,Andressa
Santos,Marcelo dos
Podestá,José Roberto Vasconcelos de
Gouvêa,Sônia Alves
Zeidler,Sandra Ventorin Von
Louro,Iúri Drumond
Cordeiro-Silva,Melissa de Freitas
author_sort Barbosa,Andressa
title Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians
title_short Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians
title_full Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians
title_fullStr Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians
title_full_unstemmed Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians
title_sort polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern brazilians
description ABSTRACT INTRODUCTION: Oral squamous cell carcinoma (OSCC) is a serious public health problem, due to its high mortality rate and worldwide rising incidence. OSCC susceptibility is mediated by interactions between genetic and environmental factors. Studies suggest that genetic variants encoding enzymes involved in folate metabolism may modulate OSCC risk by altering DNA synthesis/repair and methylation process. OBJECTIVE: The goals of this study were to evaluate the association of three genotypic polymorphism (MTHFR C677T, MTHFR A1298C and CBS 844ins68) and oral cancer risk in southeastern Brazilians and evaluate the interactions between polymorphisms and clinical histopathological parameters. METHODS: This case-control study included 101 cases and 102 controls in the state of Espírito Santo, Brazil. MTHFR genotyping was done by PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism) and CBS genotyping by PCR (polymerase chain reaction) analysis. RESULTS: MTHFR C677T polymorphism was associated with lymph node involvement. Genotype CT + TT acted as a protective factor. MTHFR A1298C AC + CC genotype was associated with tumor differentiation, and possibly with a better prognosis. In risk analysis, no correlation was observed between genotypes and OSCC. CONCLUSION: We concluded that MTHFR C677T, MTHFR A1298C and CBS 844ins68 polymorphisms were not associated with OSCC risk in southeastern Brazilians; however, we suggest a prognosis effect associated with MTHFR C677T and A1298C polymorphisms in OSCC.
publisher Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial.
publishDate 2016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-86942016000500558
work_keys_str_mv AT barbosaandressa polymorphismsinmethylenetetrahydrofolatereductaseandcystathioninebetasynthaseinoralcanceracasecontrolstudyinsoutheasternbrazilians
AT santosmarcelodos polymorphismsinmethylenetetrahydrofolatereductaseandcystathioninebetasynthaseinoralcanceracasecontrolstudyinsoutheasternbrazilians
AT podestajoserobertovasconcelosde polymorphismsinmethylenetetrahydrofolatereductaseandcystathioninebetasynthaseinoralcanceracasecontrolstudyinsoutheasternbrazilians
AT gouveasoniaalves polymorphismsinmethylenetetrahydrofolatereductaseandcystathioninebetasynthaseinoralcanceracasecontrolstudyinsoutheasternbrazilians
AT zeidlersandraventorinvon polymorphismsinmethylenetetrahydrofolatereductaseandcystathioninebetasynthaseinoralcanceracasecontrolstudyinsoutheasternbrazilians
AT louroiuridrumond polymorphismsinmethylenetetrahydrofolatereductaseandcystathioninebetasynthaseinoralcanceracasecontrolstudyinsoutheasternbrazilians
AT cordeirosilvamelissadefreitas polymorphismsinmethylenetetrahydrofolatereductaseandcystathioninebetasynthaseinoralcanceracasecontrolstudyinsoutheasternbrazilians
_version_ 1756433273558401024

Similar Items