Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi

Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi

ABSTRACT: Extracellular enzymes are involved in the fungal pathogenesis in plants. Currently, culture media, data analyses, and data report related to extracellular enzymes produced in vitro conditions are different and therefore, lack standardization. This work aimed to compare the culture media cited on the literature (normal) with the potato-dextrose-agar (PDA) medium combined with a specific compound to produce extracellular enzymes through three soilborne phytopathogenic fungi (F. solani f. sp. passiflorae, S. rolfsii, and R. solani AG-4 HGI), as well as to analyze and report enzyme data based on five different criteria. The assay was randomized, with three factors (culture media, isolates, and enzymes) and six repetitions. The studied enzymes were amylase (AM), carboxymethylcellulase (CMCase), lipase (LP), laccase (LC), catalase (CT), and gelatinase (GT). The normal media detected more enzymes and was more precise compared to the PDA medium plus specific compound. The criteria that calculated the area of the circular crown of AM, CMCase, LP, and LC and measured the intensity (0 = absence, up to 4 = intense) of CT and GT adopting note scale were the best to evaluate and report the results of the enzymes. We suggest the normal media culture to study enzyme production, as well as the criteria mentioned to assess and report the data related to enzyme activities.

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Main Authors: Ferreira,Ana Beatriz Monteiro, Fischer,Ivan Herman, Leite,Luís Garrigós, Padovani,Carlos Roberto, Bueno,César Júnior
Format: Digital revista
Language:English
Published: Instituto Biológico 2019
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100209
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spelling oai:scielo:S1808-165720190001002092019-07-24Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungiFerreira,Ana Beatriz MonteiroFischer,Ivan HermanLeite,Luís GarrigósPadovani,Carlos RobertoBueno,César Júnior enzymes soilborne phytopathogenic fungi methodology ABSTRACT: Extracellular enzymes are involved in the fungal pathogenesis in plants. Currently, culture media, data analyses, and data report related to extracellular enzymes produced in vitro conditions are different and therefore, lack standardization. This work aimed to compare the culture media cited on the literature (normal) with the potato-dextrose-agar (PDA) medium combined with a specific compound to produce extracellular enzymes through three soilborne phytopathogenic fungi (F. solani f. sp. passiflorae, S. rolfsii, and R. solani AG-4 HGI), as well as to analyze and report enzyme data based on five different criteria. The assay was randomized, with three factors (culture media, isolates, and enzymes) and six repetitions. The studied enzymes were amylase (AM), carboxymethylcellulase (CMCase), lipase (LP), laccase (LC), catalase (CT), and gelatinase (GT). The normal media detected more enzymes and was more precise compared to the PDA medium plus specific compound. The criteria that calculated the area of the circular crown of AM, CMCase, LP, and LC and measured the intensity (0 = absence, up to 4 = intense) of CT and GT adopting note scale were the best to evaluate and report the results of the enzymes. We suggest the normal media culture to study enzyme production, as well as the criteria mentioned to assess and report the data related to enzyme activities.info:eu-repo/semantics/openAccessInstituto BiológicoArquivos do Instituto Biológico v.86 20192019-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100209en10.1590/1808-1657000592017
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Ferreira,Ana Beatriz Monteiro
Fischer,Ivan Herman
Leite,Luís Garrigós
Padovani,Carlos Roberto
Bueno,César Júnior
spellingShingle Ferreira,Ana Beatriz Monteiro
Fischer,Ivan Herman
Leite,Luís Garrigós
Padovani,Carlos Roberto
Bueno,César Júnior
Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
author_facet Ferreira,Ana Beatriz Monteiro
Fischer,Ivan Herman
Leite,Luís Garrigós
Padovani,Carlos Roberto
Bueno,César Júnior
author_sort Ferreira,Ana Beatriz Monteiro
title Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
title_short Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
title_full Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
title_fullStr Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
title_full_unstemmed Culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
title_sort culture media to detect and criteria to evaluate and report the activity of extracellular enzymes produced by phytopathogenic fungi
description ABSTRACT: Extracellular enzymes are involved in the fungal pathogenesis in plants. Currently, culture media, data analyses, and data report related to extracellular enzymes produced in vitro conditions are different and therefore, lack standardization. This work aimed to compare the culture media cited on the literature (normal) with the potato-dextrose-agar (PDA) medium combined with a specific compound to produce extracellular enzymes through three soilborne phytopathogenic fungi (F. solani f. sp. passiflorae, S. rolfsii, and R. solani AG-4 HGI), as well as to analyze and report enzyme data based on five different criteria. The assay was randomized, with three factors (culture media, isolates, and enzymes) and six repetitions. The studied enzymes were amylase (AM), carboxymethylcellulase (CMCase), lipase (LP), laccase (LC), catalase (CT), and gelatinase (GT). The normal media detected more enzymes and was more precise compared to the PDA medium plus specific compound. The criteria that calculated the area of the circular crown of AM, CMCase, LP, and LC and measured the intensity (0 = absence, up to 4 = intense) of CT and GT adopting note scale were the best to evaluate and report the results of the enzymes. We suggest the normal media culture to study enzyme production, as well as the criteria mentioned to assess and report the data related to enzyme activities.
publisher Instituto Biológico
publishDate 2019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100209
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