Moderate levels of physical fitness maintain telomere length in non-senescent T CD8+ cells of aged men
OBJECTIVES: Immunosenescence is an age-associated change characterized by a decreased immune response. Although physical activity has been described as fundamental for maintaining the quality of life, few studies have evaluated the effects of different levels of exercise on telomere length in aged populations. The present study aimed to analyze the effects of different levels of physical activity, classified by the Maximal oxygen consumption (VO2 max) values, on the telomere length of memory Cluster of differentiation (CD) CD4+(CD45ROneg and CD45RO+), effector CD8+CD28neg, and CD8+CD28+ T cells in aged individuals. METHODS: Fifty-three healthy elderly men (aged 65-85 years) were included in this study. Their fitness level was classified according to the American College of Sports Medicine (ACSM) for VO2 max (mL/kg/min). Blood samples were obtained from all participants to analyze the percentage of CD3, CD4, CD8, CD28+, naïve, and subpopulations of memory T cells by using flow cytometry. Furthermore, using the Flow-FISH methodology, the CD4+CD45RO+, CD4+CD45ROneg, CD8+CD28+, and CD8+CD28negT cell telomere lengths were measured. RESULTS: There was a greater proportion of effector memory T CD4+ cells and longer telomeres in CD8+CD28+ T cells in the moderate physical fitness group than in the other groups. There was a higher proportion of terminally differentiated memory effector T cells in the low physical fitness group. CONCLUSION: A moderate physical activity may positively influence the telomere shortening of CD28+CD8+T cells. However, additional studies are necessary to evaluate the importance of this finding with regard to immune function responses in older men.
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Faculdade de Medicina / USP
2020
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oai:scielo:S1807-593220200001003032020-11-03Moderate levels of physical fitness maintain telomere length in non-senescent T CD8+ cells of aged menBastos,Marta FerreiraMatias,Manuella de Sousa ToledoAlonso,Angélica CastilhoSilva,Léia Cristina Rodriguesde Araújo,Adriana LadeiraSilva,Paulo RobertoBenard,GilBocalini,Danilo SalesSteven Baker,JulienLeme,Luiz Eugênio Garcez Immunosenescence Aging Exercise Telomere T cells CD28 Physical fitness OBJECTIVES: Immunosenescence is an age-associated change characterized by a decreased immune response. Although physical activity has been described as fundamental for maintaining the quality of life, few studies have evaluated the effects of different levels of exercise on telomere length in aged populations. The present study aimed to analyze the effects of different levels of physical activity, classified by the Maximal oxygen consumption (VO2 max) values, on the telomere length of memory Cluster of differentiation (CD) CD4+(CD45ROneg and CD45RO+), effector CD8+CD28neg, and CD8+CD28+ T cells in aged individuals. METHODS: Fifty-three healthy elderly men (aged 65-85 years) were included in this study. Their fitness level was classified according to the American College of Sports Medicine (ACSM) for VO2 max (mL/kg/min). Blood samples were obtained from all participants to analyze the percentage of CD3, CD4, CD8, CD28+, naïve, and subpopulations of memory T cells by using flow cytometry. Furthermore, using the Flow-FISH methodology, the CD4+CD45RO+, CD4+CD45ROneg, CD8+CD28+, and CD8+CD28negT cell telomere lengths were measured. RESULTS: There was a greater proportion of effector memory T CD4+ cells and longer telomeres in CD8+CD28+ T cells in the moderate physical fitness group than in the other groups. There was a higher proportion of terminally differentiated memory effector T cells in the low physical fitness group. CONCLUSION: A moderate physical activity may positively influence the telomere shortening of CD28+CD8+T cells. However, additional studies are necessary to evaluate the importance of this finding with regard to immune function responses in older men.info:eu-repo/semantics/openAccessFaculdade de Medicina / USPClinics v.75 20202020-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322020000100303en10.6061/clinics/2020/e1628 |
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Bastos,Marta Ferreira Matias,Manuella de Sousa Toledo Alonso,Angélica Castilho Silva,Léia Cristina Rodrigues de Araújo,Adriana Ladeira Silva,Paulo Roberto Benard,Gil Bocalini,Danilo Sales Steven Baker,Julien Leme,Luiz Eugênio Garcez |
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Bastos,Marta Ferreira Matias,Manuella de Sousa Toledo Alonso,Angélica Castilho Silva,Léia Cristina Rodrigues de Araújo,Adriana Ladeira Silva,Paulo Roberto Benard,Gil Bocalini,Danilo Sales Steven Baker,Julien Leme,Luiz Eugênio Garcez Moderate levels of physical fitness maintain telomere length in non-senescent T CD8+ cells of aged men |
author_facet |
Bastos,Marta Ferreira Matias,Manuella de Sousa Toledo Alonso,Angélica Castilho Silva,Léia Cristina Rodrigues de Araújo,Adriana Ladeira Silva,Paulo Roberto Benard,Gil Bocalini,Danilo Sales Steven Baker,Julien Leme,Luiz Eugênio Garcez |
author_sort |
Bastos,Marta Ferreira |
title |
Moderate levels of physical fitness maintain telomere length in non-senescent T CD8+ cells of aged men |
title_short |
Moderate levels of physical fitness maintain telomere length in non-senescent T CD8+ cells of aged men |
title_full |
Moderate levels of physical fitness maintain telomere length in non-senescent T CD8+ cells of aged men |
title_fullStr |
Moderate levels of physical fitness maintain telomere length in non-senescent T CD8+ cells of aged men |
title_full_unstemmed |
Moderate levels of physical fitness maintain telomere length in non-senescent T CD8+ cells of aged men |
title_sort |
moderate levels of physical fitness maintain telomere length in non-senescent t cd8+ cells of aged men |
description |
OBJECTIVES: Immunosenescence is an age-associated change characterized by a decreased immune response. Although physical activity has been described as fundamental for maintaining the quality of life, few studies have evaluated the effects of different levels of exercise on telomere length in aged populations. The present study aimed to analyze the effects of different levels of physical activity, classified by the Maximal oxygen consumption (VO2 max) values, on the telomere length of memory Cluster of differentiation (CD) CD4+(CD45ROneg and CD45RO+), effector CD8+CD28neg, and CD8+CD28+ T cells in aged individuals. METHODS: Fifty-three healthy elderly men (aged 65-85 years) were included in this study. Their fitness level was classified according to the American College of Sports Medicine (ACSM) for VO2 max (mL/kg/min). Blood samples were obtained from all participants to analyze the percentage of CD3, CD4, CD8, CD28+, naïve, and subpopulations of memory T cells by using flow cytometry. Furthermore, using the Flow-FISH methodology, the CD4+CD45RO+, CD4+CD45ROneg, CD8+CD28+, and CD8+CD28negT cell telomere lengths were measured. RESULTS: There was a greater proportion of effector memory T CD4+ cells and longer telomeres in CD8+CD28+ T cells in the moderate physical fitness group than in the other groups. There was a higher proportion of terminally differentiated memory effector T cells in the low physical fitness group. CONCLUSION: A moderate physical activity may positively influence the telomere shortening of CD28+CD8+T cells. However, additional studies are necessary to evaluate the importance of this finding with regard to immune function responses in older men. |
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Faculdade de Medicina / USP |
publishDate |
2020 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322020000100303 |
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