Immunohistochemical assessment of mucosal cytokine profile in acetic acid experimental colitis
Experimental colitis induced by acetic acid has been used extensively as a model for intestinal inflammatory disease. Colonic tissue lesions of intestinal inflammatory disease patients seem to be related to the increased local production of pro-inflammatory cytokines (IL-1, IL-6, TNF-alpha, and IFN-gamma). PURPOSE: To assess the cytokine expression pattern identified through immunohistochemistry in colonic mucosa after experimental colitis induced by acetic acid and establish the relationship between this pattern and the presence of macroscopic lesions. MATERIALS AND METHODS: Adult male Wistar rats (n = 39) were divided at random into 4 groups: NC45 and NC24 (control without colitis; sacrificed at 45 minutes and 24 hours, respectively); and WC45 and WC24 (with experimental colitis induced by acetic acid; sacrificed at 45 minutes and 24 hours, respectively). Macroscopic and microscopic alterations in colonic tissue were evaluated, and cytokine expression was assessed through immunohistochemistry. RESULTS: After 24 hours, IL-1 expression was greater in the groups with colitis when compared to the groups without colitis. IL-4 expression was higher in the WC45 group. There was an increase in both INF-gamma and IL-6 related to the presence of necrosis of the colonic mucosa in the groups with colitis for both periods evaluated. CONCLUSION: The immunohistochemical technique was efficient for the analysis of various cytokine expressions in the colonic tissue. There was an increase in the IL-1 pro-inflammatory cytokines as well as in IL-6 and IFN-gamma associated with the presence of colonic necrosis. Experimental colitis induced by acetic acid is a useful model for the development of studies assessing the role of cytokines in the inflammation of mucosa as well as anti-cytokine therapies.
Main Authors: | , , , , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Faculdade de Medicina / USP
2005
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322005000400004 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S1807-59322005000400004 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S1807-593220050004000042005-08-29Immunohistochemical assessment of mucosal cytokine profile in acetic acid experimental colitisBertevello,Pedro L.Logullo,Ângela FláviaNonogaki,SueliCampos,Fabio M.Chiferi,ValcirAlves,Claudia C.Torrinhas,Raquel S.Gama-Rodrigues,Joaquim JoséWaitzberg,Dan L. Cytokines Rats Inflammatory colitis Immunohistochemistry Necrosis Experimental colitis induced by acetic acid has been used extensively as a model for intestinal inflammatory disease. Colonic tissue lesions of intestinal inflammatory disease patients seem to be related to the increased local production of pro-inflammatory cytokines (IL-1, IL-6, TNF-alpha, and IFN-gamma). PURPOSE: To assess the cytokine expression pattern identified through immunohistochemistry in colonic mucosa after experimental colitis induced by acetic acid and establish the relationship between this pattern and the presence of macroscopic lesions. MATERIALS AND METHODS: Adult male Wistar rats (n = 39) were divided at random into 4 groups: NC45 and NC24 (control without colitis; sacrificed at 45 minutes and 24 hours, respectively); and WC45 and WC24 (with experimental colitis induced by acetic acid; sacrificed at 45 minutes and 24 hours, respectively). Macroscopic and microscopic alterations in colonic tissue were evaluated, and cytokine expression was assessed through immunohistochemistry. RESULTS: After 24 hours, IL-1 expression was greater in the groups with colitis when compared to the groups without colitis. IL-4 expression was higher in the WC45 group. There was an increase in both INF-gamma and IL-6 related to the presence of necrosis of the colonic mucosa in the groups with colitis for both periods evaluated. CONCLUSION: The immunohistochemical technique was efficient for the analysis of various cytokine expressions in the colonic tissue. There was an increase in the IL-1 pro-inflammatory cytokines as well as in IL-6 and IFN-gamma associated with the presence of colonic necrosis. Experimental colitis induced by acetic acid is a useful model for the development of studies assessing the role of cytokines in the inflammation of mucosa as well as anti-cytokine therapies.info:eu-repo/semantics/openAccessFaculdade de Medicina / USPClinics v.60 n.4 20052005-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322005000400004en10.1590/S1807-59322005000400004 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Bertevello,Pedro L. Logullo,Ângela Flávia Nonogaki,Sueli Campos,Fabio M. Chiferi,Valcir Alves,Claudia C. Torrinhas,Raquel S. Gama-Rodrigues,Joaquim José Waitzberg,Dan L. |
spellingShingle |
Bertevello,Pedro L. Logullo,Ângela Flávia Nonogaki,Sueli Campos,Fabio M. Chiferi,Valcir Alves,Claudia C. Torrinhas,Raquel S. Gama-Rodrigues,Joaquim José Waitzberg,Dan L. Immunohistochemical assessment of mucosal cytokine profile in acetic acid experimental colitis |
author_facet |
Bertevello,Pedro L. Logullo,Ângela Flávia Nonogaki,Sueli Campos,Fabio M. Chiferi,Valcir Alves,Claudia C. Torrinhas,Raquel S. Gama-Rodrigues,Joaquim José Waitzberg,Dan L. |
author_sort |
Bertevello,Pedro L. |
title |
Immunohistochemical assessment of mucosal cytokine profile in acetic acid experimental colitis |
title_short |
Immunohistochemical assessment of mucosal cytokine profile in acetic acid experimental colitis |
title_full |
Immunohistochemical assessment of mucosal cytokine profile in acetic acid experimental colitis |
title_fullStr |
Immunohistochemical assessment of mucosal cytokine profile in acetic acid experimental colitis |
title_full_unstemmed |
Immunohistochemical assessment of mucosal cytokine profile in acetic acid experimental colitis |
title_sort |
immunohistochemical assessment of mucosal cytokine profile in acetic acid experimental colitis |
description |
Experimental colitis induced by acetic acid has been used extensively as a model for intestinal inflammatory disease. Colonic tissue lesions of intestinal inflammatory disease patients seem to be related to the increased local production of pro-inflammatory cytokines (IL-1, IL-6, TNF-alpha, and IFN-gamma). PURPOSE: To assess the cytokine expression pattern identified through immunohistochemistry in colonic mucosa after experimental colitis induced by acetic acid and establish the relationship between this pattern and the presence of macroscopic lesions. MATERIALS AND METHODS: Adult male Wistar rats (n = 39) were divided at random into 4 groups: NC45 and NC24 (control without colitis; sacrificed at 45 minutes and 24 hours, respectively); and WC45 and WC24 (with experimental colitis induced by acetic acid; sacrificed at 45 minutes and 24 hours, respectively). Macroscopic and microscopic alterations in colonic tissue were evaluated, and cytokine expression was assessed through immunohistochemistry. RESULTS: After 24 hours, IL-1 expression was greater in the groups with colitis when compared to the groups without colitis. IL-4 expression was higher in the WC45 group. There was an increase in both INF-gamma and IL-6 related to the presence of necrosis of the colonic mucosa in the groups with colitis for both periods evaluated. CONCLUSION: The immunohistochemical technique was efficient for the analysis of various cytokine expressions in the colonic tissue. There was an increase in the IL-1 pro-inflammatory cytokines as well as in IL-6 and IFN-gamma associated with the presence of colonic necrosis. Experimental colitis induced by acetic acid is a useful model for the development of studies assessing the role of cytokines in the inflammation of mucosa as well as anti-cytokine therapies. |
publisher |
Faculdade de Medicina / USP |
publishDate |
2005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322005000400004 |
work_keys_str_mv |
AT bertevellopedrol immunohistochemicalassessmentofmucosalcytokineprofileinaceticacidexperimentalcolitis AT logulloangelaflavia immunohistochemicalassessmentofmucosalcytokineprofileinaceticacidexperimentalcolitis AT nonogakisueli immunohistochemicalassessmentofmucosalcytokineprofileinaceticacidexperimentalcolitis AT camposfabiom immunohistochemicalassessmentofmucosalcytokineprofileinaceticacidexperimentalcolitis AT chiferivalcir immunohistochemicalassessmentofmucosalcytokineprofileinaceticacidexperimentalcolitis AT alvesclaudiac immunohistochemicalassessmentofmucosalcytokineprofileinaceticacidexperimentalcolitis AT torrinhasraquels immunohistochemicalassessmentofmucosalcytokineprofileinaceticacidexperimentalcolitis AT gamarodriguesjoaquimjose immunohistochemicalassessmentofmucosalcytokineprofileinaceticacidexperimentalcolitis AT waitzbergdanl immunohistochemicalassessmentofmucosalcytokineprofileinaceticacidexperimentalcolitis |
_version_ |
1756431974539460608 |