DNA damage-related proteins in smokers and non-smokers with oral cancer
Abstract: Tobacco smoking involves a high risk of human malignancies, including oral cancer, because it contains multiple carcinogens that cause genetic instability. Thus, a worse prognosis would be expected for cancer patients who are smokers. The aim of this study was to assess the DNA damage response through the expression of checkpoint kinase 2 (CHK2), H2A histone family member X (H2AX), and P53 among smokers and non-smokers with oral squamous cell carcinoma (OSCC). Associations between immunoexpression of proteins and clinicopathological data and histopathological grading were also analyzed. A total of 35 individuals (18 non-smokers and 17 smokers) with OSCC of the tongue and/or floor of the mouth were included. Immunohistochemistry for H2AX was conducted for the identification of double-strand breaks, CHK2, and P53 to evaluate the expression of this protein in cell cycle regulation. The sample consisted of 22 males and 13 females, with a mean age of 63.9±11.8 years. OSCC of non-smokers were well-differentiated tumors in 50% of the cases, and those of smokers were equally distributed into moderately differentiated and poorly differentiated tumors (35.3% each). Overall, 31 (88.6%) cases were CHK2-positive, 27 (77.1%) were H2AX-positive, and 23 (65.7%) were P53-positive, with no difference between smokers and non-smokers (p > 0.05). No association was found between proteins and clinicopathologic data (p > 0.05). Similarities in CHK2, H2AX, and P53 immunohistochemical staining patterns were observed between smokers and non-smokers, and immunoexpression was not associated with clinicopathological parameters. However, the findings indicated consistent expression of these proteins in OSCC.
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Sociedade Brasileira de Pesquisa Odontológica - SBPqO
2022
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oai:scielo:S1806-832420220001002212022-03-17DNA damage-related proteins in smokers and non-smokers with oral cancerSchuch,Lauren Frenzelde Arruda,José Alcides AlmeidaViana,Karolina Skarlet SilvaCaldeira,Patrícia CarlosAbreu,Mauro Henrique Nogueira GuimarãesBernardes,Vanessa FátimaAguiar,Maria Cássia Ferreira de DNA Damage Checkpoint Kinase 2 Tumor Suppressor Protein p53 Mouth Neoplasms Abstract: Tobacco smoking involves a high risk of human malignancies, including oral cancer, because it contains multiple carcinogens that cause genetic instability. Thus, a worse prognosis would be expected for cancer patients who are smokers. The aim of this study was to assess the DNA damage response through the expression of checkpoint kinase 2 (CHK2), H2A histone family member X (H2AX), and P53 among smokers and non-smokers with oral squamous cell carcinoma (OSCC). Associations between immunoexpression of proteins and clinicopathological data and histopathological grading were also analyzed. A total of 35 individuals (18 non-smokers and 17 smokers) with OSCC of the tongue and/or floor of the mouth were included. Immunohistochemistry for H2AX was conducted for the identification of double-strand breaks, CHK2, and P53 to evaluate the expression of this protein in cell cycle regulation. The sample consisted of 22 males and 13 females, with a mean age of 63.9±11.8 years. OSCC of non-smokers were well-differentiated tumors in 50% of the cases, and those of smokers were equally distributed into moderately differentiated and poorly differentiated tumors (35.3% each). Overall, 31 (88.6%) cases were CHK2-positive, 27 (77.1%) were H2AX-positive, and 23 (65.7%) were P53-positive, with no difference between smokers and non-smokers (p > 0.05). No association was found between proteins and clinicopathologic data (p > 0.05). Similarities in CHK2, H2AX, and P53 immunohistochemical staining patterns were observed between smokers and non-smokers, and immunoexpression was not associated with clinicopathological parameters. However, the findings indicated consistent expression of these proteins in OSCC.info:eu-repo/semantics/openAccessSociedade Brasileira de Pesquisa Odontológica - SBPqOBrazilian Oral Research v.36 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100221en10.1590/1807-3107bor-2022.vol36.0027 |
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Schuch,Lauren Frenzel de Arruda,José Alcides Almeida Viana,Karolina Skarlet Silva Caldeira,Patrícia Carlos Abreu,Mauro Henrique Nogueira Guimarães Bernardes,Vanessa Fátima Aguiar,Maria Cássia Ferreira de |
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Schuch,Lauren Frenzel de Arruda,José Alcides Almeida Viana,Karolina Skarlet Silva Caldeira,Patrícia Carlos Abreu,Mauro Henrique Nogueira Guimarães Bernardes,Vanessa Fátima Aguiar,Maria Cássia Ferreira de DNA damage-related proteins in smokers and non-smokers with oral cancer |
author_facet |
Schuch,Lauren Frenzel de Arruda,José Alcides Almeida Viana,Karolina Skarlet Silva Caldeira,Patrícia Carlos Abreu,Mauro Henrique Nogueira Guimarães Bernardes,Vanessa Fátima Aguiar,Maria Cássia Ferreira de |
author_sort |
Schuch,Lauren Frenzel |
title |
DNA damage-related proteins in smokers and non-smokers with oral cancer |
title_short |
DNA damage-related proteins in smokers and non-smokers with oral cancer |
title_full |
DNA damage-related proteins in smokers and non-smokers with oral cancer |
title_fullStr |
DNA damage-related proteins in smokers and non-smokers with oral cancer |
title_full_unstemmed |
DNA damage-related proteins in smokers and non-smokers with oral cancer |
title_sort |
dna damage-related proteins in smokers and non-smokers with oral cancer |
description |
Abstract: Tobacco smoking involves a high risk of human malignancies, including oral cancer, because it contains multiple carcinogens that cause genetic instability. Thus, a worse prognosis would be expected for cancer patients who are smokers. The aim of this study was to assess the DNA damage response through the expression of checkpoint kinase 2 (CHK2), H2A histone family member X (H2AX), and P53 among smokers and non-smokers with oral squamous cell carcinoma (OSCC). Associations between immunoexpression of proteins and clinicopathological data and histopathological grading were also analyzed. A total of 35 individuals (18 non-smokers and 17 smokers) with OSCC of the tongue and/or floor of the mouth were included. Immunohistochemistry for H2AX was conducted for the identification of double-strand breaks, CHK2, and P53 to evaluate the expression of this protein in cell cycle regulation. The sample consisted of 22 males and 13 females, with a mean age of 63.9±11.8 years. OSCC of non-smokers were well-differentiated tumors in 50% of the cases, and those of smokers were equally distributed into moderately differentiated and poorly differentiated tumors (35.3% each). Overall, 31 (88.6%) cases were CHK2-positive, 27 (77.1%) were H2AX-positive, and 23 (65.7%) were P53-positive, with no difference between smokers and non-smokers (p > 0.05). No association was found between proteins and clinicopathologic data (p > 0.05). Similarities in CHK2, H2AX, and P53 immunohistochemical staining patterns were observed between smokers and non-smokers, and immunoexpression was not associated with clinicopathological parameters. However, the findings indicated consistent expression of these proteins in OSCC. |
publisher |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publishDate |
2022 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100221 |
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