DNA damage-related proteins in smokers and non-smokers with oral cancer

Abstract: Tobacco smoking involves a high risk of human malignancies, including oral cancer, because it contains multiple carcinogens that cause genetic instability. Thus, a worse prognosis would be expected for cancer patients who are smokers. The aim of this study was to assess the DNA damage response through the expression of checkpoint kinase 2 (CHK2), H2A histone family member X (H2AX), and P53 among smokers and non-smokers with oral squamous cell carcinoma (OSCC). Associations between immunoexpression of proteins and clinicopathological data and histopathological grading were also analyzed. A total of 35 individuals (18 non-smokers and 17 smokers) with OSCC of the tongue and/or floor of the mouth were included. Immunohistochemistry for H2AX was conducted for the identification of double-strand breaks, CHK2, and P53 to evaluate the expression of this protein in cell cycle regulation. The sample consisted of 22 males and 13 females, with a mean age of 63.9±11.8 years. OSCC of non-smokers were well-differentiated tumors in 50% of the cases, and those of smokers were equally distributed into moderately differentiated and poorly differentiated tumors (35.3% each). Overall, 31 (88.6%) cases were CHK2-positive, 27 (77.1%) were H2AX-positive, and 23 (65.7%) were P53-positive, with no difference between smokers and non-smokers (p > 0.05). No association was found between proteins and clinicopathologic data (p > 0.05). Similarities in CHK2, H2AX, and P53 immunohistochemical staining patterns were observed between smokers and non-smokers, and immunoexpression was not associated with clinicopathological parameters. However, the findings indicated consistent expression of these proteins in OSCC.

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Main Authors: Schuch,Lauren Frenzel, de Arruda,José Alcides Almeida, Viana,Karolina Skarlet Silva, Caldeira,Patrícia Carlos, Abreu,Mauro Henrique Nogueira Guimarães, Bernardes,Vanessa Fátima, Aguiar,Maria Cássia Ferreira de
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Pesquisa Odontológica - SBPqO 2022
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100221
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spelling oai:scielo:S1806-832420220001002212022-03-17DNA damage-related proteins in smokers and non-smokers with oral cancerSchuch,Lauren Frenzelde Arruda,José Alcides AlmeidaViana,Karolina Skarlet SilvaCaldeira,Patrícia CarlosAbreu,Mauro Henrique Nogueira GuimarãesBernardes,Vanessa FátimaAguiar,Maria Cássia Ferreira de DNA Damage Checkpoint Kinase 2 Tumor Suppressor Protein p53 Mouth Neoplasms Abstract: Tobacco smoking involves a high risk of human malignancies, including oral cancer, because it contains multiple carcinogens that cause genetic instability. Thus, a worse prognosis would be expected for cancer patients who are smokers. The aim of this study was to assess the DNA damage response through the expression of checkpoint kinase 2 (CHK2), H2A histone family member X (H2AX), and P53 among smokers and non-smokers with oral squamous cell carcinoma (OSCC). Associations between immunoexpression of proteins and clinicopathological data and histopathological grading were also analyzed. A total of 35 individuals (18 non-smokers and 17 smokers) with OSCC of the tongue and/or floor of the mouth were included. Immunohistochemistry for H2AX was conducted for the identification of double-strand breaks, CHK2, and P53 to evaluate the expression of this protein in cell cycle regulation. The sample consisted of 22 males and 13 females, with a mean age of 63.9±11.8 years. OSCC of non-smokers were well-differentiated tumors in 50% of the cases, and those of smokers were equally distributed into moderately differentiated and poorly differentiated tumors (35.3% each). Overall, 31 (88.6%) cases were CHK2-positive, 27 (77.1%) were H2AX-positive, and 23 (65.7%) were P53-positive, with no difference between smokers and non-smokers (p > 0.05). No association was found between proteins and clinicopathologic data (p > 0.05). Similarities in CHK2, H2AX, and P53 immunohistochemical staining patterns were observed between smokers and non-smokers, and immunoexpression was not associated with clinicopathological parameters. However, the findings indicated consistent expression of these proteins in OSCC.info:eu-repo/semantics/openAccessSociedade Brasileira de Pesquisa Odontológica - SBPqOBrazilian Oral Research v.36 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100221en10.1590/1807-3107bor-2022.vol36.0027
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language English
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author Schuch,Lauren Frenzel
de Arruda,José Alcides Almeida
Viana,Karolina Skarlet Silva
Caldeira,Patrícia Carlos
Abreu,Mauro Henrique Nogueira Guimarães
Bernardes,Vanessa Fátima
Aguiar,Maria Cássia Ferreira de
spellingShingle Schuch,Lauren Frenzel
de Arruda,José Alcides Almeida
Viana,Karolina Skarlet Silva
Caldeira,Patrícia Carlos
Abreu,Mauro Henrique Nogueira Guimarães
Bernardes,Vanessa Fátima
Aguiar,Maria Cássia Ferreira de
DNA damage-related proteins in smokers and non-smokers with oral cancer
author_facet Schuch,Lauren Frenzel
de Arruda,José Alcides Almeida
Viana,Karolina Skarlet Silva
Caldeira,Patrícia Carlos
Abreu,Mauro Henrique Nogueira Guimarães
Bernardes,Vanessa Fátima
Aguiar,Maria Cássia Ferreira de
author_sort Schuch,Lauren Frenzel
title DNA damage-related proteins in smokers and non-smokers with oral cancer
title_short DNA damage-related proteins in smokers and non-smokers with oral cancer
title_full DNA damage-related proteins in smokers and non-smokers with oral cancer
title_fullStr DNA damage-related proteins in smokers and non-smokers with oral cancer
title_full_unstemmed DNA damage-related proteins in smokers and non-smokers with oral cancer
title_sort dna damage-related proteins in smokers and non-smokers with oral cancer
description Abstract: Tobacco smoking involves a high risk of human malignancies, including oral cancer, because it contains multiple carcinogens that cause genetic instability. Thus, a worse prognosis would be expected for cancer patients who are smokers. The aim of this study was to assess the DNA damage response through the expression of checkpoint kinase 2 (CHK2), H2A histone family member X (H2AX), and P53 among smokers and non-smokers with oral squamous cell carcinoma (OSCC). Associations between immunoexpression of proteins and clinicopathological data and histopathological grading were also analyzed. A total of 35 individuals (18 non-smokers and 17 smokers) with OSCC of the tongue and/or floor of the mouth were included. Immunohistochemistry for H2AX was conducted for the identification of double-strand breaks, CHK2, and P53 to evaluate the expression of this protein in cell cycle regulation. The sample consisted of 22 males and 13 females, with a mean age of 63.9±11.8 years. OSCC of non-smokers were well-differentiated tumors in 50% of the cases, and those of smokers were equally distributed into moderately differentiated and poorly differentiated tumors (35.3% each). Overall, 31 (88.6%) cases were CHK2-positive, 27 (77.1%) were H2AX-positive, and 23 (65.7%) were P53-positive, with no difference between smokers and non-smokers (p > 0.05). No association was found between proteins and clinicopathologic data (p > 0.05). Similarities in CHK2, H2AX, and P53 immunohistochemical staining patterns were observed between smokers and non-smokers, and immunoexpression was not associated with clinicopathological parameters. However, the findings indicated consistent expression of these proteins in OSCC.
publisher Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publishDate 2022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100221
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