Do different strains of E. faecalis have the same behavior towards intracanal medications in in vitro research?
Abstract: The aim of this study was to evaluate the antimicrobial action of different endodontic pastes against Enterococcus faecalis ATCC 29212, isolated from the urinary tract, and compare the action with E. faecalis ATCC 4083, isolated from the root canal. For this purpose, dentin blocks were infected for 21 days with both bacteria at different time-intervals to ensure there would be no cross contamination. After this period, blocks were immersed in the test medications for 7 days, according to the following groups: CH/S, CH/P, CH/CMCP, CH/CHX, CH/DAP and TAP. Images of the samples were captured with a confocal microscope and the percentage of live cells was computed by means of the Bioimage program. The ATCC 29212 strain was shown to be more resistant to CH/SS, Calen, CH/DAP, and TAP than the ATCC 4083 strain. The antimicrobial action of the medications against each strain were divergent concerning the order of susceptibility. The authors concluded that the strains behaved in a different manner: in general, those extracted from the urinary tract were more resistant to the tested medications. Therefore, when E. faecalis must be used for in vitro research in endodontics, we suggest the use of ATCC 4083 strain to obtain results that are closer to the clinical reality.
Main Authors: | Zancan,Rafaela Fernandes, Canali,Lyz Cristina Furquim, Tartari,Talita, Andrade,Flaviana Bombarda de, Vivan,Rodrigo Ricci, Duarte,Marco Antonio Hungaro |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO
2018
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100238 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Influence of EDTA and its Association with Benzalkonium Chloride on Enterococcus faecalis Adhesion to Dentin
by: Titato,Pedro Cesar Gomes, et al.
Published: (2020) -
Can kinematics, file diameter, and PUI influence the intracanal decontamination and apical bacterial extrusion?
by: Cuellar,Maricel Rosario Cardenas, et al.
Published: (2021) -
Antimicrobial activity of sodium hypochlorite associated with intracanal medication for Candida albicans and Enterococcus faecalis inoculated in root canals
by: Valera,Marcia Carneiro, et al.
Published: (2009) -
Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalis
by: Farac,Roberta Vieira, et al.
Published: (2013) -
Cytotoxicity of intracanal dressings on apical papilla cells differ upon activation with E. faecalis LTA
by: SIPERT,Carla Renata, et al.
Published: (2019)