Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions

Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions

Abstract The objective of this study was to evaluate the expression of matrix metalloproteinase 9 (MMP-9) and transforming growth factor beta (TGF-β1) in periapical lesion samples correlated with the intensity of the inflammatory infiltrate and thickness of the epithelial lining. Forty-five cases of periapical lesions (23 periapical granulomas and 22 radicular cysts) were subjected to morphological and immunohistochemical analyses using anti-MMP-9 and anti-TGF-β1 antibodies. The data were analyzed using the following tests: non-parametric Mann-Whitney, chi-square, Fisher’s exact test and Spearman’s correlation test (P<0.05). Analysis of inflammatory infiltrate revealed that 78% of periapical granulomas presented infiltrate grade III, in contrast with 32% of radicular cysts (P<0.001). Morphological evaluation of the epithelial thickness in radicular cysts revealed the presence of atrophic epithelium in 86% of the cysts. The immunostaining of MMP-9 was score 2 in 67% of the granulomas and 77% of the cysts. Both lesions were predominantly score 1 for TGF-β1. Significant differences were confirmed between the expression scores of TGF-β1 and MMP-9 in periapical granulomas (p = 0.004) and in radicular cysts (p < 0.001). Expression of TGF-β1 was different for periapical granulomas and radicular cysts. This immunoregulatory cytokine seems more representative in asymptomatic lesions. The extracellular matrix remodeling process dependent on MMP-9 seems to be similar for both periapical granulomas and radicular cysts. TGF-β1 and MMP-9 may play an important role in the maintenance of periapical lesions.

Saved in:
Bibliographic Details
Main Authors: ÁLVARES,Pâmella Recco, ARRUDA,José Alcides Almeida de, SILVA,Leorik Pereira da, NASCIMENTO,George João Ferreira do, SILVEIRA,Maria Fonseca da, SOBRAL,Ana Paula Veras
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Pesquisa Odontológica - SBPqO 2017
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100249
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S1806-83242017000100249
record_format ojs
spelling oai:scielo:S1806-832420170001002492017-06-30Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesionsÁLVARES,Pâmella ReccoARRUDA,José Alcides Almeida deSILVA,Leorik Pereira daNASCIMENTO,George João Ferreira doSILVEIRA,Maria Fonseca daSOBRAL,Ana Paula Veras Inflammation Immunohistochemistry Periapical Granuloma Radicular Cyst Biopsy Abstract The objective of this study was to evaluate the expression of matrix metalloproteinase 9 (MMP-9) and transforming growth factor beta (TGF-β1) in periapical lesion samples correlated with the intensity of the inflammatory infiltrate and thickness of the epithelial lining. Forty-five cases of periapical lesions (23 periapical granulomas and 22 radicular cysts) were subjected to morphological and immunohistochemical analyses using anti-MMP-9 and anti-TGF-β1 antibodies. The data were analyzed using the following tests: non-parametric Mann-Whitney, chi-square, Fisher’s exact test and Spearman’s correlation test (P<0.05). Analysis of inflammatory infiltrate revealed that 78% of periapical granulomas presented infiltrate grade III, in contrast with 32% of radicular cysts (P<0.001). Morphological evaluation of the epithelial thickness in radicular cysts revealed the presence of atrophic epithelium in 86% of the cysts. The immunostaining of MMP-9 was score 2 in 67% of the granulomas and 77% of the cysts. Both lesions were predominantly score 1 for TGF-β1. Significant differences were confirmed between the expression scores of TGF-β1 and MMP-9 in periapical granulomas (p = 0.004) and in radicular cysts (p < 0.001). Expression of TGF-β1 was different for periapical granulomas and radicular cysts. This immunoregulatory cytokine seems more representative in asymptomatic lesions. The extracellular matrix remodeling process dependent on MMP-9 seems to be similar for both periapical granulomas and radicular cysts. TGF-β1 and MMP-9 may play an important role in the maintenance of periapical lesions.info:eu-repo/semantics/openAccessSociedade Brasileira de Pesquisa Odontológica - SBPqOBrazilian Oral Research v.31 20172017-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100249en10.1590/1807-3107bor-2017.vol31.0051
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author ÁLVARES,Pâmella Recco
ARRUDA,José Alcides Almeida de
SILVA,Leorik Pereira da
NASCIMENTO,George João Ferreira do
SILVEIRA,Maria Fonseca da
SOBRAL,Ana Paula Veras
spellingShingle ÁLVARES,Pâmella Recco
ARRUDA,José Alcides Almeida de
SILVA,Leorik Pereira da
NASCIMENTO,George João Ferreira do
SILVEIRA,Maria Fonseca da
SOBRAL,Ana Paula Veras
Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions
author_facet ÁLVARES,Pâmella Recco
ARRUDA,José Alcides Almeida de
SILVA,Leorik Pereira da
NASCIMENTO,George João Ferreira do
SILVEIRA,Maria Fonseca da
SOBRAL,Ana Paula Veras
author_sort ÁLVARES,Pâmella Recco
title Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions
title_short Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions
title_full Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions
title_fullStr Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions
title_full_unstemmed Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions
title_sort immunohistochemical expression of tgf-β1 and mmp-9 in periapical lesions
description Abstract The objective of this study was to evaluate the expression of matrix metalloproteinase 9 (MMP-9) and transforming growth factor beta (TGF-β1) in periapical lesion samples correlated with the intensity of the inflammatory infiltrate and thickness of the epithelial lining. Forty-five cases of periapical lesions (23 periapical granulomas and 22 radicular cysts) were subjected to morphological and immunohistochemical analyses using anti-MMP-9 and anti-TGF-β1 antibodies. The data were analyzed using the following tests: non-parametric Mann-Whitney, chi-square, Fisher’s exact test and Spearman’s correlation test (P<0.05). Analysis of inflammatory infiltrate revealed that 78% of periapical granulomas presented infiltrate grade III, in contrast with 32% of radicular cysts (P<0.001). Morphological evaluation of the epithelial thickness in radicular cysts revealed the presence of atrophic epithelium in 86% of the cysts. The immunostaining of MMP-9 was score 2 in 67% of the granulomas and 77% of the cysts. Both lesions were predominantly score 1 for TGF-β1. Significant differences were confirmed between the expression scores of TGF-β1 and MMP-9 in periapical granulomas (p = 0.004) and in radicular cysts (p < 0.001). Expression of TGF-β1 was different for periapical granulomas and radicular cysts. This immunoregulatory cytokine seems more representative in asymptomatic lesions. The extracellular matrix remodeling process dependent on MMP-9 seems to be similar for both periapical granulomas and radicular cysts. TGF-β1 and MMP-9 may play an important role in the maintenance of periapical lesions.
publisher Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publishDate 2017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100249
work_keys_str_mv AT alvarespamellarecco immunohistochemicalexpressionoftgfb1andmmp9inperiapicallesions
AT arrudajosealcidesalmeidade immunohistochemicalexpressionoftgfb1andmmp9inperiapicallesions
AT silvaleorikpereirada immunohistochemicalexpressionoftgfb1andmmp9inperiapicallesions
AT nascimentogeorgejoaoferreirado immunohistochemicalexpressionoftgfb1andmmp9inperiapicallesions
AT silveiramariafonsecada immunohistochemicalexpressionoftgfb1andmmp9inperiapicallesions
AT sobralanapaulaveras immunohistochemicalexpressionoftgfb1andmmp9inperiapicallesions
_version_ 1756431609227116544

Similar Items