Experimental osteonecrosis: development of a model in rodents administered alendronate
Abstract The main objective of this study was to cause bisphosphonate-related osteonecrosis of the jaws to develop in a rodent model. Adult male Holtzman rats were assigned to one of two experimental groups to receive alendronate (AL; 1 mg/kg/week; n = 6) or saline solution (CTL; n = 6). After 60 days of drug therapy, all animals were subjected to first lower molar extraction, and 28 days later, animals were euthanized. All rats treated with alendronate developed osteonecrosis, presenting as ulcers and necrotic bone, associated with a significant infection process, especially at the inter-alveolar septum area and crestal regions. The degree of vascularization, the levels of C-telopeptide cross-linked collagen type I and bone-specific alkaline phosphatase, as well as the bone volume were significantly reduced in these animals. Furthermore, on radiographic analysis, animals treated with alendronate presented evident sclerosis of the lamina dura of the lower first molar alveolar socket associated with decreased radiographic density in this area. These findings indicate that the protocol developed in the present study opens new perspectives and could be a good starting model for future property design.
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Sociedade Brasileira de Pesquisa Odontológica - SBPqO
2016
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oai:scielo:S1806-832420160001002852016-08-16Experimental osteonecrosis: development of a model in rodents administered alendronateCONTE NETO,NicolauSPOLIDORIO,Luis CarlosANDRADE,Cleverton Roberto deESTEVES,Jônatas CaldeiraMARCANTONIO JR,Elcio Osteonecrosis Alendronate Infection Abstract The main objective of this study was to cause bisphosphonate-related osteonecrosis of the jaws to develop in a rodent model. Adult male Holtzman rats were assigned to one of two experimental groups to receive alendronate (AL; 1 mg/kg/week; n = 6) or saline solution (CTL; n = 6). After 60 days of drug therapy, all animals were subjected to first lower molar extraction, and 28 days later, animals were euthanized. All rats treated with alendronate developed osteonecrosis, presenting as ulcers and necrotic bone, associated with a significant infection process, especially at the inter-alveolar septum area and crestal regions. The degree of vascularization, the levels of C-telopeptide cross-linked collagen type I and bone-specific alkaline phosphatase, as well as the bone volume were significantly reduced in these animals. Furthermore, on radiographic analysis, animals treated with alendronate presented evident sclerosis of the lamina dura of the lower first molar alveolar socket associated with decreased radiographic density in this area. These findings indicate that the protocol developed in the present study opens new perspectives and could be a good starting model for future property design.info:eu-repo/semantics/openAccessSociedade Brasileira de Pesquisa Odontológica - SBPqOBrazilian Oral Research v.30 n.1 20162016-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100285en10.1590/1807-3107BOR-2016.vol30.0099 |
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CONTE NETO,Nicolau SPOLIDORIO,Luis Carlos ANDRADE,Cleverton Roberto de ESTEVES,Jônatas Caldeira MARCANTONIO JR,Elcio |
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CONTE NETO,Nicolau SPOLIDORIO,Luis Carlos ANDRADE,Cleverton Roberto de ESTEVES,Jônatas Caldeira MARCANTONIO JR,Elcio Experimental osteonecrosis: development of a model in rodents administered alendronate |
author_facet |
CONTE NETO,Nicolau SPOLIDORIO,Luis Carlos ANDRADE,Cleverton Roberto de ESTEVES,Jônatas Caldeira MARCANTONIO JR,Elcio |
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CONTE NETO,Nicolau |
title |
Experimental osteonecrosis: development of a model in rodents administered alendronate |
title_short |
Experimental osteonecrosis: development of a model in rodents administered alendronate |
title_full |
Experimental osteonecrosis: development of a model in rodents administered alendronate |
title_fullStr |
Experimental osteonecrosis: development of a model in rodents administered alendronate |
title_full_unstemmed |
Experimental osteonecrosis: development of a model in rodents administered alendronate |
title_sort |
experimental osteonecrosis: development of a model in rodents administered alendronate |
description |
Abstract The main objective of this study was to cause bisphosphonate-related osteonecrosis of the jaws to develop in a rodent model. Adult male Holtzman rats were assigned to one of two experimental groups to receive alendronate (AL; 1 mg/kg/week; n = 6) or saline solution (CTL; n = 6). After 60 days of drug therapy, all animals were subjected to first lower molar extraction, and 28 days later, animals were euthanized. All rats treated with alendronate developed osteonecrosis, presenting as ulcers and necrotic bone, associated with a significant infection process, especially at the inter-alveolar septum area and crestal regions. The degree of vascularization, the levels of C-telopeptide cross-linked collagen type I and bone-specific alkaline phosphatase, as well as the bone volume were significantly reduced in these animals. Furthermore, on radiographic analysis, animals treated with alendronate presented evident sclerosis of the lamina dura of the lower first molar alveolar socket associated with decreased radiographic density in this area. These findings indicate that the protocol developed in the present study opens new perspectives and could be a good starting model for future property design. |
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Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
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2016 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100285 |
work_keys_str_mv |
AT contenetonicolau experimentalosteonecrosisdevelopmentofamodelinrodentsadministeredalendronate AT spolidorioluiscarlos experimentalosteonecrosisdevelopmentofamodelinrodentsadministeredalendronate AT andradeclevertonrobertode experimentalosteonecrosisdevelopmentofamodelinrodentsadministeredalendronate AT estevesjonatascaldeira experimentalosteonecrosisdevelopmentofamodelinrodentsadministeredalendronate AT marcantoniojrelcio experimentalosteonecrosisdevelopmentofamodelinrodentsadministeredalendronate |
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