Biofilm biomass disruption by natural substances with potential for endodontic use
This study evaluated the in vitro effects of four natural substances on the biomass of bacterial biofilms to assess their potential use as root canal irrigants. The following substances and their combinations were tested: 0.2% farnesol; 5% xylitol; 20% xylitol; 0.2% farnesol and 5% xylitol; 0.2% farnesol, 5% xylitol, and 0.1% lactoferrin; 5% xylitol and 0.1% lactoferrin; and 20 mM salicylic acid. The crystal violet assay was used to evaluate the effects of these substances on the biomass of biofilms formed by Enterococcus faecalis and Staphylococcus epidermidis. All substances except for 20 mM salicylic acid and 20% xylitol reduced biofilm mass when compared to controls. The combination of farnesol and xylitol was the most effective agent against E. faecalis ATCC 29212 (p < 0.05). Farnesol combined with xylitol and lactoferrin was the most effective against biofilms of the endodontic strain of E. faecalis MB35 (p < 0.05). Similarly, combinations involving farnesol, xylitol, and lactoferrin reduced the biomass of S. epidermidis biofilms. In general, farnesol, xylitol, and lactoferrin or farnesol and xylitol reduced biofilm biomass most effectively. Therefore, it was concluded that combinations of antibiofilm substances have potential use in endodontic treatment to combat biofilms.
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Sociedade Brasileira de Pesquisa Odontológica - SBPqO
2013
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oai:scielo:S1806-832420130001000042015-08-26Biofilm biomass disruption by natural substances with potential for endodontic useAlves,Flávio Rodrigues FerreiraSilva,Marlei GomesRôças,Isabela NevesSiqueira Jr,José Freitas Farnesol Xylitol Lactoferrin Enterococcus faecalis This study evaluated the in vitro effects of four natural substances on the biomass of bacterial biofilms to assess their potential use as root canal irrigants. The following substances and their combinations were tested: 0.2% farnesol; 5% xylitol; 20% xylitol; 0.2% farnesol and 5% xylitol; 0.2% farnesol, 5% xylitol, and 0.1% lactoferrin; 5% xylitol and 0.1% lactoferrin; and 20 mM salicylic acid. The crystal violet assay was used to evaluate the effects of these substances on the biomass of biofilms formed by Enterococcus faecalis and Staphylococcus epidermidis. All substances except for 20 mM salicylic acid and 20% xylitol reduced biofilm mass when compared to controls. The combination of farnesol and xylitol was the most effective agent against E. faecalis ATCC 29212 (p < 0.05). Farnesol combined with xylitol and lactoferrin was the most effective against biofilms of the endodontic strain of E. faecalis MB35 (p < 0.05). Similarly, combinations involving farnesol, xylitol, and lactoferrin reduced the biomass of S. epidermidis biofilms. In general, farnesol, xylitol, and lactoferrin or farnesol and xylitol reduced biofilm biomass most effectively. Therefore, it was concluded that combinations of antibiofilm substances have potential use in endodontic treatment to combat biofilms.info:eu-repo/semantics/openAccessSociedade Brasileira de Pesquisa Odontológica - SBPqOBrazilian Oral Research v.27 n.1 20132013-02-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242013000100004en10.1590/S1806-83242013000100004 |
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Alves,Flávio Rodrigues Ferreira Silva,Marlei Gomes Rôças,Isabela Neves Siqueira Jr,José Freitas |
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Alves,Flávio Rodrigues Ferreira Silva,Marlei Gomes Rôças,Isabela Neves Siqueira Jr,José Freitas Biofilm biomass disruption by natural substances with potential for endodontic use |
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Alves,Flávio Rodrigues Ferreira Silva,Marlei Gomes Rôças,Isabela Neves Siqueira Jr,José Freitas |
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Alves,Flávio Rodrigues Ferreira |
title |
Biofilm biomass disruption by natural substances with potential for endodontic use |
title_short |
Biofilm biomass disruption by natural substances with potential for endodontic use |
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Biofilm biomass disruption by natural substances with potential for endodontic use |
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Biofilm biomass disruption by natural substances with potential for endodontic use |
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Biofilm biomass disruption by natural substances with potential for endodontic use |
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biofilm biomass disruption by natural substances with potential for endodontic use |
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This study evaluated the in vitro effects of four natural substances on the biomass of bacterial biofilms to assess their potential use as root canal irrigants. The following substances and their combinations were tested: 0.2% farnesol; 5% xylitol; 20% xylitol; 0.2% farnesol and 5% xylitol; 0.2% farnesol, 5% xylitol, and 0.1% lactoferrin; 5% xylitol and 0.1% lactoferrin; and 20 mM salicylic acid. The crystal violet assay was used to evaluate the effects of these substances on the biomass of biofilms formed by Enterococcus faecalis and Staphylococcus epidermidis. All substances except for 20 mM salicylic acid and 20% xylitol reduced biofilm mass when compared to controls. The combination of farnesol and xylitol was the most effective agent against E. faecalis ATCC 29212 (p < 0.05). Farnesol combined with xylitol and lactoferrin was the most effective against biofilms of the endodontic strain of E. faecalis MB35 (p < 0.05). Similarly, combinations involving farnesol, xylitol, and lactoferrin reduced the biomass of S. epidermidis biofilms. In general, farnesol, xylitol, and lactoferrin or farnesol and xylitol reduced biofilm biomass most effectively. Therefore, it was concluded that combinations of antibiofilm substances have potential use in endodontic treatment to combat biofilms. |
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Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
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2013 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242013000100004 |
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