Biochemical changes in hybrid pumpkin seeds at different stages of maturation
ABSTRACT This study aimed to evaluate biochemical changes in seeds of the pumpkin hybrid, 'Jabras', from fruit harvested at different stages of maturation (15, 30, 45, 60 and 75 days after anthesis). Thirty fruit were harvested at each stage, with the seeds from 15 of the fruit being extracted immediately. The remaining 15 were stored for twenty days in plastic boxes and the seeds extracted after this period. After processing and drying the seeds, the following were determined: moisture content, germination, first count and antioxidant enzyme activity (peroxidase, ascorbate peroxidase, catalase and superoxide dismutase). Seeds from the fruit harvested at 30 DAA displayed low values for germination and vigour and high antioxidant enzyme activity, indicating that they were immature and that drying possibly caused damage to the system of cell membranes. The results obtained in this study demonstrated that analysis related to changes in the activity of enzymes during development and maturation of the seeds was effective in evaluating the physiological and biochemical changes in pumpkin seeds of the 'Jabras' cultivar.
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Universidade Federal do Ceará
2017
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oai:scielo:S1806-669020170003005582017-05-22Biochemical changes in hybrid pumpkin seeds at different stages of maturationSilva,Patrícia Pereira daBarros,Antonio Carlos Souza AlbuquerqueDias,Denise Cunha Fernandes dos SantosSekita,Marcelo CoelhoNascimento,Warley Marcos Cucurbita maxima Cucurbita moschata Antioxidant enzymes ABSTRACT This study aimed to evaluate biochemical changes in seeds of the pumpkin hybrid, 'Jabras', from fruit harvested at different stages of maturation (15, 30, 45, 60 and 75 days after anthesis). Thirty fruit were harvested at each stage, with the seeds from 15 of the fruit being extracted immediately. The remaining 15 were stored for twenty days in plastic boxes and the seeds extracted after this period. After processing and drying the seeds, the following were determined: moisture content, germination, first count and antioxidant enzyme activity (peroxidase, ascorbate peroxidase, catalase and superoxide dismutase). Seeds from the fruit harvested at 30 DAA displayed low values for germination and vigour and high antioxidant enzyme activity, indicating that they were immature and that drying possibly caused damage to the system of cell membranes. The results obtained in this study demonstrated that analysis related to changes in the activity of enzymes during development and maturation of the seeds was effective in evaluating the physiological and biochemical changes in pumpkin seeds of the 'Jabras' cultivar.info:eu-repo/semantics/openAccessUniversidade Federal do CearáRevista Ciência Agronômica v.48 n.3 20172017-07-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000300558en10.5935/1806-6690.20170065 |
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Silva,Patrícia Pereira da Barros,Antonio Carlos Souza Albuquerque Dias,Denise Cunha Fernandes dos Santos Sekita,Marcelo Coelho Nascimento,Warley Marcos |
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Silva,Patrícia Pereira da Barros,Antonio Carlos Souza Albuquerque Dias,Denise Cunha Fernandes dos Santos Sekita,Marcelo Coelho Nascimento,Warley Marcos Biochemical changes in hybrid pumpkin seeds at different stages of maturation |
author_facet |
Silva,Patrícia Pereira da Barros,Antonio Carlos Souza Albuquerque Dias,Denise Cunha Fernandes dos Santos Sekita,Marcelo Coelho Nascimento,Warley Marcos |
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Silva,Patrícia Pereira da |
title |
Biochemical changes in hybrid pumpkin seeds at different stages of maturation |
title_short |
Biochemical changes in hybrid pumpkin seeds at different stages of maturation |
title_full |
Biochemical changes in hybrid pumpkin seeds at different stages of maturation |
title_fullStr |
Biochemical changes in hybrid pumpkin seeds at different stages of maturation |
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Biochemical changes in hybrid pumpkin seeds at different stages of maturation |
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biochemical changes in hybrid pumpkin seeds at different stages of maturation |
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ABSTRACT This study aimed to evaluate biochemical changes in seeds of the pumpkin hybrid, 'Jabras', from fruit harvested at different stages of maturation (15, 30, 45, 60 and 75 days after anthesis). Thirty fruit were harvested at each stage, with the seeds from 15 of the fruit being extracted immediately. The remaining 15 were stored for twenty days in plastic boxes and the seeds extracted after this period. After processing and drying the seeds, the following were determined: moisture content, germination, first count and antioxidant enzyme activity (peroxidase, ascorbate peroxidase, catalase and superoxide dismutase). Seeds from the fruit harvested at 30 DAA displayed low values for germination and vigour and high antioxidant enzyme activity, indicating that they were immature and that drying possibly caused damage to the system of cell membranes. The results obtained in this study demonstrated that analysis related to changes in the activity of enzymes during development and maturation of the seeds was effective in evaluating the physiological and biochemical changes in pumpkin seeds of the 'Jabras' cultivar. |
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Universidade Federal do Ceará |
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2017 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000300558 |
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1756431378810929152 |