Biochemical changes in hybrid pumpkin seeds at different stages of maturation

ABSTRACT This study aimed to evaluate biochemical changes in seeds of the pumpkin hybrid, 'Jabras', from fruit harvested at different stages of maturation (15, 30, 45, 60 and 75 days after anthesis). Thirty fruit were harvested at each stage, with the seeds from 15 of the fruit being extracted immediately. The remaining 15 were stored for twenty days in plastic boxes and the seeds extracted after this period. After processing and drying the seeds, the following were determined: moisture content, germination, first count and antioxidant enzyme activity (peroxidase, ascorbate peroxidase, catalase and superoxide dismutase). Seeds from the fruit harvested at 30 DAA displayed low values for germination and vigour and high antioxidant enzyme activity, indicating that they were immature and that drying possibly caused damage to the system of cell membranes. The results obtained in this study demonstrated that analysis related to changes in the activity of enzymes during development and maturation of the seeds was effective in evaluating the physiological and biochemical changes in pumpkin seeds of the 'Jabras' cultivar.

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Main Authors: Silva,Patrícia Pereira da, Barros,Antonio Carlos Souza Albuquerque, Dias,Denise Cunha Fernandes dos Santos, Sekita,Marcelo Coelho, Nascimento,Warley Marcos
Format: Digital revista
Language:English
Published: Universidade Federal do Ceará 2017
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000300558
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spelling oai:scielo:S1806-669020170003005582017-05-22Biochemical changes in hybrid pumpkin seeds at different stages of maturationSilva,Patrícia Pereira daBarros,Antonio Carlos Souza AlbuquerqueDias,Denise Cunha Fernandes dos SantosSekita,Marcelo CoelhoNascimento,Warley Marcos Cucurbita maxima Cucurbita moschata Antioxidant enzymes ABSTRACT This study aimed to evaluate biochemical changes in seeds of the pumpkin hybrid, 'Jabras', from fruit harvested at different stages of maturation (15, 30, 45, 60 and 75 days after anthesis). Thirty fruit were harvested at each stage, with the seeds from 15 of the fruit being extracted immediately. The remaining 15 were stored for twenty days in plastic boxes and the seeds extracted after this period. After processing and drying the seeds, the following were determined: moisture content, germination, first count and antioxidant enzyme activity (peroxidase, ascorbate peroxidase, catalase and superoxide dismutase). Seeds from the fruit harvested at 30 DAA displayed low values for germination and vigour and high antioxidant enzyme activity, indicating that they were immature and that drying possibly caused damage to the system of cell membranes. The results obtained in this study demonstrated that analysis related to changes in the activity of enzymes during development and maturation of the seeds was effective in evaluating the physiological and biochemical changes in pumpkin seeds of the 'Jabras' cultivar.info:eu-repo/semantics/openAccessUniversidade Federal do CearáRevista Ciência Agronômica v.48 n.3 20172017-07-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000300558en10.5935/1806-6690.20170065
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Silva,Patrícia Pereira da
Barros,Antonio Carlos Souza Albuquerque
Dias,Denise Cunha Fernandes dos Santos
Sekita,Marcelo Coelho
Nascimento,Warley Marcos
spellingShingle Silva,Patrícia Pereira da
Barros,Antonio Carlos Souza Albuquerque
Dias,Denise Cunha Fernandes dos Santos
Sekita,Marcelo Coelho
Nascimento,Warley Marcos
Biochemical changes in hybrid pumpkin seeds at different stages of maturation
author_facet Silva,Patrícia Pereira da
Barros,Antonio Carlos Souza Albuquerque
Dias,Denise Cunha Fernandes dos Santos
Sekita,Marcelo Coelho
Nascimento,Warley Marcos
author_sort Silva,Patrícia Pereira da
title Biochemical changes in hybrid pumpkin seeds at different stages of maturation
title_short Biochemical changes in hybrid pumpkin seeds at different stages of maturation
title_full Biochemical changes in hybrid pumpkin seeds at different stages of maturation
title_fullStr Biochemical changes in hybrid pumpkin seeds at different stages of maturation
title_full_unstemmed Biochemical changes in hybrid pumpkin seeds at different stages of maturation
title_sort biochemical changes in hybrid pumpkin seeds at different stages of maturation
description ABSTRACT This study aimed to evaluate biochemical changes in seeds of the pumpkin hybrid, 'Jabras', from fruit harvested at different stages of maturation (15, 30, 45, 60 and 75 days after anthesis). Thirty fruit were harvested at each stage, with the seeds from 15 of the fruit being extracted immediately. The remaining 15 were stored for twenty days in plastic boxes and the seeds extracted after this period. After processing and drying the seeds, the following were determined: moisture content, germination, first count and antioxidant enzyme activity (peroxidase, ascorbate peroxidase, catalase and superoxide dismutase). Seeds from the fruit harvested at 30 DAA displayed low values for germination and vigour and high antioxidant enzyme activity, indicating that they were immature and that drying possibly caused damage to the system of cell membranes. The results obtained in this study demonstrated that analysis related to changes in the activity of enzymes during development and maturation of the seeds was effective in evaluating the physiological and biochemical changes in pumpkin seeds of the 'Jabras' cultivar.
publisher Universidade Federal do Ceará
publishDate 2017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000300558
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