Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study
OBJECTIVE: To determine whether tramadol has a protective effect against lung injury induced by skeletal muscle ischemia-reperfusion. METHODS: Twenty Wistar male rats were allocated to one of two groups: ischemia-reperfusion (IR) and ischemia-reperfusion + tramadol (IR+T). The animals were anesthetized with intramuscular injections of ketamine and xylazine (50 mg/kg and 10 mg/kg, respectively). All of the animals underwent 2-h ischemia by occlusion of the femoral artery and 24-h reperfusion. Prior to the occlusion of the femoral artery, 250 IU heparin were administered via the jugular vein in order to prevent clotting. The rats in the IR+T group were treated with tramadol (20 mg/kg i.v.) immediately before reperfusion. After the reperfusion period, the animals were euthanized with pentobarbital (300 mg/kg i.p.), the lungs were carefully removed, and specimens were properly prepared for histopathological and biochemical studies. RESULTS: Myeloperoxidase activity and nitric oxide levels were significantly higher in the IR group than in the IR+T group (p = 0.001 for both). Histological abnormalities, such as intra-alveolar edema, intra-alveolar hemorrhage, and neutrophil infiltration, were significantly more common in the IR group than in the IR+T group. CONCLUSIONS: On the basis of our histological and biochemical findings, we conclude that tramadol prevents lung tissue injury after skeletal muscle ischemia-reperfusion.
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Sociedade Brasileira de Pneumologia e Tisiologia
2013
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oai:scielo:S1806-371320130004004342013-10-08Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental studyTakhtfooladi,Mohammad AshrafzadehJahanshahi,AmiraliSotoudeh,AmirJahanshahi,GholamrezaTakhtfooladi,Hamed AshrafzadehAslani,Kimia Tramadol Muscle, skeletal Ischemic attack, transient Lung Injury OBJECTIVE: To determine whether tramadol has a protective effect against lung injury induced by skeletal muscle ischemia-reperfusion. METHODS: Twenty Wistar male rats were allocated to one of two groups: ischemia-reperfusion (IR) and ischemia-reperfusion + tramadol (IR+T). The animals were anesthetized with intramuscular injections of ketamine and xylazine (50 mg/kg and 10 mg/kg, respectively). All of the animals underwent 2-h ischemia by occlusion of the femoral artery and 24-h reperfusion. Prior to the occlusion of the femoral artery, 250 IU heparin were administered via the jugular vein in order to prevent clotting. The rats in the IR+T group were treated with tramadol (20 mg/kg i.v.) immediately before reperfusion. After the reperfusion period, the animals were euthanized with pentobarbital (300 mg/kg i.p.), the lungs were carefully removed, and specimens were properly prepared for histopathological and biochemical studies. RESULTS: Myeloperoxidase activity and nitric oxide levels were significantly higher in the IR group than in the IR+T group (p = 0.001 for both). Histological abnormalities, such as intra-alveolar edema, intra-alveolar hemorrhage, and neutrophil infiltration, were significantly more common in the IR group than in the IR+T group. CONCLUSIONS: On the basis of our histological and biochemical findings, we conclude that tramadol prevents lung tissue injury after skeletal muscle ischemia-reperfusion. info:eu-repo/semantics/openAccessSociedade Brasileira de Pneumologia e TisiologiaJornal Brasileiro de Pneumologia v.39 n.4 20132013-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132013000400434en10.1590/S1806-37132013000400006 |
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Takhtfooladi,Mohammad Ashrafzadeh Jahanshahi,Amirali Sotoudeh,Amir Jahanshahi,Gholamreza Takhtfooladi,Hamed Ashrafzadeh Aslani,Kimia |
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Takhtfooladi,Mohammad Ashrafzadeh Jahanshahi,Amirali Sotoudeh,Amir Jahanshahi,Gholamreza Takhtfooladi,Hamed Ashrafzadeh Aslani,Kimia Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study |
author_facet |
Takhtfooladi,Mohammad Ashrafzadeh Jahanshahi,Amirali Sotoudeh,Amir Jahanshahi,Gholamreza Takhtfooladi,Hamed Ashrafzadeh Aslani,Kimia |
author_sort |
Takhtfooladi,Mohammad Ashrafzadeh |
title |
Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study |
title_short |
Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study |
title_full |
Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study |
title_fullStr |
Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study |
title_full_unstemmed |
Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study |
title_sort |
effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study |
description |
OBJECTIVE: To determine whether tramadol has a protective effect against lung injury induced by skeletal muscle ischemia-reperfusion. METHODS: Twenty Wistar male rats were allocated to one of two groups: ischemia-reperfusion (IR) and ischemia-reperfusion + tramadol (IR+T). The animals were anesthetized with intramuscular injections of ketamine and xylazine (50 mg/kg and 10 mg/kg, respectively). All of the animals underwent 2-h ischemia by occlusion of the femoral artery and 24-h reperfusion. Prior to the occlusion of the femoral artery, 250 IU heparin were administered via the jugular vein in order to prevent clotting. The rats in the IR+T group were treated with tramadol (20 mg/kg i.v.) immediately before reperfusion. After the reperfusion period, the animals were euthanized with pentobarbital (300 mg/kg i.p.), the lungs were carefully removed, and specimens were properly prepared for histopathological and biochemical studies. RESULTS: Myeloperoxidase activity and nitric oxide levels were significantly higher in the IR group than in the IR+T group (p = 0.001 for both). Histological abnormalities, such as intra-alveolar edema, intra-alveolar hemorrhage, and neutrophil infiltration, were significantly more common in the IR group than in the IR+T group. CONCLUSIONS: On the basis of our histological and biochemical findings, we conclude that tramadol prevents lung tissue injury after skeletal muscle ischemia-reperfusion. |
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Sociedade Brasileira de Pneumologia e Tisiologia |
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2013 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132013000400434 |
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