Water pH and hardness alter ATPases and oxidative stress in the gills and kidney of pacu ( Piaractus mesopotamicus )

Water pH and hardness alter ATPases and oxidative stress in the gills and kidney of pacu ( Piaractus mesopotamicus )

ABSTRACT This study aimed to assess the effects of low and high water hardness in interaction with different water pH in pacu (Piaractus mesopotamicus). Pacu juveniles were subjected to low (50 mg CaCO3 L-1 - LWH) or high water hardness (120 mg CaCO3 L-1 - HWH) at water pH of 5.5 (acidic), 7.5 (circumneutral) or 9.0 (alkaline) for 15 days. Gills and kidneys were collected (days 1, 5 and 15). Gill Na+/K+-ATPase (NKA) and vacuolar-type H+-ATPase (V-ATPase) activities were higher in alkaline pH with HWH on day 1. Gill and kidney NKA and V-ATPase activities were higher in acidic pH with LWH on day 15. Gill NKA activity of pacus under alkaline pH with LWH was higher than those exposed to HWH. Reduced antioxidant capacity in the gills and kidney and enhanced thiobarbituric acid reactive substances (TBARS) levels were demonstrated in fish exposed to acidic or alkaline pH, mainly with LWH. HWH increased glutathione-S-transferase (GST) activity and reduced TBARS levels in the gills and kidney. On day 15, GST activity was increased at acidic pH with LWH. In conclusion, circumneutral pH presents less oxidative stress and fewer variations in ATPases and HWH reduced deleterious effects in fish exposed to acidic or alkaline pH.

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Main Authors: Copatti,Carlos Eduardo, Baldisserotto,Bernardo, Souza,Carine de Freitas, Monserrat,José María, Garcia,Luciano
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Ictiologia 2019
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000500204
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spelling oai:scielo:S1679-622520190005002042019-12-04Water pH and hardness alter ATPases and oxidative stress in the gills and kidney of pacu ( Piaractus mesopotamicus )Copatti,Carlos EduardoBaldisserotto,BernardoSouza,Carine de FreitasMonserrat,José MaríaGarcia,Luciano Acidic pH Alkaline pH GST Na+/K+-ATPase TBARS ABSTRACT This study aimed to assess the effects of low and high water hardness in interaction with different water pH in pacu (Piaractus mesopotamicus). Pacu juveniles were subjected to low (50 mg CaCO3 L-1 - LWH) or high water hardness (120 mg CaCO3 L-1 - HWH) at water pH of 5.5 (acidic), 7.5 (circumneutral) or 9.0 (alkaline) for 15 days. Gills and kidneys were collected (days 1, 5 and 15). Gill Na+/K+-ATPase (NKA) and vacuolar-type H+-ATPase (V-ATPase) activities were higher in alkaline pH with HWH on day 1. Gill and kidney NKA and V-ATPase activities were higher in acidic pH with LWH on day 15. Gill NKA activity of pacus under alkaline pH with LWH was higher than those exposed to HWH. Reduced antioxidant capacity in the gills and kidney and enhanced thiobarbituric acid reactive substances (TBARS) levels were demonstrated in fish exposed to acidic or alkaline pH, mainly with LWH. HWH increased glutathione-S-transferase (GST) activity and reduced TBARS levels in the gills and kidney. On day 15, GST activity was increased at acidic pH with LWH. In conclusion, circumneutral pH presents less oxidative stress and fewer variations in ATPases and HWH reduced deleterious effects in fish exposed to acidic or alkaline pH.info:eu-repo/semantics/openAccessSociedade Brasileira de IctiologiaNeotropical Ichthyology v.17 n.4 20192019-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000500204en10.1590/1982-0224-20190032
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Copatti,Carlos Eduardo
Baldisserotto,Bernardo
Souza,Carine de Freitas
Monserrat,José María
Garcia,Luciano
spellingShingle Copatti,Carlos Eduardo
Baldisserotto,Bernardo
Souza,Carine de Freitas
Monserrat,José María
Garcia,Luciano
Water pH and hardness alter ATPases and oxidative stress in the gills and kidney of pacu ( Piaractus mesopotamicus )
author_facet Copatti,Carlos Eduardo
Baldisserotto,Bernardo
Souza,Carine de Freitas
Monserrat,José María
Garcia,Luciano
author_sort Copatti,Carlos Eduardo
title Water pH and hardness alter ATPases and oxidative stress in the gills and kidney of pacu ( Piaractus mesopotamicus )
title_short Water pH and hardness alter ATPases and oxidative stress in the gills and kidney of pacu ( Piaractus mesopotamicus )
title_full Water pH and hardness alter ATPases and oxidative stress in the gills and kidney of pacu ( Piaractus mesopotamicus )
title_fullStr Water pH and hardness alter ATPases and oxidative stress in the gills and kidney of pacu ( Piaractus mesopotamicus )
title_full_unstemmed Water pH and hardness alter ATPases and oxidative stress in the gills and kidney of pacu ( Piaractus mesopotamicus )
title_sort water ph and hardness alter atpases and oxidative stress in the gills and kidney of pacu ( piaractus mesopotamicus )
description ABSTRACT This study aimed to assess the effects of low and high water hardness in interaction with different water pH in pacu (Piaractus mesopotamicus). Pacu juveniles were subjected to low (50 mg CaCO3 L-1 - LWH) or high water hardness (120 mg CaCO3 L-1 - HWH) at water pH of 5.5 (acidic), 7.5 (circumneutral) or 9.0 (alkaline) for 15 days. Gills and kidneys were collected (days 1, 5 and 15). Gill Na+/K+-ATPase (NKA) and vacuolar-type H+-ATPase (V-ATPase) activities were higher in alkaline pH with HWH on day 1. Gill and kidney NKA and V-ATPase activities were higher in acidic pH with LWH on day 15. Gill NKA activity of pacus under alkaline pH with LWH was higher than those exposed to HWH. Reduced antioxidant capacity in the gills and kidney and enhanced thiobarbituric acid reactive substances (TBARS) levels were demonstrated in fish exposed to acidic or alkaline pH, mainly with LWH. HWH increased glutathione-S-transferase (GST) activity and reduced TBARS levels in the gills and kidney. On day 15, GST activity was increased at acidic pH with LWH. In conclusion, circumneutral pH presents less oxidative stress and fewer variations in ATPases and HWH reduced deleterious effects in fish exposed to acidic or alkaline pH.
publisher Sociedade Brasileira de Ictiologia
publishDate 2019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000500204
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