Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venom
Abstract Background Hadruroides lunatus is the most abundant scorpion species in the Peruvian central coast, where most of the accidents involving humans are registered. In spite of its prevalence, there are only very few studies on H. lunatus envenomation. The aim of the present study was to analyze the cardiorespiratory alterations caused by H. lunatus envenomation in rodents. Methods Wistar rats injected with H. lunatus scorpion venom were submitted to electrocardiography. After euthanasia, rat lungs were collected and histopathologically analyzed. Mouse cardiomyocytes were used to perform immunofluorescence and calcium transient assays. Data were analyzed by ANOVA or Student’s t-test. The significance level was set at p< 0.05. Results It was observed that H. lunatus venom increased heart rate and caused arrhythmia, thereby impairing the heart functioning. Lungs of envenomed animals showed significant alterations, such as diffuse hemorrhage. In addition, immunofluorescence showed that H. lunatus venom was capable of binding to cardiomyocytes. Furthermore, mouse ventricular cardiomyocytes incubated with H. lunatus venom showed a significant decrease in calcium transient, confirming that H. lunatus venom exerts a toxic effect on heart. Conclusion Our results showed that H. lunatus venom is capable of inducing cardiorespiratory alterations, a typical systemic effect of scorpionism, stressing the importance of medical monitoring in envenomation cases.
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Digital revista |
| Language: | English |
| Published: |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
2017
|
| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100301 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
oai:scielo:S1678-91992017000100301 |
|---|---|
| record_format |
ojs |
| spelling |
oai:scielo:S1678-919920170001003012016-09-22Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venomCostal-Oliveira,FernandaGuerra-Duarte,ClaraOliveira,Maira SouzaCastro,Karen Larissa Pereira deLopes-de-Sousa,LeticiaLara,AlineGomes,Enéas Ricardo de MoraisBonilla,CesarGuatimosim,SílviaMelo,Marília MartinsChávez-Olórtegui,Carlos Hadruroides lunatus venom Cardiorespiratory alterations Electrocardiography Immunofluorescence Calcium transient Abstract Background Hadruroides lunatus is the most abundant scorpion species in the Peruvian central coast, where most of the accidents involving humans are registered. In spite of its prevalence, there are only very few studies on H. lunatus envenomation. The aim of the present study was to analyze the cardiorespiratory alterations caused by H. lunatus envenomation in rodents. Methods Wistar rats injected with H. lunatus scorpion venom were submitted to electrocardiography. After euthanasia, rat lungs were collected and histopathologically analyzed. Mouse cardiomyocytes were used to perform immunofluorescence and calcium transient assays. Data were analyzed by ANOVA or Student’s t-test. The significance level was set at p< 0.05. Results It was observed that H. lunatus venom increased heart rate and caused arrhythmia, thereby impairing the heart functioning. Lungs of envenomed animals showed significant alterations, such as diffuse hemorrhage. In addition, immunofluorescence showed that H. lunatus venom was capable of binding to cardiomyocytes. Furthermore, mouse ventricular cardiomyocytes incubated with H. lunatus venom showed a significant decrease in calcium transient, confirming that H. lunatus venom exerts a toxic effect on heart. Conclusion Our results showed that H. lunatus venom is capable of inducing cardiorespiratory alterations, a typical systemic effect of scorpionism, stressing the importance of medical monitoring in envenomation cases.info:eu-repo/semantics/openAccessCentro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)Journal of Venomous Animals and Toxins including Tropical Diseases v.23 20172017-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100301en10.1186/s40409-016-0076-5 |
| institution |
SCIELO |
| collection |
OJS |
| country |
Brasil |
| countrycode |
BR |
| component |
Revista |
| access |
En linea |
| databasecode |
rev-scielo-br |
| tag |
revista |
| region |
America del Sur |
| libraryname |
SciELO |
| language |
English |
| format |
Digital |
| author |
Costal-Oliveira,Fernanda Guerra-Duarte,Clara Oliveira,Maira Souza Castro,Karen Larissa Pereira de Lopes-de-Sousa,Leticia Lara,Aline Gomes,Enéas Ricardo de Morais Bonilla,Cesar Guatimosim,Sílvia Melo,Marília Martins Chávez-Olórtegui,Carlos |
| spellingShingle |
Costal-Oliveira,Fernanda Guerra-Duarte,Clara Oliveira,Maira Souza Castro,Karen Larissa Pereira de Lopes-de-Sousa,Leticia Lara,Aline Gomes,Enéas Ricardo de Morais Bonilla,Cesar Guatimosim,Sílvia Melo,Marília Martins Chávez-Olórtegui,Carlos Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venom |
| author_facet |
Costal-Oliveira,Fernanda Guerra-Duarte,Clara Oliveira,Maira Souza Castro,Karen Larissa Pereira de Lopes-de-Sousa,Leticia Lara,Aline Gomes,Enéas Ricardo de Morais Bonilla,Cesar Guatimosim,Sílvia Melo,Marília Martins Chávez-Olórtegui,Carlos |
| author_sort |
Costal-Oliveira,Fernanda |
| title |
Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venom |
| title_short |
Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venom |
| title_full |
Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venom |
| title_fullStr |
Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venom |
| title_full_unstemmed |
Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venom |
| title_sort |
cardiorespiratory alterations in rodents experimentally envenomed with hadruroides lunatus scorpion venom |
| description |
Abstract Background Hadruroides lunatus is the most abundant scorpion species in the Peruvian central coast, where most of the accidents involving humans are registered. In spite of its prevalence, there are only very few studies on H. lunatus envenomation. The aim of the present study was to analyze the cardiorespiratory alterations caused by H. lunatus envenomation in rodents. Methods Wistar rats injected with H. lunatus scorpion venom were submitted to electrocardiography. After euthanasia, rat lungs were collected and histopathologically analyzed. Mouse cardiomyocytes were used to perform immunofluorescence and calcium transient assays. Data were analyzed by ANOVA or Student’s t-test. The significance level was set at p< 0.05. Results It was observed that H. lunatus venom increased heart rate and caused arrhythmia, thereby impairing the heart functioning. Lungs of envenomed animals showed significant alterations, such as diffuse hemorrhage. In addition, immunofluorescence showed that H. lunatus venom was capable of binding to cardiomyocytes. Furthermore, mouse ventricular cardiomyocytes incubated with H. lunatus venom showed a significant decrease in calcium transient, confirming that H. lunatus venom exerts a toxic effect on heart. Conclusion Our results showed that H. lunatus venom is capable of inducing cardiorespiratory alterations, a typical systemic effect of scorpionism, stressing the importance of medical monitoring in envenomation cases. |
| publisher |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
| publishDate |
2017 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100301 |
| work_keys_str_mv |
AT costaloliveirafernanda cardiorespiratoryalterationsinrodentsexperimentallyenvenomedwithhadruroideslunatusscorpionvenom AT guerraduarteclara cardiorespiratoryalterationsinrodentsexperimentallyenvenomedwithhadruroideslunatusscorpionvenom AT oliveiramairasouza cardiorespiratoryalterationsinrodentsexperimentallyenvenomedwithhadruroideslunatusscorpionvenom AT castrokarenlarissapereirade cardiorespiratoryalterationsinrodentsexperimentallyenvenomedwithhadruroideslunatusscorpionvenom AT lopesdesousaleticia cardiorespiratoryalterationsinrodentsexperimentallyenvenomedwithhadruroideslunatusscorpionvenom AT laraaline cardiorespiratoryalterationsinrodentsexperimentallyenvenomedwithhadruroideslunatusscorpionvenom AT gomeseneasricardodemorais cardiorespiratoryalterationsinrodentsexperimentallyenvenomedwithhadruroideslunatusscorpionvenom AT bonillacesar cardiorespiratoryalterationsinrodentsexperimentallyenvenomedwithhadruroideslunatusscorpionvenom AT guatimosimsilvia cardiorespiratoryalterationsinrodentsexperimentallyenvenomedwithhadruroideslunatusscorpionvenom AT melomariliamartins cardiorespiratoryalterationsinrodentsexperimentallyenvenomedwithhadruroideslunatusscorpionvenom AT chavezolorteguicarlos cardiorespiratoryalterationsinrodentsexperimentallyenvenomedwithhadruroideslunatusscorpionvenom |
| _version_ |
1756429225130196992 |