Association of Nd:YAG laser and calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion

Abstract Objective To evaluate the efficacy of Nd:YAG laser associated with calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion after erosive/abrasive challenges. Methodology Dentin specimens were exposed to 17% ethylene diamine tetra-acetic acid (EDTA) solution for 5 min and randomly allocated into five groups: G1, control (no treatment); G2, Nd:YAG laser (1 W, 10 Hz, 100 mJ, 85 J/cm2); G3, Laser + TeethmateTM Desensitizer; G4, Laser + Desensibilize Nano P; and G5, Laser+Nupro®. Specimens underwent a 5-day erosion-abrasion cycling. Hydraulic conductance was measured post-EDTA, post-treatment, and post-cycling. Post-treatment and post-cycling permeability (%Lp) was calculated based on post-EDTA measurements, considered 100%. Open dentin tubules (ODT) were calculated at the abovementioned experimental moments using scanning electron microscopy and ImageJ software (n=10). Data were analyzed using two-way repeated measures ANOVA and Tukey’s test (α=0.05). Results G1 presented the highest %Lp post-treatment of all groups (p<0.05), without significantly differences among them. At post-cycling, %Lp significantly decreased in G1, showed no significant differences from post-treatment in G3 and G4, and increased in G2 and G5, without significant differences from G1 (p>0.05). We found no significant differences in ODT among groups (p>0.05) post-EDTA. At post-treatment, treated groups did not differ from each other, but presented lower ODT than G1 (p<0.001). As for post-cycling, we verified no differences among groups (p>0.05), although ODT was significantly lower for all groups when compared to post-EDTA values (p<0.001). Conclusion All treatments effectively reduced dentin permeability and promoted tubule occlusion after application. Combining Nd YAG laser with calcium-phosphate pastes did not improve the laser effect. After erosive-abrasive challenges, treatments presented no differences when compared to the control.

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Main Authors: MAXIMIANO,Vinícius, MACHADO,Alana Cristina, LOPES,Raquel Marianna, RABELO,Fernanda Ellen Mercatelli, GARÓFALO,Stephanie Assimakopoulos, ZEZELL,Denise Maria, ARANHA,Ana Cecilia Corrêa, SCARAMUCCI,Taís
Format: Digital revista
Language:English
Published: Faculdade De Odontologia De Bauru - USP 2021
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572021000100412
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spelling oai:scielo:S1678-775720210001004122021-03-29Association of Nd:YAG laser and calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusionMAXIMIANO,ViníciusMACHADO,Alana CristinaLOPES,Raquel MariannaRABELO,Fernanda Ellen MercatelliGARÓFALO,Stephanie AssimakopoulosZEZELL,Denise MariaARANHA,Ana Cecilia CorrêaSCARAMUCCI,Taís Dentin hypersensitivity Laser Desensitizing agents Abstract Objective To evaluate the efficacy of Nd:YAG laser associated with calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion after erosive/abrasive challenges. Methodology Dentin specimens were exposed to 17% ethylene diamine tetra-acetic acid (EDTA) solution for 5 min and randomly allocated into five groups: G1, control (no treatment); G2, Nd:YAG laser (1 W, 10 Hz, 100 mJ, 85 J/cm2); G3, Laser + TeethmateTM Desensitizer; G4, Laser + Desensibilize Nano P; and G5, Laser+Nupro®. Specimens underwent a 5-day erosion-abrasion cycling. Hydraulic conductance was measured post-EDTA, post-treatment, and post-cycling. Post-treatment and post-cycling permeability (%Lp) was calculated based on post-EDTA measurements, considered 100%. Open dentin tubules (ODT) were calculated at the abovementioned experimental moments using scanning electron microscopy and ImageJ software (n=10). Data were analyzed using two-way repeated measures ANOVA and Tukey’s test (α=0.05). Results G1 presented the highest %Lp post-treatment of all groups (p<0.05), without significantly differences among them. At post-cycling, %Lp significantly decreased in G1, showed no significant differences from post-treatment in G3 and G4, and increased in G2 and G5, without significant differences from G1 (p>0.05). We found no significant differences in ODT among groups (p>0.05) post-EDTA. At post-treatment, treated groups did not differ from each other, but presented lower ODT than G1 (p<0.001). As for post-cycling, we verified no differences among groups (p>0.05), although ODT was significantly lower for all groups when compared to post-EDTA values (p<0.001). Conclusion All treatments effectively reduced dentin permeability and promoted tubule occlusion after application. Combining Nd YAG laser with calcium-phosphate pastes did not improve the laser effect. After erosive-abrasive challenges, treatments presented no differences when compared to the control.info:eu-repo/semantics/openAccessFaculdade De Odontologia De Bauru - USPJournal of Applied Oral Science v.29 20212021-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572021000100412en10.1590/1678-7757-2020-0736
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author MAXIMIANO,Vinícius
MACHADO,Alana Cristina
LOPES,Raquel Marianna
RABELO,Fernanda Ellen Mercatelli
GARÓFALO,Stephanie Assimakopoulos
ZEZELL,Denise Maria
ARANHA,Ana Cecilia Corrêa
SCARAMUCCI,Taís
spellingShingle MAXIMIANO,Vinícius
MACHADO,Alana Cristina
LOPES,Raquel Marianna
RABELO,Fernanda Ellen Mercatelli
GARÓFALO,Stephanie Assimakopoulos
ZEZELL,Denise Maria
ARANHA,Ana Cecilia Corrêa
SCARAMUCCI,Taís
Association of Nd:YAG laser and calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion
author_facet MAXIMIANO,Vinícius
MACHADO,Alana Cristina
LOPES,Raquel Marianna
RABELO,Fernanda Ellen Mercatelli
GARÓFALO,Stephanie Assimakopoulos
ZEZELL,Denise Maria
ARANHA,Ana Cecilia Corrêa
SCARAMUCCI,Taís
author_sort MAXIMIANO,Vinícius
title Association of Nd:YAG laser and calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion
title_short Association of Nd:YAG laser and calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion
title_full Association of Nd:YAG laser and calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion
title_fullStr Association of Nd:YAG laser and calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion
title_full_unstemmed Association of Nd:YAG laser and calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion
title_sort association of nd:yag laser and calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion
description Abstract Objective To evaluate the efficacy of Nd:YAG laser associated with calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion after erosive/abrasive challenges. Methodology Dentin specimens were exposed to 17% ethylene diamine tetra-acetic acid (EDTA) solution for 5 min and randomly allocated into five groups: G1, control (no treatment); G2, Nd:YAG laser (1 W, 10 Hz, 100 mJ, 85 J/cm2); G3, Laser + TeethmateTM Desensitizer; G4, Laser + Desensibilize Nano P; and G5, Laser+Nupro®. Specimens underwent a 5-day erosion-abrasion cycling. Hydraulic conductance was measured post-EDTA, post-treatment, and post-cycling. Post-treatment and post-cycling permeability (%Lp) was calculated based on post-EDTA measurements, considered 100%. Open dentin tubules (ODT) were calculated at the abovementioned experimental moments using scanning electron microscopy and ImageJ software (n=10). Data were analyzed using two-way repeated measures ANOVA and Tukey’s test (α=0.05). Results G1 presented the highest %Lp post-treatment of all groups (p<0.05), without significantly differences among them. At post-cycling, %Lp significantly decreased in G1, showed no significant differences from post-treatment in G3 and G4, and increased in G2 and G5, without significant differences from G1 (p>0.05). We found no significant differences in ODT among groups (p>0.05) post-EDTA. At post-treatment, treated groups did not differ from each other, but presented lower ODT than G1 (p<0.001). As for post-cycling, we verified no differences among groups (p>0.05), although ODT was significantly lower for all groups when compared to post-EDTA values (p<0.001). Conclusion All treatments effectively reduced dentin permeability and promoted tubule occlusion after application. Combining Nd YAG laser with calcium-phosphate pastes did not improve the laser effect. After erosive-abrasive challenges, treatments presented no differences when compared to the control.
publisher Faculdade De Odontologia De Bauru - USP
publishDate 2021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572021000100412
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