Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment

Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment

ABSTRACT Objective The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture. Material and Methods Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert. After 15 d, the CLSM analyzed the viable and dead bacteria with Live and Dead assay. The dentinal wall debris was collected by burs, and the colony forming units (CFU/mL) were counted. The penetrability of the paste inside dentinal tubules was tested using the B-rodamine dye. Results The calcium hydroxide paste showed better results with the use of ultrasonic agitation (p<0.05). Conclusion The ultrasonic agitation of CH paste increased its antimicrobial action and was responsible for intradentinal penetration with the fulfilment of the tubules.

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Main Authors: ARIAS,Marcela Paola Castro, MALIZA,Amanda Garcia Alves, MIDENA,Raquel Zanin, GRAEFF,Márcia Sirlene Zardin, DUARTE,Marco Antonio Húngaro, ANDRADE,Flaviana Bombarda de
Format: Digital revista
Language:English
Published: Faculdade De Odontologia De Bauru - USP 2016
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572016000600575
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spelling oai:scielo:S1678-775720160006005752017-01-03Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatmentARIAS,Marcela Paola CastroMALIZA,Amanda Garcia AlvesMIDENA,Raquel ZaninGRAEFF,Márcia Sirlene ZardinDUARTE,Marco Antonio HúngaroANDRADE,Flaviana Bombarda de Endodontics Calcium Hydroxide Enterococcus faecalis Ultrasound ABSTRACT Objective The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture. Material and Methods Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert. After 15 d, the CLSM analyzed the viable and dead bacteria with Live and Dead assay. The dentinal wall debris was collected by burs, and the colony forming units (CFU/mL) were counted. The penetrability of the paste inside dentinal tubules was tested using the B-rodamine dye. Results The calcium hydroxide paste showed better results with the use of ultrasonic agitation (p<0.05). Conclusion The ultrasonic agitation of CH paste increased its antimicrobial action and was responsible for intradentinal penetration with the fulfilment of the tubules.info:eu-repo/semantics/openAccessFaculdade De Odontologia De Bauru - USPJournal of Applied Oral Science v.24 n.6 20162016-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572016000600575en10.1590/1678-775720150553
institution SCIELO
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country Brasil
countrycode BR
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access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author ARIAS,Marcela Paola Castro
MALIZA,Amanda Garcia Alves
MIDENA,Raquel Zanin
GRAEFF,Márcia Sirlene Zardin
DUARTE,Marco Antonio Húngaro
ANDRADE,Flaviana Bombarda de
spellingShingle ARIAS,Marcela Paola Castro
MALIZA,Amanda Garcia Alves
MIDENA,Raquel Zanin
GRAEFF,Márcia Sirlene Zardin
DUARTE,Marco Antonio Húngaro
ANDRADE,Flaviana Bombarda de
Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
author_facet ARIAS,Marcela Paola Castro
MALIZA,Amanda Garcia Alves
MIDENA,Raquel Zanin
GRAEFF,Márcia Sirlene Zardin
DUARTE,Marco Antonio Húngaro
ANDRADE,Flaviana Bombarda de
author_sort ARIAS,Marcela Paola Castro
title Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
title_short Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
title_full Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
title_fullStr Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
title_full_unstemmed Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
title_sort effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
description ABSTRACT Objective The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture. Material and Methods Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert. After 15 d, the CLSM analyzed the viable and dead bacteria with Live and Dead assay. The dentinal wall debris was collected by burs, and the colony forming units (CFU/mL) were counted. The penetrability of the paste inside dentinal tubules was tested using the B-rodamine dye. Results The calcium hydroxide paste showed better results with the use of ultrasonic agitation (p<0.05). Conclusion The ultrasonic agitation of CH paste increased its antimicrobial action and was responsible for intradentinal penetration with the fulfilment of the tubules.
publisher Faculdade De Odontologia De Bauru - USP
publishDate 2016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572016000600575
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