A modified method by differential adhesion for enrichment of bladder cancer stem cells
ABSTRACT Purpose: In a previous study the vaccine was effective against bladder cancer in a mouse model. However, a small portion of tumors regrew because the vaccine could not eliminate bladder cancer stem cells (CSCs). In this study, we showed a modified method for the isolation of bladder CSCs using a combination of differential adhesion method and serum-free culture medium (SFM) method. Materials and Methods: Trypsin-resistant cells and trypsin-sensitive cells were isolated from MB49, EJ and 5637 cells by a combination of differential adhesion method and SFM method. The CSCs characterizations of trypsin-resistant cells were verified by the flow cytometry, the western blotting, the quantitative polymerase chain reaction, the resistance to chemotherapy assay, the transwell assay, and the tumor xenograft formation assay. Results: Trypsin-resistant cells were isolated and identified in CSCs characters, with high expression of CSCs markers, higher resistance to chemotherapy, greater migration in vitro, and stronger tumorigenicity in vivo. Conclusion: Trypsin-resistant cells displayed specific CSCs properties. Our study showed trypsin-resistant cells were isolated successfully with a modified method using a combination of differential adhesion method and SFM method.
Main Authors: | , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Urologia
2016
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382016000400817 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S1677-55382016000400817 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S1677-553820160004008172016-09-06A modified method by differential adhesion for enrichment of bladder cancer stem cellsZhu,Yong-tongPang,Shi-yuLuo,YangChen,WeiBao,Ji-mingTan,Wan-long Urinary Bladder Neoplasms Neoplastic Stem Cells Trypsin ABSTRACT Purpose: In a previous study the vaccine was effective against bladder cancer in a mouse model. However, a small portion of tumors regrew because the vaccine could not eliminate bladder cancer stem cells (CSCs). In this study, we showed a modified method for the isolation of bladder CSCs using a combination of differential adhesion method and serum-free culture medium (SFM) method. Materials and Methods: Trypsin-resistant cells and trypsin-sensitive cells were isolated from MB49, EJ and 5637 cells by a combination of differential adhesion method and SFM method. The CSCs characterizations of trypsin-resistant cells were verified by the flow cytometry, the western blotting, the quantitative polymerase chain reaction, the resistance to chemotherapy assay, the transwell assay, and the tumor xenograft formation assay. Results: Trypsin-resistant cells were isolated and identified in CSCs characters, with high expression of CSCs markers, higher resistance to chemotherapy, greater migration in vitro, and stronger tumorigenicity in vivo. Conclusion: Trypsin-resistant cells displayed specific CSCs properties. Our study showed trypsin-resistant cells were isolated successfully with a modified method using a combination of differential adhesion method and SFM method.info:eu-repo/semantics/openAccessSociedade Brasileira de UrologiaInternational braz j urol v.42 n.4 20162016-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382016000400817en10.1590/S1677-5538.IBJU.2015.0409 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Zhu,Yong-tong Pang,Shi-yu Luo,Yang Chen,Wei Bao,Ji-ming Tan,Wan-long |
spellingShingle |
Zhu,Yong-tong Pang,Shi-yu Luo,Yang Chen,Wei Bao,Ji-ming Tan,Wan-long A modified method by differential adhesion for enrichment of bladder cancer stem cells |
author_facet |
Zhu,Yong-tong Pang,Shi-yu Luo,Yang Chen,Wei Bao,Ji-ming Tan,Wan-long |
author_sort |
Zhu,Yong-tong |
title |
A modified method by differential adhesion for enrichment of bladder cancer stem cells |
title_short |
A modified method by differential adhesion for enrichment of bladder cancer stem cells |
title_full |
A modified method by differential adhesion for enrichment of bladder cancer stem cells |
title_fullStr |
A modified method by differential adhesion for enrichment of bladder cancer stem cells |
title_full_unstemmed |
A modified method by differential adhesion for enrichment of bladder cancer stem cells |
title_sort |
modified method by differential adhesion for enrichment of bladder cancer stem cells |
description |
ABSTRACT Purpose: In a previous study the vaccine was effective against bladder cancer in a mouse model. However, a small portion of tumors regrew because the vaccine could not eliminate bladder cancer stem cells (CSCs). In this study, we showed a modified method for the isolation of bladder CSCs using a combination of differential adhesion method and serum-free culture medium (SFM) method. Materials and Methods: Trypsin-resistant cells and trypsin-sensitive cells were isolated from MB49, EJ and 5637 cells by a combination of differential adhesion method and SFM method. The CSCs characterizations of trypsin-resistant cells were verified by the flow cytometry, the western blotting, the quantitative polymerase chain reaction, the resistance to chemotherapy assay, the transwell assay, and the tumor xenograft formation assay. Results: Trypsin-resistant cells were isolated and identified in CSCs characters, with high expression of CSCs markers, higher resistance to chemotherapy, greater migration in vitro, and stronger tumorigenicity in vivo. Conclusion: Trypsin-resistant cells displayed specific CSCs properties. Our study showed trypsin-resistant cells were isolated successfully with a modified method using a combination of differential adhesion method and SFM method. |
publisher |
Sociedade Brasileira de Urologia |
publishDate |
2016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382016000400817 |
work_keys_str_mv |
AT zhuyongtong amodifiedmethodbydifferentialadhesionforenrichmentofbladdercancerstemcells AT pangshiyu amodifiedmethodbydifferentialadhesionforenrichmentofbladdercancerstemcells AT luoyang amodifiedmethodbydifferentialadhesionforenrichmentofbladdercancerstemcells AT chenwei amodifiedmethodbydifferentialadhesionforenrichmentofbladdercancerstemcells AT baojiming amodifiedmethodbydifferentialadhesionforenrichmentofbladdercancerstemcells AT tanwanlong amodifiedmethodbydifferentialadhesionforenrichmentofbladdercancerstemcells AT zhuyongtong modifiedmethodbydifferentialadhesionforenrichmentofbladdercancerstemcells AT pangshiyu modifiedmethodbydifferentialadhesionforenrichmentofbladdercancerstemcells AT luoyang modifiedmethodbydifferentialadhesionforenrichmentofbladdercancerstemcells AT chenwei modifiedmethodbydifferentialadhesionforenrichmentofbladdercancerstemcells AT baojiming modifiedmethodbydifferentialadhesionforenrichmentofbladdercancerstemcells AT tanwanlong modifiedmethodbydifferentialadhesionforenrichmentofbladdercancerstemcells |
_version_ |
1756428206476361728 |