Molecular confirmation of twinning in the West Indian Manatee (Trichechus manatus)
Abstract: Few twinning events have been recorded in the West Indian manatee (Trichechus manatus, Sirenia: Trichechidae) and no previous published study has provided confirmation of this phenomenon based in molecular tools. Here we investigate a possible case of twinning in an endangered Brazilian population of T. manatus using molecular tools. We analyzed two male neonates found stranded in Ceará State, on the northeastern coast of Brazil. The DNA of both individuals was isolated, and 10 microsatellite loci were amplified and genotyped. Following the identification of the alleles, the probabilities of identity by descent (∆7 and ∆8) and relatedness (rxy) were calculated using estimators that evaluate inbreeding. The two individuals shared most of the alleles, with differences in the genotypes being identified in only two loci. All the estimators identified a level of relatedness compatible with that found between siblings (selfed or outbred), indicating they were dizygotic twins. This is the first confirmed case of fraternal twins in free-ranging West Indian manatees in South America. The recognition of this type of twinning provides elements to improve actions for the rehabilitation of stranded animals and their subsequent release to the environment.
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Instituto Virtual da Biodiversidade | BIOTA - FAPESP
2022
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oai:scielo:S1676-060320220001005012022-03-09Molecular confirmation of twinning in the West Indian Manatee (Trichechus manatus)Moreira,SáviaMeirelles,Ana Carolina O. deCarvalho,Vitor LuzRêgo,Péricles Sena doAraripe,Juliana Sirenians relatedness microsatellite stranding dizygotic twins Abstract: Few twinning events have been recorded in the West Indian manatee (Trichechus manatus, Sirenia: Trichechidae) and no previous published study has provided confirmation of this phenomenon based in molecular tools. Here we investigate a possible case of twinning in an endangered Brazilian population of T. manatus using molecular tools. We analyzed two male neonates found stranded in Ceará State, on the northeastern coast of Brazil. The DNA of both individuals was isolated, and 10 microsatellite loci were amplified and genotyped. Following the identification of the alleles, the probabilities of identity by descent (∆7 and ∆8) and relatedness (rxy) were calculated using estimators that evaluate inbreeding. The two individuals shared most of the alleles, with differences in the genotypes being identified in only two loci. All the estimators identified a level of relatedness compatible with that found between siblings (selfed or outbred), indicating they were dizygotic twins. This is the first confirmed case of fraternal twins in free-ranging West Indian manatees in South America. The recognition of this type of twinning provides elements to improve actions for the rehabilitation of stranded animals and their subsequent release to the environment.info:eu-repo/semantics/openAccessInstituto Virtual da Biodiversidade | BIOTA - FAPESPBiota Neotropica v.22 n.1 20222022-01-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032022000100501en10.1590/1676-0611-bn-2021-1241 |
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Moreira,Sávia Meirelles,Ana Carolina O. de Carvalho,Vitor Luz Rêgo,Péricles Sena do Araripe,Juliana |
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Moreira,Sávia Meirelles,Ana Carolina O. de Carvalho,Vitor Luz Rêgo,Péricles Sena do Araripe,Juliana Molecular confirmation of twinning in the West Indian Manatee (Trichechus manatus) |
author_facet |
Moreira,Sávia Meirelles,Ana Carolina O. de Carvalho,Vitor Luz Rêgo,Péricles Sena do Araripe,Juliana |
author_sort |
Moreira,Sávia |
title |
Molecular confirmation of twinning in the West Indian Manatee (Trichechus manatus) |
title_short |
Molecular confirmation of twinning in the West Indian Manatee (Trichechus manatus) |
title_full |
Molecular confirmation of twinning in the West Indian Manatee (Trichechus manatus) |
title_fullStr |
Molecular confirmation of twinning in the West Indian Manatee (Trichechus manatus) |
title_full_unstemmed |
Molecular confirmation of twinning in the West Indian Manatee (Trichechus manatus) |
title_sort |
molecular confirmation of twinning in the west indian manatee (trichechus manatus) |
description |
Abstract: Few twinning events have been recorded in the West Indian manatee (Trichechus manatus, Sirenia: Trichechidae) and no previous published study has provided confirmation of this phenomenon based in molecular tools. Here we investigate a possible case of twinning in an endangered Brazilian population of T. manatus using molecular tools. We analyzed two male neonates found stranded in Ceará State, on the northeastern coast of Brazil. The DNA of both individuals was isolated, and 10 microsatellite loci were amplified and genotyped. Following the identification of the alleles, the probabilities of identity by descent (∆7 and ∆8) and relatedness (rxy) were calculated using estimators that evaluate inbreeding. The two individuals shared most of the alleles, with differences in the genotypes being identified in only two loci. All the estimators identified a level of relatedness compatible with that found between siblings (selfed or outbred), indicating they were dizygotic twins. This is the first confirmed case of fraternal twins in free-ranging West Indian manatees in South America. The recognition of this type of twinning provides elements to improve actions for the rehabilitation of stranded animals and their subsequent release to the environment. |
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Instituto Virtual da Biodiversidade | BIOTA - FAPESP |
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2022 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032022000100501 |
work_keys_str_mv |
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