Phytochemical characterization and biological activities of Plectranthus barbatus Andrews
Abstract Plectranthus barbatus Andrews (Lamiaceae) is widely distributed in the world and has a range of popular therapeutic indications. This work aimed to evaluate the phytochemical characterization of two leaf extracts of P. barbatus, and their antimicrobial, antineoplastic and immunomodulatory potential. After collection, herborization and obtainment of the P. barbatus aqueous extract (PBA) and acetone:water 7:3 P. barbatus organic extract (PBO), the phytochemical characterization was performed by high-performance liquid chromatography (HPLC). The antimicrobial activity was performed to determine the minimum inhibitory concentration (MIC) against eight bacterial strains using the microdilution test and the fungus Trichophyton rubrum by disc diffusion assay and microdilution test. Cytotoxicity was assessed by MTT and trypan blue methods in normal peripheral blood mononuclear cells (PBMCs) at concentrations ranged between 0.1 to 100 µg.mL-1 and in neoplastic cell lines Toledo, K562, DU-145 and PANC-1 at 1, 10 and 100 µg.mL-1 . Immunomodulatory activity, was evaluated by sandwich ELISA of proinflammatory cytokines at BALB/c mice splenocytes cultures supernatant. Both extracts presented flavonoids, cinnamic derivatives, steroids and ellagic acid. PBO showed bacteriostatic activity against Acinetobacter baumannii (MIC = 250 µg.mL-1) clinical isolate and PBA fungistatic activity against Trichophyton rubrum (MIC = 800 µg.mL-1). The extracts did not exhibit toxicity to PBMCs and neoplastic cells (IC50 > 100 µg.mL-1). Additionally, PBO at 100 µg.mL-1 significantly inhibited IFN-γ and IL-17A cytokines (p = 0.03). Plectranthus barbatus is a potential candidate for therapeutic use due to its low toxicity in healthy human cells and exhibits biological activities of medical interest as bacteriostatic, fungistatic and immunomodulatory.
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Instituto Internacional de Ecologia
2022
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100122 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S1519-69842022000100122 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S1519-698420220001001222021-05-24Phytochemical characterization and biological activities of Plectranthus barbatus AndrewsCordeiro,M. F.Nunes,T. R. S.Bezerra,F. G.Damasco,P. K. M.Silva,W. A. V.Ferreira,M. R. A.Magalhães,O. M. C.Soares,L. A. L.Cavalcanti,I. M. F.Pitta,M. G. R.Rêgo,M. J. B. M. antimicrobial boldo cancer immunomodulation medicinal plant Abstract Plectranthus barbatus Andrews (Lamiaceae) is widely distributed in the world and has a range of popular therapeutic indications. This work aimed to evaluate the phytochemical characterization of two leaf extracts of P. barbatus, and their antimicrobial, antineoplastic and immunomodulatory potential. After collection, herborization and obtainment of the P. barbatus aqueous extract (PBA) and acetone:water 7:3 P. barbatus organic extract (PBO), the phytochemical characterization was performed by high-performance liquid chromatography (HPLC). The antimicrobial activity was performed to determine the minimum inhibitory concentration (MIC) against eight bacterial strains using the microdilution test and the fungus Trichophyton rubrum by disc diffusion assay and microdilution test. Cytotoxicity was assessed by MTT and trypan blue methods in normal peripheral blood mononuclear cells (PBMCs) at concentrations ranged between 0.1 to 100 µg.mL-1 and in neoplastic cell lines Toledo, K562, DU-145 and PANC-1 at 1, 10 and 100 µg.mL-1 . Immunomodulatory activity, was evaluated by sandwich ELISA of proinflammatory cytokines at BALB/c mice splenocytes cultures supernatant. Both extracts presented flavonoids, cinnamic derivatives, steroids and ellagic acid. PBO showed bacteriostatic activity against Acinetobacter baumannii (MIC = 250 µg.mL-1) clinical isolate and PBA fungistatic activity against Trichophyton rubrum (MIC = 800 µg.mL-1). The extracts did not exhibit toxicity to PBMCs and neoplastic cells (IC50 > 100 µg.mL-1). Additionally, PBO at 100 µg.mL-1 significantly inhibited IFN-γ and IL-17A cytokines (p = 0.03). Plectranthus barbatus is a potential candidate for therapeutic use due to its low toxicity in healthy human cells and exhibits biological activities of medical interest as bacteriostatic, fungistatic and immunomodulatory.info:eu-repo/semantics/openAccessInstituto Internacional de EcologiaBrazilian Journal of Biology v.82 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100122en10.1590/1519-6984.236297 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Cordeiro,M. F. Nunes,T. R. S. Bezerra,F. G. Damasco,P. K. M. Silva,W. A. V. Ferreira,M. R. A. Magalhães,O. M. C. Soares,L. A. L. Cavalcanti,I. M. F. Pitta,M. G. R. Rêgo,M. J. B. M. |
spellingShingle |
Cordeiro,M. F. Nunes,T. R. S. Bezerra,F. G. Damasco,P. K. M. Silva,W. A. V. Ferreira,M. R. A. Magalhães,O. M. C. Soares,L. A. L. Cavalcanti,I. M. F. Pitta,M. G. R. Rêgo,M. J. B. M. Phytochemical characterization and biological activities of Plectranthus barbatus Andrews |
author_facet |
Cordeiro,M. F. Nunes,T. R. S. Bezerra,F. G. Damasco,P. K. M. Silva,W. A. V. Ferreira,M. R. A. Magalhães,O. M. C. Soares,L. A. L. Cavalcanti,I. M. F. Pitta,M. G. R. Rêgo,M. J. B. M. |
author_sort |
Cordeiro,M. F. |
title |
Phytochemical characterization and biological activities of Plectranthus barbatus Andrews |
title_short |
Phytochemical characterization and biological activities of Plectranthus barbatus Andrews |
title_full |
Phytochemical characterization and biological activities of Plectranthus barbatus Andrews |
title_fullStr |
Phytochemical characterization and biological activities of Plectranthus barbatus Andrews |
title_full_unstemmed |
Phytochemical characterization and biological activities of Plectranthus barbatus Andrews |
title_sort |
phytochemical characterization and biological activities of plectranthus barbatus andrews |
description |
Abstract Plectranthus barbatus Andrews (Lamiaceae) is widely distributed in the world and has a range of popular therapeutic indications. This work aimed to evaluate the phytochemical characterization of two leaf extracts of P. barbatus, and their antimicrobial, antineoplastic and immunomodulatory potential. After collection, herborization and obtainment of the P. barbatus aqueous extract (PBA) and acetone:water 7:3 P. barbatus organic extract (PBO), the phytochemical characterization was performed by high-performance liquid chromatography (HPLC). The antimicrobial activity was performed to determine the minimum inhibitory concentration (MIC) against eight bacterial strains using the microdilution test and the fungus Trichophyton rubrum by disc diffusion assay and microdilution test. Cytotoxicity was assessed by MTT and trypan blue methods in normal peripheral blood mononuclear cells (PBMCs) at concentrations ranged between 0.1 to 100 µg.mL-1 and in neoplastic cell lines Toledo, K562, DU-145 and PANC-1 at 1, 10 and 100 µg.mL-1 . Immunomodulatory activity, was evaluated by sandwich ELISA of proinflammatory cytokines at BALB/c mice splenocytes cultures supernatant. Both extracts presented flavonoids, cinnamic derivatives, steroids and ellagic acid. PBO showed bacteriostatic activity against Acinetobacter baumannii (MIC = 250 µg.mL-1) clinical isolate and PBA fungistatic activity against Trichophyton rubrum (MIC = 800 µg.mL-1). The extracts did not exhibit toxicity to PBMCs and neoplastic cells (IC50 > 100 µg.mL-1). Additionally, PBO at 100 µg.mL-1 significantly inhibited IFN-γ and IL-17A cytokines (p = 0.03). Plectranthus barbatus is a potential candidate for therapeutic use due to its low toxicity in healthy human cells and exhibits biological activities of medical interest as bacteriostatic, fungistatic and immunomodulatory. |
publisher |
Instituto Internacional de Ecologia |
publishDate |
2022 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100122 |
work_keys_str_mv |
AT cordeiromf phytochemicalcharacterizationandbiologicalactivitiesofplectranthusbarbatusandrews AT nunestrs phytochemicalcharacterizationandbiologicalactivitiesofplectranthusbarbatusandrews AT bezerrafg phytochemicalcharacterizationandbiologicalactivitiesofplectranthusbarbatusandrews AT damascopkm phytochemicalcharacterizationandbiologicalactivitiesofplectranthusbarbatusandrews AT silvawav phytochemicalcharacterizationandbiologicalactivitiesofplectranthusbarbatusandrews AT ferreiramra phytochemicalcharacterizationandbiologicalactivitiesofplectranthusbarbatusandrews AT magalhaesomc phytochemicalcharacterizationandbiologicalactivitiesofplectranthusbarbatusandrews AT soareslal phytochemicalcharacterizationandbiologicalactivitiesofplectranthusbarbatusandrews AT cavalcantiimf phytochemicalcharacterizationandbiologicalactivitiesofplectranthusbarbatusandrews AT pittamgr phytochemicalcharacterizationandbiologicalactivitiesofplectranthusbarbatusandrews AT regomjbm phytochemicalcharacterizationandbiologicalactivitiesofplectranthusbarbatusandrews |
_version_ |
1756426815968116736 |