Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus
The present study evaluated the assay to quantify the respiratory burst activity of blood leukocytes of pacu as an indicator of the innate immune system, using the reduction of nitroblue tetrazolium (NBT) to formazan as a measure of the production of reactive oxygen species (ROS). In order to assess the accuracy of the assay, fish were challenged by Aeromonas hydrophila and sampled one week after challenge. The A. hydrophila infection increased the leukocyte respiratory burst activity. The protocol showed a reliable and easy assay, appropriate to determine the respiratory burst activity of blood leukocytes of pacu, a neotropical fish, in the present experimental conditions.
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Instituto Internacional de Ecologia
2013
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oai:scielo:S1519-698420130002004252015-10-27Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicusBiller-Takahashi,JDTakahashi,LSSaita,MVGimbo,RYUrbinati,EC immune methodology fish immunology cellular immunity The present study evaluated the assay to quantify the respiratory burst activity of blood leukocytes of pacu as an indicator of the innate immune system, using the reduction of nitroblue tetrazolium (NBT) to formazan as a measure of the production of reactive oxygen species (ROS). In order to assess the accuracy of the assay, fish were challenged by Aeromonas hydrophila and sampled one week after challenge. The A. hydrophila infection increased the leukocyte respiratory burst activity. The protocol showed a reliable and easy assay, appropriate to determine the respiratory burst activity of blood leukocytes of pacu, a neotropical fish, in the present experimental conditions.info:eu-repo/semantics/openAccessInstituto Internacional de EcologiaBrazilian Journal of Biology v.73 n.2 20132013-05-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842013000200425en10.1590/S1519-69842013000200026 |
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Biller-Takahashi,JD Takahashi,LS Saita,MV Gimbo,RY Urbinati,EC |
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Biller-Takahashi,JD Takahashi,LS Saita,MV Gimbo,RY Urbinati,EC Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus |
| author_facet |
Biller-Takahashi,JD Takahashi,LS Saita,MV Gimbo,RY Urbinati,EC |
| author_sort |
Biller-Takahashi,JD |
| title |
Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus |
| title_short |
Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus |
| title_full |
Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus |
| title_fullStr |
Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus |
| title_full_unstemmed |
Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus |
| title_sort |
leukocytes respiratory burst activity as indicator of innate immunity of pacu piaractus mesopotamicus |
| description |
The present study evaluated the assay to quantify the respiratory burst activity of blood leukocytes of pacu as an indicator of the innate immune system, using the reduction of nitroblue tetrazolium (NBT) to formazan as a measure of the production of reactive oxygen species (ROS). In order to assess the accuracy of the assay, fish were challenged by Aeromonas hydrophila and sampled one week after challenge. The A. hydrophila infection increased the leukocyte respiratory burst activity. The protocol showed a reliable and easy assay, appropriate to determine the respiratory burst activity of blood leukocytes of pacu, a neotropical fish, in the present experimental conditions. |
| publisher |
Instituto Internacional de Ecologia |
| publishDate |
2013 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842013000200425 |
| work_keys_str_mv |
AT billertakahashijd leukocytesrespiratoryburstactivityasindicatorofinnateimmunityofpacupiaractusmesopotamicus AT takahashils leukocytesrespiratoryburstactivityasindicatorofinnateimmunityofpacupiaractusmesopotamicus AT saitamv leukocytesrespiratoryburstactivityasindicatorofinnateimmunityofpacupiaractusmesopotamicus AT gimbory leukocytesrespiratoryburstactivityasindicatorofinnateimmunityofpacupiaractusmesopotamicus AT urbinatiec leukocytesrespiratoryburstactivityasindicatorofinnateimmunityofpacupiaractusmesopotamicus |
| _version_ |
1756426518212378624 |