BIOSENSOR DEVELOPMENT IN SPORTS DOPING WITH DEXAMETHASONE

ABSTRACT Introduction: Dexamethasone is a type of drug that is considered a steroid. It belongs to a class of drugs known as corticosteroids. Objective: Develop an electrochemical sensor of dexamethasone in a pharmaceutical sample using electrodes modified with nanostructures of MnO2 and reduced graphene oxide (MnO2/rGO). The glassy carbon electrodes (GCE) used to make the GO nanostructures were first modified using a modified Hummers technique before electrochemically reduced. Methods: MnO2 nanomaterials were electrochemically deposited on rGO/GCE. SEM structural investigation indicated vertical tetragonal crystal development of -MnO2 nanostructures in sprayed rGO nanostructures. Results: Because of the high composite surface area, multiple exposed active sites, and the synergistic effect of MnO2 and rGO, the electrocatalytic reaction to dexamethasone of MnO2/rGO/CPE was shown to be broad, selective, stable, and sensitive in electrochemical tests using amperometry. It was established that the linear range, sensitivities, and detection limit of the sensor are 0 to 260 µM, 4.6153µA/µM and 0.005 µM, respectively. The MnO2/rGO/CPE was tested for accuracy and applicability in determining dexamethasone in pharmacological and human urine samples. Conclusion: The results revealed that the sensor could prepare acceptable recovery (96.34%) and RSD (3.58%), suggesting that it could be used as a reliable dexamethasone sensor in clinical samples. Level of evidence II; Therapeutic studies - Investigation of treatment outcomes.

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Bibliographic Details
Main Authors: Purnama,Sapta Kunta, Doewes,Rumi Iqbal, Elumalai,Gunathevan, Azmi,Siti Hartini, Nuryadin,Islahuzzaman, Manshuralhudlori
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina do Exercício e do Esporte 2023
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-86922023000100289
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