Inducible cellulase production from an organic solvent tolerant Bacillus sp. SV1 and evolutionary divergence of endoglucanase in different species of the genus Bacillus
Abstract Bacteria are important sources of cellulases with various industrial and biotechnological applications. In view of this, a non-hemolytic bacterial strain, tolerant to various environmental pollutants (heavy metals and organic solvents), showing high cellulolytic index (7.89) was isolated from cattle shed soil and identified as Bacillus sp. SV1 (99.27% pairwise similarity with Bacillus korlensis). Extracellular cellulases showed the presence of endoglucanase, total cellulase and β-glucosidase activities. Cellulase production was induced in presence of cellulose (3.3 times CMCase, 2.9 times FPase and 2.1 times β-glucosidase), and enhanced (115.1% CMCase) by low-cost corn steep solids. An in silico investigation of endoglucanase (EC 3.2.1.4) protein sequences of three Bacillus spp. as query, revealed their similarities with members of nine bacterial phyla and to Eukaryota (represented by Arthropoda and Nematoda), and also highlighted of a convergent and divergent evolution from other enzymes of different substrate [(1,3)-linked beta-d-glucans, xylan and chitosan] specificities. Characteristic conserved signature indels were observed among members of Actinobacteria (7 aa insert) and Firmicutes (9 aa insert) that served as a potential tool in support of their relatedness in phylogenetic trees.
Main Authors: | Thomas,Lebin, Ram,Hari, Singh,Ved Pal |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Microbiologia
2018
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200429 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Chitinase and cellulase activity from Bacillus thuringiensis strains.
by: DUMAS, V. F., et al.
Published: (2011-03-30) -
Cellulase production by thermophilic Bacillus sp: SMIA-2 and its detergent compatibility
by: Ladeira,Silvania A, et al.
Published: (2015) -
Production, purification and characterization of an ionic liquid tolerant cellulase from Bacillus sp. isolated from rice paddy field soil
by: Sriariyanun,Malinee, et al.
Published: (2016) -
Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost
by: Chang,Chen-Chin, et al.
Published: (2009) -
Susceptibilidade de larvas de Spodoptera frugiperda de varias idades, a Bacillus thurigiensis sv. tolworthi.
by: MARTINS, M. F., et al.
Published: (2003-09-25)