Zika detection: comparison of methodologies
ABSTRACT Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan® RT-qPCR assay. We used a SYBR® Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan® RT-qPCR assay, 100% (Kappa = 0.670) were also found to be negative by SYBR® Green RT-qPCR based on Tm comparison; however, 14% (Kappa = 0.035) were found to be positive by conventional PCR followed by agarose gel electrophoresis. The differences between the ZIKV strains circulating worldwide and the low viremia period can compromise diagnostic accuracy and thereby the accuracy of outbreak data. Therefore, improved assays are required to improve the diagnosis and surveillance of arbovirus.
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Sociedade Brasileira de Microbiologia
2018
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oai:scielo:S1517-838220180001001442018-02-20Zika detection: comparison of methodologiesColombo,Tatiana EliasTerzian,Ana Carolina BernardesAraújo Júnior,João PessoaParreira,RicardoCabrera,Eliana Márcia SotelloSantos,Izalco Nuremberg Penha dosReis,Andréia Francesli NegriCosta,Fabiana RodriguesCruz,Lilian Elisa Arão AntônioRombola,Patrícia LopesNogueira,Maurício Lacerda Zika virus Detection Sensitivity Specificity ABSTRACT Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan® RT-qPCR assay. We used a SYBR® Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan® RT-qPCR assay, 100% (Kappa = 0.670) were also found to be negative by SYBR® Green RT-qPCR based on Tm comparison; however, 14% (Kappa = 0.035) were found to be positive by conventional PCR followed by agarose gel electrophoresis. The differences between the ZIKV strains circulating worldwide and the low viremia period can compromise diagnostic accuracy and thereby the accuracy of outbreak data. Therefore, improved assays are required to improve the diagnosis and surveillance of arbovirus.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology v.49 n.1 20182018-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100144en10.1016/j.bjm.2017.04.011 |
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Colombo,Tatiana Elias Terzian,Ana Carolina Bernardes Araújo Júnior,João Pessoa Parreira,Ricardo Cabrera,Eliana Márcia Sotello Santos,Izalco Nuremberg Penha dos Reis,Andréia Francesli Negri Costa,Fabiana Rodrigues Cruz,Lilian Elisa Arão Antônio Rombola,Patrícia Lopes Nogueira,Maurício Lacerda |
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Colombo,Tatiana Elias Terzian,Ana Carolina Bernardes Araújo Júnior,João Pessoa Parreira,Ricardo Cabrera,Eliana Márcia Sotello Santos,Izalco Nuremberg Penha dos Reis,Andréia Francesli Negri Costa,Fabiana Rodrigues Cruz,Lilian Elisa Arão Antônio Rombola,Patrícia Lopes Nogueira,Maurício Lacerda Zika detection: comparison of methodologies |
author_facet |
Colombo,Tatiana Elias Terzian,Ana Carolina Bernardes Araújo Júnior,João Pessoa Parreira,Ricardo Cabrera,Eliana Márcia Sotello Santos,Izalco Nuremberg Penha dos Reis,Andréia Francesli Negri Costa,Fabiana Rodrigues Cruz,Lilian Elisa Arão Antônio Rombola,Patrícia Lopes Nogueira,Maurício Lacerda |
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Colombo,Tatiana Elias |
title |
Zika detection: comparison of methodologies |
title_short |
Zika detection: comparison of methodologies |
title_full |
Zika detection: comparison of methodologies |
title_fullStr |
Zika detection: comparison of methodologies |
title_full_unstemmed |
Zika detection: comparison of methodologies |
title_sort |
zika detection: comparison of methodologies |
description |
ABSTRACT Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan® RT-qPCR assay. We used a SYBR® Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan® RT-qPCR assay, 100% (Kappa = 0.670) were also found to be negative by SYBR® Green RT-qPCR based on Tm comparison; however, 14% (Kappa = 0.035) were found to be positive by conventional PCR followed by agarose gel electrophoresis. The differences between the ZIKV strains circulating worldwide and the low viremia period can compromise diagnostic accuracy and thereby the accuracy of outbreak data. Therefore, improved assays are required to improve the diagnosis and surveillance of arbovirus. |
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Sociedade Brasileira de Microbiologia |
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2018 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100144 |
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