A novel expression vector for the secretion of abaecin in Bacillus subtilis
ABSTRACT This study aimed to describe a Bacillus subtilis expression system based on genetically modified B. subtilis. Abaecin, an antimicrobial peptide obtained from Apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. For the exogenous expression, the abaecin gene was fused with a tobacco etch virus protease cleavage site, a promoter Pglv, and a mature beta-glucanase signal peptide. Also, a B. subtilis expression system was constructed. The recombinant abaecin gene was expressed and purified as a recombinant protein in the culture supernatant. The purified abaecin did not inhibit the growth of Escherichia coli strain K88. Cecropin A and hymenoptaecin exhibited potent bactericidal activities at concentrations of 1 and 1.5 µM. Combinatorial assays revealed that cecropin A and hymenoptaecin had sublethal concentrations of 0.3 and 0.5 µM. This potentiating functional interaction represents a promising therapeutic strategy. It provides an opportunity to address the rising threat of multidrug-resistant pathogens that are recalcitrant to conventional antibiotics.
Main Authors: | , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Microbiologia
2017
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400809 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S1517-83822017000400809 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S1517-838220170004008092017-10-31A novel expression vector for the secretion of abaecin in Bacillus subtilisLi,LiMu,LanWang,XiaojuanYu,JingfengHu,RuipingLi,Zhen Abaecin Antimicrobial peptides Bacillus subtilis Expression ABSTRACT This study aimed to describe a Bacillus subtilis expression system based on genetically modified B. subtilis. Abaecin, an antimicrobial peptide obtained from Apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. For the exogenous expression, the abaecin gene was fused with a tobacco etch virus protease cleavage site, a promoter Pglv, and a mature beta-glucanase signal peptide. Also, a B. subtilis expression system was constructed. The recombinant abaecin gene was expressed and purified as a recombinant protein in the culture supernatant. The purified abaecin did not inhibit the growth of Escherichia coli strain K88. Cecropin A and hymenoptaecin exhibited potent bactericidal activities at concentrations of 1 and 1.5 µM. Combinatorial assays revealed that cecropin A and hymenoptaecin had sublethal concentrations of 0.3 and 0.5 µM. This potentiating functional interaction represents a promising therapeutic strategy. It provides an opportunity to address the rising threat of multidrug-resistant pathogens that are recalcitrant to conventional antibiotics.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology v.48 n.4 20172017-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400809en10.1016/j.bjm.2017.01.009 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Li,Li Mu,Lan Wang,Xiaojuan Yu,Jingfeng Hu,Ruiping Li,Zhen |
spellingShingle |
Li,Li Mu,Lan Wang,Xiaojuan Yu,Jingfeng Hu,Ruiping Li,Zhen A novel expression vector for the secretion of abaecin in Bacillus subtilis |
author_facet |
Li,Li Mu,Lan Wang,Xiaojuan Yu,Jingfeng Hu,Ruiping Li,Zhen |
author_sort |
Li,Li |
title |
A novel expression vector for the secretion of abaecin in Bacillus subtilis |
title_short |
A novel expression vector for the secretion of abaecin in Bacillus subtilis |
title_full |
A novel expression vector for the secretion of abaecin in Bacillus subtilis |
title_fullStr |
A novel expression vector for the secretion of abaecin in Bacillus subtilis |
title_full_unstemmed |
A novel expression vector for the secretion of abaecin in Bacillus subtilis |
title_sort |
novel expression vector for the secretion of abaecin in bacillus subtilis |
description |
ABSTRACT This study aimed to describe a Bacillus subtilis expression system based on genetically modified B. subtilis. Abaecin, an antimicrobial peptide obtained from Apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. For the exogenous expression, the abaecin gene was fused with a tobacco etch virus protease cleavage site, a promoter Pglv, and a mature beta-glucanase signal peptide. Also, a B. subtilis expression system was constructed. The recombinant abaecin gene was expressed and purified as a recombinant protein in the culture supernatant. The purified abaecin did not inhibit the growth of Escherichia coli strain K88. Cecropin A and hymenoptaecin exhibited potent bactericidal activities at concentrations of 1 and 1.5 µM. Combinatorial assays revealed that cecropin A and hymenoptaecin had sublethal concentrations of 0.3 and 0.5 µM. This potentiating functional interaction represents a promising therapeutic strategy. It provides an opportunity to address the rising threat of multidrug-resistant pathogens that are recalcitrant to conventional antibiotics. |
publisher |
Sociedade Brasileira de Microbiologia |
publishDate |
2017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400809 |
work_keys_str_mv |
AT lili anovelexpressionvectorforthesecretionofabaecininbacillussubtilis AT mulan anovelexpressionvectorforthesecretionofabaecininbacillussubtilis AT wangxiaojuan anovelexpressionvectorforthesecretionofabaecininbacillussubtilis AT yujingfeng anovelexpressionvectorforthesecretionofabaecininbacillussubtilis AT huruiping anovelexpressionvectorforthesecretionofabaecininbacillussubtilis AT lizhen anovelexpressionvectorforthesecretionofabaecininbacillussubtilis AT lili novelexpressionvectorforthesecretionofabaecininbacillussubtilis AT mulan novelexpressionvectorforthesecretionofabaecininbacillussubtilis AT wangxiaojuan novelexpressionvectorforthesecretionofabaecininbacillussubtilis AT yujingfeng novelexpressionvectorforthesecretionofabaecininbacillussubtilis AT huruiping novelexpressionvectorforthesecretionofabaecininbacillussubtilis AT lizhen novelexpressionvectorforthesecretionofabaecininbacillussubtilis |
_version_ |
1756425128760049664 |