The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China

The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China

Abstract In this study, determination of heavy metal parameters and microbiological characterization of marine sediments obtained from two heavily polluted sites and one low-grade contaminated reference station at Jiaozhou Bay in China were carried out. The microbial communities found in the sampled marine sediments were studied using PCR-DGGE (denaturing gradient gel electrophoresis) fingerprinting profiles in combination with multivariate analysis. Clustering analysis of DGGE and matrix of heavy metals displayed similar occurrence patterns. On this basis, 17 samples were classified into two clusters depending on the presence or absence of the high level contamination. Moreover, the cluster of highly contaminated samples was further classified into two sub-groups based on the stations of their origin. These results showed that the composition of the bacterial community is strongly influenced by heavy metal variables present in the sediments found in the Jiaozhou Bay. This study also suggested that metagenomic techniques such as PCR-DGGE fingerprinting in combination with multivariate analysis is an efficient method to examine the effect of metal contamination on the bacterial community structure.

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Main Authors: Yao,Xie-feng, Zhang,Jiu-ming, Tian,Li, Guo,Jian-hua
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Microbiologia 2017
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100071
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spelling oai:scielo:S1517-838220170001000712017-01-24The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, ChinaYao,Xie-fengZhang,Jiu-mingTian,LiGuo,Jian-hua Heavy metal contamination PCR-DGGE fingerprinting Multivariate analysis Bacterial community structure Abstract In this study, determination of heavy metal parameters and microbiological characterization of marine sediments obtained from two heavily polluted sites and one low-grade contaminated reference station at Jiaozhou Bay in China were carried out. The microbial communities found in the sampled marine sediments were studied using PCR-DGGE (denaturing gradient gel electrophoresis) fingerprinting profiles in combination with multivariate analysis. Clustering analysis of DGGE and matrix of heavy metals displayed similar occurrence patterns. On this basis, 17 samples were classified into two clusters depending on the presence or absence of the high level contamination. Moreover, the cluster of highly contaminated samples was further classified into two sub-groups based on the stations of their origin. These results showed that the composition of the bacterial community is strongly influenced by heavy metal variables present in the sediments found in the Jiaozhou Bay. This study also suggested that metagenomic techniques such as PCR-DGGE fingerprinting in combination with multivariate analysis is an efficient method to examine the effect of metal contamination on the bacterial community structure.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology v.48 n.1 20172017-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100071en10.1016/j.bjm.2016.09.007
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Yao,Xie-feng
Zhang,Jiu-ming
Tian,Li
Guo,Jian-hua
spellingShingle Yao,Xie-feng
Zhang,Jiu-ming
Tian,Li
Guo,Jian-hua
The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China
author_facet Yao,Xie-feng
Zhang,Jiu-ming
Tian,Li
Guo,Jian-hua
author_sort Yao,Xie-feng
title The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China
title_short The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China
title_full The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China
title_fullStr The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China
title_full_unstemmed The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China
title_sort effect of heavy metal contamination on the bacterial community structure at jiaozhou bay, china
description Abstract In this study, determination of heavy metal parameters and microbiological characterization of marine sediments obtained from two heavily polluted sites and one low-grade contaminated reference station at Jiaozhou Bay in China were carried out. The microbial communities found in the sampled marine sediments were studied using PCR-DGGE (denaturing gradient gel electrophoresis) fingerprinting profiles in combination with multivariate analysis. Clustering analysis of DGGE and matrix of heavy metals displayed similar occurrence patterns. On this basis, 17 samples were classified into two clusters depending on the presence or absence of the high level contamination. Moreover, the cluster of highly contaminated samples was further classified into two sub-groups based on the stations of their origin. These results showed that the composition of the bacterial community is strongly influenced by heavy metal variables present in the sediments found in the Jiaozhou Bay. This study also suggested that metagenomic techniques such as PCR-DGGE fingerprinting in combination with multivariate analysis is an efficient method to examine the effect of metal contamination on the bacterial community structure.
publisher Sociedade Brasileira de Microbiologia
publishDate 2017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100071
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