Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi
Abstract This study aimed to evaluate the in vitro antifungal activity of terpinen-4-ol, tyrosol, and β-lapachone against strains of Coccidioides posadasii in filamentous phase (n = 22) and Histoplasma capsulatum in both filamentous (n = 40) and yeast phases (n = 13), using the broth dilution methods as described by the Clinical and Laboratory Standards Institute, to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of these compounds. The mechanisms of action of these compounds were also investigated by analyzing their effect on cell membrane permeability and ergosterol synthesis. The MIC and MFCf these compounds against C. posadasii, mycelial H. capsulatum, and yeast-like H. capsulatum, were in the following ranges: 350-5720 µg/mL, 20-2860 µg/mL, and 40-1420 µg/mL, respectively for terpinen-4-ol; 250-4000 µg/mL, 30-2000 µg/mL, and 10-1000 µg/mL, respectively, for tyrosol; and 0.48-7.8 µg/mL, 0.25-16 µg/mL, and 0.125-4 µg/mL, respectively for β-lapachone. These compounds showed a decrease in MIC when the samples were subjected to osmotic stress, suggesting that the compounds acted on the fungal membrane. All the compounds were able to reduce the ergosterol content of the fungal strains. Finally, tyrosol was able to cause a leakage of intracellular molecules.
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Sociedade Brasileira de Microbiologia
2016
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oai:scielo:S1517-838220160004009172016-11-21Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungiBrilhante,Raimunda Sâmia NogueiraCaetano,Érica PachecoLima,Rita Amanda Chaves deMarques,Francisca Jakelyne de FariasCastelo-Branco,Débora de Souza Collares MaiaMelo,Charlline Vládia Silva deGuedes,Glaucia Morgana de MeloOliveira,Jonathas Sales deCamargo,Zoilo Pires deMoreira,José Luciano BezerraMonteiro,André JallesBandeira,Tereza de Jesus Pinheiro GomesCordeiro,Rossana de AguiarRocha,Marcos Fábio GadelhaSidrim,José Júlio Costa Dimorphic fungi Terpinen-4-ol Tyrosol β-Lapachone Inhibitory effect Abstract This study aimed to evaluate the in vitro antifungal activity of terpinen-4-ol, tyrosol, and β-lapachone against strains of Coccidioides posadasii in filamentous phase (n = 22) and Histoplasma capsulatum in both filamentous (n = 40) and yeast phases (n = 13), using the broth dilution methods as described by the Clinical and Laboratory Standards Institute, to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of these compounds. The mechanisms of action of these compounds were also investigated by analyzing their effect on cell membrane permeability and ergosterol synthesis. The MIC and MFCf these compounds against C. posadasii, mycelial H. capsulatum, and yeast-like H. capsulatum, were in the following ranges: 350-5720 µg/mL, 20-2860 µg/mL, and 40-1420 µg/mL, respectively for terpinen-4-ol; 250-4000 µg/mL, 30-2000 µg/mL, and 10-1000 µg/mL, respectively, for tyrosol; and 0.48-7.8 µg/mL, 0.25-16 µg/mL, and 0.125-4 µg/mL, respectively for β-lapachone. These compounds showed a decrease in MIC when the samples were subjected to osmotic stress, suggesting that the compounds acted on the fungal membrane. All the compounds were able to reduce the ergosterol content of the fungal strains. Finally, tyrosol was able to cause a leakage of intracellular molecules.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology v.47 n.4 20162016-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400917en10.1016/j.bjm.2016.07.015 |
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Brilhante,Raimunda Sâmia Nogueira Caetano,Érica Pacheco Lima,Rita Amanda Chaves de Marques,Francisca Jakelyne de Farias Castelo-Branco,Débora de Souza Collares Maia Melo,Charlline Vládia Silva de Guedes,Glaucia Morgana de Melo Oliveira,Jonathas Sales de Camargo,Zoilo Pires de Moreira,José Luciano Bezerra Monteiro,André Jalles Bandeira,Tereza de Jesus Pinheiro Gomes Cordeiro,Rossana de Aguiar Rocha,Marcos Fábio Gadelha Sidrim,José Júlio Costa |
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Brilhante,Raimunda Sâmia Nogueira Caetano,Érica Pacheco Lima,Rita Amanda Chaves de Marques,Francisca Jakelyne de Farias Castelo-Branco,Débora de Souza Collares Maia Melo,Charlline Vládia Silva de Guedes,Glaucia Morgana de Melo Oliveira,Jonathas Sales de Camargo,Zoilo Pires de Moreira,José Luciano Bezerra Monteiro,André Jalles Bandeira,Tereza de Jesus Pinheiro Gomes Cordeiro,Rossana de Aguiar Rocha,Marcos Fábio Gadelha Sidrim,José Júlio Costa Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi |
author_facet |
Brilhante,Raimunda Sâmia Nogueira Caetano,Érica Pacheco Lima,Rita Amanda Chaves de Marques,Francisca Jakelyne de Farias Castelo-Branco,Débora de Souza Collares Maia Melo,Charlline Vládia Silva de Guedes,Glaucia Morgana de Melo Oliveira,Jonathas Sales de Camargo,Zoilo Pires de Moreira,José Luciano Bezerra Monteiro,André Jalles Bandeira,Tereza de Jesus Pinheiro Gomes Cordeiro,Rossana de Aguiar Rocha,Marcos Fábio Gadelha Sidrim,José Júlio Costa |
author_sort |
Brilhante,Raimunda Sâmia Nogueira |
title |
Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi |
title_short |
Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi |
title_full |
Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi |
title_fullStr |
Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi |
title_full_unstemmed |
Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi |
title_sort |
terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi |
description |
Abstract This study aimed to evaluate the in vitro antifungal activity of terpinen-4-ol, tyrosol, and β-lapachone against strains of Coccidioides posadasii in filamentous phase (n = 22) and Histoplasma capsulatum in both filamentous (n = 40) and yeast phases (n = 13), using the broth dilution methods as described by the Clinical and Laboratory Standards Institute, to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of these compounds. The mechanisms of action of these compounds were also investigated by analyzing their effect on cell membrane permeability and ergosterol synthesis. The MIC and MFCf these compounds against C. posadasii, mycelial H. capsulatum, and yeast-like H. capsulatum, were in the following ranges: 350-5720 µg/mL, 20-2860 µg/mL, and 40-1420 µg/mL, respectively for terpinen-4-ol; 250-4000 µg/mL, 30-2000 µg/mL, and 10-1000 µg/mL, respectively, for tyrosol; and 0.48-7.8 µg/mL, 0.25-16 µg/mL, and 0.125-4 µg/mL, respectively for β-lapachone. These compounds showed a decrease in MIC when the samples were subjected to osmotic stress, suggesting that the compounds acted on the fungal membrane. All the compounds were able to reduce the ergosterol content of the fungal strains. Finally, tyrosol was able to cause a leakage of intracellular molecules. |
publisher |
Sociedade Brasileira de Microbiologia |
publishDate |
2016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400917 |
work_keys_str_mv |
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