Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil
Paracoccidioides brasiliensis is the etiological agent of the major systemic mycosis in Brazil, called paracoccidioidomycosis. Although the Rio Grande do Sul is considered an endemic area of the disease, there are few studies on the ecology of P. brasiliensis in the state. Therefore, this study aimed to evaluate the infection of P. brasiliensis in horses from the mesoregion of Southwest Riograndense, using these animals as sentinels. Serological techniques, such as double immunodiffusion in agar gel (AGID) and indirect ELISA, were performed to detect the anti-gp43 P. brasiliensis antibody in horses from five different farms in the region of Bagé, RS, Brazil. Serology was performed in 200 Pure Blood English horses up to two years of age that were born and raised exclusively at the farms. Of these horses, 12% had anti-gp43 antibodies according to the ELISA results, with rates ranging from 0 to 30% according to the farm of origin (p < 0.001). Based on the immunodiffusion results, all equine serum samples were negative. These results indicate the presence of the fungus P. brasiliensis in the middle region of the southwestern state of Rio Grande do Sul, Brazil.
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Sociedade Brasileira de Microbiologia
2015
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oai:scielo:S1517-838220150002005132015-11-27Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, BrazilAlbano,Ana Paula NeuschrankKlafke,Gabriel BaracyBrandolt,Tchana MartinezDa Hora,Vanusa PousadaNogueira,Carlos Eduardo WayneXavier,Melissa OrzechowskiMeireles,Mário Carlos Araújo ELISA gp-43 protein G-peroxidase paracoccidioidomycosis Paracoccidioides brasiliensis is the etiological agent of the major systemic mycosis in Brazil, called paracoccidioidomycosis. Although the Rio Grande do Sul is considered an endemic area of the disease, there are few studies on the ecology of P. brasiliensis in the state. Therefore, this study aimed to evaluate the infection of P. brasiliensis in horses from the mesoregion of Southwest Riograndense, using these animals as sentinels. Serological techniques, such as double immunodiffusion in agar gel (AGID) and indirect ELISA, were performed to detect the anti-gp43 P. brasiliensis antibody in horses from five different farms in the region of Bagé, RS, Brazil. Serology was performed in 200 Pure Blood English horses up to two years of age that were born and raised exclusively at the farms. Of these horses, 12% had anti-gp43 antibodies according to the ELISA results, with rates ranging from 0 to 30% according to the farm of origin (p < 0.001). Based on the immunodiffusion results, all equine serum samples were negative. These results indicate the presence of the fungus P. brasiliensis in the middle region of the southwestern state of Rio Grande do Sul, Brazil.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology v.46 n.2 20152015-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200513en10.1590/S1517-838246246220140559 |
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Albano,Ana Paula Neuschrank Klafke,Gabriel Baracy Brandolt,Tchana Martinez Da Hora,Vanusa Pousada Nogueira,Carlos Eduardo Wayne Xavier,Melissa Orzechowski Meireles,Mário Carlos Araújo |
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Albano,Ana Paula Neuschrank Klafke,Gabriel Baracy Brandolt,Tchana Martinez Da Hora,Vanusa Pousada Nogueira,Carlos Eduardo Wayne Xavier,Melissa Orzechowski Meireles,Mário Carlos Araújo Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil |
author_facet |
Albano,Ana Paula Neuschrank Klafke,Gabriel Baracy Brandolt,Tchana Martinez Da Hora,Vanusa Pousada Nogueira,Carlos Eduardo Wayne Xavier,Melissa Orzechowski Meireles,Mário Carlos Araújo |
author_sort |
Albano,Ana Paula Neuschrank |
title |
Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil |
title_short |
Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil |
title_full |
Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil |
title_fullStr |
Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil |
title_full_unstemmed |
Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil |
title_sort |
seroepidemiology of paracoccidioides brasiliensis infection in horses from rio grande do sul, brazil |
description |
Paracoccidioides brasiliensis is the etiological agent of the major systemic mycosis in Brazil, called paracoccidioidomycosis. Although the Rio Grande do Sul is considered an endemic area of the disease, there are few studies on the ecology of P. brasiliensis in the state. Therefore, this study aimed to evaluate the infection of P. brasiliensis in horses from the mesoregion of Southwest Riograndense, using these animals as sentinels. Serological techniques, such as double immunodiffusion in agar gel (AGID) and indirect ELISA, were performed to detect the anti-gp43 P. brasiliensis antibody in horses from five different farms in the region of Bagé, RS, Brazil. Serology was performed in 200 Pure Blood English horses up to two years of age that were born and raised exclusively at the farms. Of these horses, 12% had anti-gp43 antibodies according to the ELISA results, with rates ranging from 0 to 30% according to the farm of origin (p < 0.001). Based on the immunodiffusion results, all equine serum samples were negative. These results indicate the presence of the fungus P. brasiliensis in the middle region of the southwestern state of Rio Grande do Sul, Brazil. |
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Sociedade Brasileira de Microbiologia |
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2015 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200513 |
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