Antifungal activity against Cryptococcus neoformans strains and genotoxicity assessment in human leukocyte cells of Euphorbia tirucalli L.
In the last times, focus on plant research has increased all over the world. Euphorbia tirucalli L., a plant known popularly as Aveloz, and originally used in Africa, has been drawing attention for its use in the United States and Latin America, both for use as an ornamental plant and as a medicinal plant. E. tirucalli L. is a member of the family Euphorbiaceae and contains many diterpenoids and triterpenoids, in particular phorbol esters, apparently the main constituent of this plant, which are assumed to be responsible for their activities in vivo and in vitro. The in vitro antifungal activities of Euphorbia tirucalli (L.) against opportunistic yeasts were studied using microbroth dilution assay. The results showed that aqueous extract and latex preparation were effective against ten clinical strains of Cryptococcus neoformans in vitro (Latex and extract MIC range of 3.2 - > 411 µg/mL). Aiming the safe use in humans, the genotoxic effects of E. tirucalli were evaluated in human leukocytes cells. Our data show that both aqueous extract and latex preparation have no genotoxic effect in human leukocytes cells in vitro. Although the results cannot be extrapolated by itself for use in vivo, they suggest a good perspective for a therapeutic application in future. In conclusion, our results show that the aqueous extract and latex preparation from E. tirucalli L. are antifungal agents effectives against several strains of C. neoformans and do not provoke DNA damage in human leukocyte cells, considering the concentrations tested.
Main Authors: | Oliveira,Luís Flávio Souza de, Fuentefria,Alexandre Meneghello, Klein,Fernanda da Silva, Machado,Michel Mansur |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Microbiologia
2014
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400027 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Evaluation of genotoxic and cytotoxic effects of hydroalcoholic extract of Euphorbia tirucalli (Euphorbiaceae) in cell cultures of human leukocytes
by: Machado,Michel M., et al.
Published: (2016) -
Synergistic effect of ibuprofen with itraconazole and fluconazole against Cryptococcus neoformans
by: Rocha,Letícia Fernandes da, et al.
Published: (2020) -
Antifungal activity of liriodenine on clinical strains of Cryptococcus neoformans and Cryptococcus gattii species complexes
by: Levorato-Vinche,Adriele Dandara, et al.
Published: (2022) -
Antifungal potential of punicalagin against Cryptococcus neoformans species complex
by: Silva,Thaísa Cristina, et al.
Published: (2018) -
Exploring antifungal potential: the flavonoid composition of Machaerium villosum extracts against Cryptococcus neoformans.
by: DOURADO, C. P., et al.
Published: (2024-04-24)