Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil
Milk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of Maringa, Parana State, Brazil were collected. Milk samples were decontaminated using 5% oxalic acid method and cultured on Lowenstein-Jensen and Stonebrink media at 35 °C and 30 °C, with and without 5-10% CO2. Mycobacteria isolates were identified by morphological features, PCR-Restriction Fragment Length Polymorphism Analysis (PCR-PRA) and Mycolic acids analysis. Thirteen (25%) raw and 2 (4%) pasteurized milk samples were positive for acid fast bacilli growth. Nine different species of NTM were isolated (M. nonchromogenicum, M. peregrinum, M. smegmatis, M. neoaurum, M. fortuitum, M. chelonae, M. flavescens, M. kansasii and M. scrofulaceum). M. bovis was not detected. Raw and pasteurized milk may be considered one source for NTM human infection. The paper reinforces the need for intensification of measures in order to avoid the milk contamination and consequently prevent diseases in the south of Brazil.
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Sociedade Brasileira de Microbiologia
2014
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oai:scielo:S1517-838220140002000462014-09-30Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, BrazilSgarioni,Sônia AparecidaHirata,Rosario Dominguez CrespoHiroyuki Hirata,MarioLeite,Clarice Queico FujimuraPrince,KarinaAndrade deLeite,Sergio Roberto de AndradeVedovello Filho,DirceuSiqueira,Vera Lucia DiasCaleffi-Ferracioli,Katiany RizzieriCardoso,Rosilene Fressatti non-tuberculous mycobacteria milk Mycobacterium PCR-PRA mycolic acids analysis Milk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of Maringa, Parana State, Brazil were collected. Milk samples were decontaminated using 5% oxalic acid method and cultured on Lowenstein-Jensen and Stonebrink media at 35 °C and 30 °C, with and without 5-10% CO2. Mycobacteria isolates were identified by morphological features, PCR-Restriction Fragment Length Polymorphism Analysis (PCR-PRA) and Mycolic acids analysis. Thirteen (25%) raw and 2 (4%) pasteurized milk samples were positive for acid fast bacilli growth. Nine different species of NTM were isolated (M. nonchromogenicum, M. peregrinum, M. smegmatis, M. neoaurum, M. fortuitum, M. chelonae, M. flavescens, M. kansasii and M. scrofulaceum). M. bovis was not detected. Raw and pasteurized milk may be considered one source for NTM human infection. The paper reinforces the need for intensification of measures in order to avoid the milk contamination and consequently prevent diseases in the south of Brazil.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology v.45 n.2 20142014-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200046en10.1590/S1517-83822014000200046 |
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Sgarioni,Sônia Aparecida Hirata,Rosario Dominguez Crespo Hiroyuki Hirata,Mario Leite,Clarice Queico Fujimura Prince,KarinaAndrade de Leite,Sergio Roberto de Andrade Vedovello Filho,Dirceu Siqueira,Vera Lucia Dias Caleffi-Ferracioli,Katiany Rizzieri Cardoso,Rosilene Fressatti |
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Sgarioni,Sônia Aparecida Hirata,Rosario Dominguez Crespo Hiroyuki Hirata,Mario Leite,Clarice Queico Fujimura Prince,KarinaAndrade de Leite,Sergio Roberto de Andrade Vedovello Filho,Dirceu Siqueira,Vera Lucia Dias Caleffi-Ferracioli,Katiany Rizzieri Cardoso,Rosilene Fressatti Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil |
author_facet |
Sgarioni,Sônia Aparecida Hirata,Rosario Dominguez Crespo Hiroyuki Hirata,Mario Leite,Clarice Queico Fujimura Prince,KarinaAndrade de Leite,Sergio Roberto de Andrade Vedovello Filho,Dirceu Siqueira,Vera Lucia Dias Caleffi-Ferracioli,Katiany Rizzieri Cardoso,Rosilene Fressatti |
author_sort |
Sgarioni,Sônia Aparecida |
title |
Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil |
title_short |
Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil |
title_full |
Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil |
title_fullStr |
Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil |
title_full_unstemmed |
Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil |
title_sort |
occurrence of mycobacterium bovis and non-tuberculous mycobacteria (ntm) in raw and pasteurized milk in the northwestern region of paraná, brazil |
description |
Milk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of Maringa, Parana State, Brazil were collected. Milk samples were decontaminated using 5% oxalic acid method and cultured on Lowenstein-Jensen and Stonebrink media at 35 °C and 30 °C, with and without 5-10% CO2. Mycobacteria isolates were identified by morphological features, PCR-Restriction Fragment Length Polymorphism Analysis (PCR-PRA) and Mycolic acids analysis. Thirteen (25%) raw and 2 (4%) pasteurized milk samples were positive for acid fast bacilli growth. Nine different species of NTM were isolated (M. nonchromogenicum, M. peregrinum, M. smegmatis, M. neoaurum, M. fortuitum, M. chelonae, M. flavescens, M. kansasii and M. scrofulaceum). M. bovis was not detected. Raw and pasteurized milk may be considered one source for NTM human infection. The paper reinforces the need for intensification of measures in order to avoid the milk contamination and consequently prevent diseases in the south of Brazil. |
publisher |
Sociedade Brasileira de Microbiologia |
publishDate |
2014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200046 |
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