Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.

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Bibliographic Details
Main Authors: Leal,C.A.G., Carvalho-Castro,G.A., Cottorello,A.C., Leite,R.C., Figueiredo,H.C.P.
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Microbiologia 2013
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300038
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