Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.

Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.

Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 1:3 moisture content, 20% inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50ºC and 6 respectively. The enzyme was found to be thermostable at 70ºC for about 2 h without any salt. It showed stability at pH range 5-7. The metal ions as Na+, Ca++, Mg++ and Co++ enhanced the enzyme activity.

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Main Authors: Saxena,Rajshree, Singh,Rajni
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Microbiologia 2011
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400014
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spelling oai:scielo:S1517-838220110004000142015-06-30Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.Saxena,RajshreeSingh,Rajni Solid State Fermentation Agro-industrial wastes Amylase Enzyme optimization Stability Bacillus sp Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 1:3 moisture content, 20% inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50ºC and 6 respectively. The enzyme was found to be thermostable at 70ºC for about 2 h without any salt. It showed stability at pH range 5-7. The metal ions as Na+, Ca++, Mg++ and Co++ enhanced the enzyme activity.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology v.42 n.4 20112011-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400014en10.1590/S1517-83822011000400014
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Saxena,Rajshree
Singh,Rajni
spellingShingle Saxena,Rajshree
Singh,Rajni
Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.
author_facet Saxena,Rajshree
Singh,Rajni
author_sort Saxena,Rajshree
title Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.
title_short Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.
title_full Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.
title_fullStr Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.
title_full_unstemmed Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.
title_sort amylase production by solid-state fermentation of agro-industrial wastes using bacillus sp.
description Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 1:3 moisture content, 20% inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50ºC and 6 respectively. The enzyme was found to be thermostable at 70ºC for about 2 h without any salt. It showed stability at pH range 5-7. The metal ions as Na+, Ca++, Mg++ and Co++ enhanced the enzyme activity.
publisher Sociedade Brasileira de Microbiologia
publishDate 2011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400014
work_keys_str_mv AT saxenarajshree amylaseproductionbysolidstatefermentationofagroindustrialwastesusingbacillussp
AT singhrajni amylaseproductionbysolidstatefermentationofagroindustrialwastesusingbacillussp
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