Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cells
Isolation of BCoV was performed in monolayers of HmLu-1 cells, using 20 fecal samples from clinical cases of diarrhea in calves. Samples were positive for BCoV by means of hemagglutination / hemagglutination inhibition (HA/HI). Up to the fifth serial passage, 13 of these isolates presented syncytial cytopathic effect, similar to Kakegawa standard strain. When isolates were submitted to seroneutralization with gammaglobulin anti-bovine coronavirus, 8 of them were considered to be positive, once cytopathic effect was neutralized. After titration and seroneutralization in microplates, only three of them were confirmed as positive. The lineage HmLu-1 showed higher permissivity to BCoV isolation, but the low intensity of viral replication demonstrated that new methodologies should be developed for this purpose and then submitted to confirmation of isolation by means of seroneutralization.
Main Authors: | Jerez,José Antonio, Gregori,Fábio, Brandão,Paulo Eduardo, Rodriguez,Cesar Alejandro Rosales, Ito,Fumio Honma, Buzinaro,Maria da Glória, Sakai,Takeo |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Microbiologia
2005
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300001 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Winter dysentery in cows associated with Bovine Coronavirus (BCoV)
by: Brandão,P.E., et al.
Published: (2007) -
Differentiation of Bovine coronavirus (BCoV) genotypes by a restriction enzyme assay
by: Souza,Sibele Pinheiro de, et al.
Published: (2010) -
Susceptibility of Hamster lung (HmLu) line to foot-and-mouth disease virus
by: Stouraitis, P., et al.
Published: (1975) -
Susceptibility of baby mice to FMD virus produced in BHK21 C13 and HmLu cells
by: Stouraitis, P., et al.
Published: (1975) -
Isolation in HRT-18 cells and molecular analysis of a BCoV strain from diarrheic feces of naturally infected calves
by: Stipp,Danilo Tancler, et al.
Published: (2009)